FR2324005A1 - Analytical method, esp. for thyroxine, using proteolytic enzyme - to release active component from binding protein - Google Patents

Analytical method, esp. for thyroxine, using proteolytic enzyme - to release active component from binding protein

Info

Publication number
FR2324005A1
FR2324005A1 FR7627563A FR7627563A FR2324005A1 FR 2324005 A1 FR2324005 A1 FR 2324005A1 FR 7627563 A FR7627563 A FR 7627563A FR 7627563 A FR7627563 A FR 7627563A FR 2324005 A1 FR2324005 A1 FR 2324005A1
Authority
FR
France
Prior art keywords
thyroxine
binding protein
tbg
mixt
esp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7627563A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of FR2324005A1 publication Critical patent/FR2324005A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Abstract

Process for analysing a serum sample for a non-protein cpd., in which the non-protein mols. are released from a binding protein comprises incubating the serum with an acid pepsin soln. and then adjusting the pH to >=6 to inactivate the pepsin. Pref. the determination of thyroxine (T4) content of a serum sample comprises incubating the sample with an enzyme soln. to release the T4 from T4-binding globulin (TBG). The mixt. is then treated with a TBG-T4-I125 reagent and contacted with a material contg. secondary binding sites. The mixt. is incubated and its initial radioactivity is measured. After this, the material with sec. binding sites is removed, washed, and its residual radioactivity measured. Pref. determination of the triiodothryonine (T3) content of a serum sample comprises incubating the sample with an enzyme soln. to release the T3 from TBG. The mixt. is then treated with a T3-antibody-T3-I125 reagent and treated as thyroxine (T4). A part from the determn. of T3 and T4, process (A) can also be used for the determn. of vitamin B12, steroids and other hormones and drugs.
FR7627563A 1975-09-15 1976-09-14 Analytical method, esp. for thyroxine, using proteolytic enzyme - to release active component from binding protein Withdrawn FR2324005A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US61315875A 1975-09-15 1975-09-15

Publications (1)

Publication Number Publication Date
FR2324005A1 true FR2324005A1 (en) 1977-04-08

Family

ID=24456111

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7627563A Withdrawn FR2324005A1 (en) 1975-09-15 1976-09-14 Analytical method, esp. for thyroxine, using proteolytic enzyme - to release active component from binding protein

Country Status (3)

Country Link
JP (1) JPS5236091A (en)
DE (1) DE2641313A1 (en)
FR (1) FR2324005A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0051985A2 (en) * 1980-11-07 1982-05-19 International Institute Of Cellular And Molecular Pathology (Icp) Immunoassay of proteins

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0051985A2 (en) * 1980-11-07 1982-05-19 International Institute Of Cellular And Molecular Pathology (Icp) Immunoassay of proteins
EP0051985A3 (en) * 1980-11-07 1982-08-11 Technicon Instruments Corporation Immunoassay of proteins

Also Published As

Publication number Publication date
JPS5236091A (en) 1977-03-19
DE2641313A1 (en) 1977-04-07

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