FI920760A0 - In vitro packningsystem foer kloning av dna-fragment med 95 kb. - Google Patents

In vitro packningsystem foer kloning av dna-fragment med 95 kb.

Info

Publication number
FI920760A0
FI920760A0 FI920760A FI920760A FI920760A0 FI 920760 A0 FI920760 A0 FI 920760A0 FI 920760 A FI920760 A FI 920760A FI 920760 A FI920760 A FI 920760A FI 920760 A0 FI920760 A0 FI 920760A0
Authority
FI
Finland
Prior art keywords
packningsystem
cloning
vitro
dna fragment
fragment
Prior art date
Application number
FI920760A
Other languages
English (en)
Inventor
Nat Leon Sternberg
Brian Lee Sauer
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of FI920760A0 publication Critical patent/FI920760A0/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI920760A 1989-08-22 1992-02-21 In vitro packningsystem foer kloning av dna-fragment med 95 kb. FI920760A0 (fi)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1989/003607 WO1991002801A1 (en) 1989-08-22 1989-08-22 An in vitro packaging system for cloning dna fragments of 95 kb

Publications (1)

Publication Number Publication Date
FI920760A0 true FI920760A0 (fi) 1992-02-21

Family

ID=22215180

Family Applications (1)

Application Number Title Priority Date Filing Date
FI920760A FI920760A0 (fi) 1989-08-22 1992-02-21 In vitro packningsystem foer kloning av dna-fragment med 95 kb.

Country Status (6)

Country Link
EP (1) EP0489002B1 (fi)
JP (1) JPH05503626A (fi)
DE (1) DE68920335T2 (fi)
FI (1) FI920760A0 (fi)
NO (1) NO920704L (fi)
WO (1) WO1991002801A1 (fi)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5378618A (en) * 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
DE68928062T2 (de) * 1988-04-15 1997-10-09 Du Pont Merck Pharma P1-bakteriophagen-klonierungssystem für bis zu 95 kb grossen dna-fragmenten
GB9206318D0 (en) * 1992-03-24 1992-05-06 Cambridge Antibody Tech Binding substances
EP0656941B1 (en) * 1992-03-24 2005-06-01 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US5888732A (en) 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
IL135776A0 (en) 1997-10-24 2001-05-20 Life Technologies Inc Recombinational cloning using nucleic acids having recombination sites
EP0916733A1 (de) * 1997-11-17 1999-05-19 Roche Diagnostics GmbH Verfahren und Kit zur Klonierung von Fragmenten
EP0919623A3 (en) * 1997-11-17 1999-07-14 Roche Diagnostics GmbH Procedure and kit for cloning of DNA fragments
JP4153607B2 (ja) 1998-11-18 2008-09-24 大日本住友製薬株式会社 新規な組換えアデノウイルス
AU774643B2 (en) 1999-03-02 2004-07-01 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
JP2003510019A (ja) * 1999-07-14 2003-03-18 クロンテック・ラボラトリーズ・インコーポレーテッド リコンビナーゼを利用した発現ベクターを作製する方法、およびこの方法を実施するために使用する組成物
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20050090010A1 (en) * 2001-03-02 2005-04-28 Yoshihide Hayashizaki Cloning vectors and method for molecular cloning
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
CN118028194A (zh) * 2023-12-26 2024-05-14 浙江大学 基因组精简的恶臭假单胞菌菌株

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68928062T2 (de) * 1988-04-15 1997-10-09 Du Pont Merck Pharma P1-bakteriophagen-klonierungssystem für bis zu 95 kb grossen dna-fragmenten

Also Published As

Publication number Publication date
DE68920335D1 (de) 1995-02-09
EP0489002A1 (en) 1992-06-10
JPH05503626A (ja) 1993-06-17
EP0489002B1 (en) 1994-12-28
NO920704L (no) 1992-04-15
WO1991002801A1 (en) 1991-03-07
DE68920335T2 (de) 1995-06-29
NO920704D0 (no) 1992-02-21

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Legal Events

Date Code Title Description
FD Application lapsed