FI920760A0 - In vitro packningsystem foer kloning av dna-fragment med 95 kb. - Google Patents
In vitro packningsystem foer kloning av dna-fragment med 95 kb.Info
- Publication number
- FI920760A0 FI920760A0 FI920760A FI920760A FI920760A0 FI 920760 A0 FI920760 A0 FI 920760A0 FI 920760 A FI920760 A FI 920760A FI 920760 A FI920760 A FI 920760A FI 920760 A0 FI920760 A0 FI 920760A0
- Authority
- FI
- Finland
- Prior art keywords
- packningsystem
- cloning
- vitro
- dna fragment
- fragment
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1989/003607 WO1991002801A1 (en) | 1989-08-22 | 1989-08-22 | An in vitro packaging system for cloning dna fragments of 95 kb |
Publications (1)
Publication Number | Publication Date |
---|---|
FI920760A0 true FI920760A0 (fi) | 1992-02-21 |
Family
ID=22215180
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI920760A FI920760A0 (fi) | 1989-08-22 | 1992-02-21 | In vitro packningsystem foer kloning av dna-fragment med 95 kb. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0489002B1 (fi) |
JP (1) | JPH05503626A (fi) |
DE (1) | DE68920335T2 (fi) |
FI (1) | FI920760A0 (fi) |
NO (1) | NO920704L (fi) |
WO (1) | WO1991002801A1 (fi) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5378618A (en) * | 1988-04-15 | 1995-01-03 | E. I. Du Pont De Nemours And Company | Vitro headful packaging system for cloning DNA fragments as large as 95kb |
DE68928062T2 (de) * | 1988-04-15 | 1997-10-09 | Du Pont Merck Pharma | P1-bakteriophagen-klonierungssystem für bis zu 95 kb grossen dna-fragmenten |
GB9206318D0 (en) * | 1992-03-24 | 1992-05-06 | Cambridge Antibody Tech | Binding substances |
EP0656941B1 (en) * | 1992-03-24 | 2005-06-01 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US6964861B1 (en) | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
US5888732A (en) | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
IL135776A0 (en) | 1997-10-24 | 2001-05-20 | Life Technologies Inc | Recombinational cloning using nucleic acids having recombination sites |
EP0916733A1 (de) * | 1997-11-17 | 1999-05-19 | Roche Diagnostics GmbH | Verfahren und Kit zur Klonierung von Fragmenten |
EP0919623A3 (en) * | 1997-11-17 | 1999-07-14 | Roche Diagnostics GmbH | Procedure and kit for cloning of DNA fragments |
JP4153607B2 (ja) | 1998-11-18 | 2008-09-24 | 大日本住友製薬株式会社 | 新規な組換えアデノウイルス |
AU774643B2 (en) | 1999-03-02 | 2004-07-01 | Invitrogen Corporation | Compositions and methods for use in recombinational cloning of nucleic acids |
JP2003510019A (ja) * | 1999-07-14 | 2003-03-18 | クロンテック・ラボラトリーズ・インコーポレーテッド | リコンビナーゼを利用した発現ベクターを作製する方法、およびこの方法を実施するために使用する組成物 |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US20050090010A1 (en) * | 2001-03-02 | 2005-04-28 | Yoshihide Hayashizaki | Cloning vectors and method for molecular cloning |
DE602004027538D1 (de) | 2003-12-01 | 2010-07-15 | Life Technologies Corp | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
CN118028194A (zh) * | 2023-12-26 | 2024-05-14 | 浙江大学 | 基因组精简的恶臭假单胞菌菌株 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE68928062T2 (de) * | 1988-04-15 | 1997-10-09 | Du Pont Merck Pharma | P1-bakteriophagen-klonierungssystem für bis zu 95 kb grossen dna-fragmenten |
-
1989
- 1989-08-22 WO PCT/US1989/003607 patent/WO1991002801A1/en active IP Right Grant
- 1989-08-22 EP EP89910354A patent/EP0489002B1/en not_active Expired - Lifetime
- 1989-08-22 DE DE68920335T patent/DE68920335T2/de not_active Expired - Lifetime
- 1989-08-22 JP JP1509694A patent/JPH05503626A/ja active Pending
-
1992
- 1992-02-21 NO NO92920704A patent/NO920704L/no unknown
- 1992-02-21 FI FI920760A patent/FI920760A0/fi not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
DE68920335D1 (de) | 1995-02-09 |
EP0489002A1 (en) | 1992-06-10 |
JPH05503626A (ja) | 1993-06-17 |
EP0489002B1 (en) | 1994-12-28 |
NO920704L (no) | 1992-04-15 |
WO1991002801A1 (en) | 1991-03-07 |
DE68920335T2 (de) | 1995-06-29 |
NO920704D0 (no) | 1992-02-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD | Application lapsed |