DE68920335D1 - In vitro-verpackungsvorrichtung um dna-fragmente von 95-kb zu klonen. - Google Patents

In vitro-verpackungsvorrichtung um dna-fragmente von 95-kb zu klonen.

Info

Publication number
DE68920335D1
DE68920335D1 DE68920335T DE68920335T DE68920335D1 DE 68920335 D1 DE68920335 D1 DE 68920335D1 DE 68920335 T DE68920335 T DE 68920335T DE 68920335 T DE68920335 T DE 68920335T DE 68920335 D1 DE68920335 D1 DE 68920335D1
Authority
DE
Germany
Prior art keywords
clone
dna fragments
packaging device
vitro packaging
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68920335T
Other languages
English (en)
Other versions
DE68920335T2 (de
Inventor
Nat Sternberg
Brian Sauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Health Sciences Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of DE68920335D1 publication Critical patent/DE68920335D1/de
Publication of DE68920335T2 publication Critical patent/DE68920335T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE68920335T 1989-08-22 1989-08-22 In vitro-verpackungsvorrichtung um dna-fragmente von 95-kb zu klonen. Expired - Lifetime DE68920335T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1989/003607 WO1991002801A1 (en) 1989-08-22 1989-08-22 An in vitro packaging system for cloning dna fragments of 95 kb

Publications (2)

Publication Number Publication Date
DE68920335D1 true DE68920335D1 (de) 1995-02-09
DE68920335T2 DE68920335T2 (de) 1995-06-29

Family

ID=22215180

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68920335T Expired - Lifetime DE68920335T2 (de) 1989-08-22 1989-08-22 In vitro-verpackungsvorrichtung um dna-fragmente von 95-kb zu klonen.

Country Status (6)

Country Link
EP (1) EP0489002B1 (de)
JP (1) JPH05503626A (de)
DE (1) DE68920335T2 (de)
FI (1) FI920760A0 (de)
NO (1) NO920704L (de)
WO (1) WO1991002801A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3019344B2 (ja) * 1988-04-15 2000-03-13 エヌ・イー・エヌ・ライフ・サイエンス・プロダクツ・インコーポレイテツド ９５ｋｂ程度の大きさのｄｎａ断片用ｐ１バクテリオフアージクローニング系
US5378618A (en) * 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
GB9206318D0 (en) * 1992-03-24 1992-05-06 Cambridge Antibody Tech Binding substances
CA2131151A1 (en) * 1992-03-24 1994-09-30 Kevin S. Johnson Methods for producing members of specific binding pairs
ATE222289T1 (de) 1995-06-07 2002-08-15 Invitrogen Corp Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
IL135776A0 (en) 1997-10-24 2001-05-20 Life Technologies Inc Recombinational cloning using nucleic acids having recombination sites
EP0919623A3 (de) * 1997-11-17 1999-07-14 Roche Diagnostics GmbH Verfahren und Kit zur Klonierung von Fragmenten
EP0916733A1 (de) * 1997-11-17 1999-05-19 Roche Diagnostics GmbH Verfahren und Kit zur Klonierung von Fragmenten
JP4153607B2 (ja) * 1998-11-18 2008-09-24 大日本住友製薬株式会社 新規な組換えアデノウイルス
NZ514569A (en) 1999-03-02 2004-02-27 Invitrogen Corp Compositions and methods for use in recombinational cloning of nucleic acids
US6410317B1 (en) 1999-07-14 2002-06-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1366177A1 (de) * 2001-03-02 2003-12-03 Riken Klonierungsvektoren und verfahren zu molekularklonen
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
CN118028194A (zh) * 2023-12-26 2024-05-14 浙江大学 基因组精简的恶臭假单胞菌菌株

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3019344B2 (ja) * 1988-04-15 2000-03-13 エヌ・イー・エヌ・ライフ・サイエンス・プロダクツ・インコーポレイテツド ９５ｋｂ程度の大きさのｄｎａ断片用ｐ１バクテリオフアージクローニング系

Also Published As

Publication number Publication date
JPH05503626A (ja) 1993-06-17
DE68920335T2 (de) 1995-06-29
WO1991002801A1 (en) 1991-03-07
EP0489002B1 (de) 1994-12-28
FI920760A0 (fi) 1992-02-21
NO920704L (no) 1992-04-15
EP0489002A1 (de) 1992-06-10
NO920704D0 (no) 1992-02-21

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: NEN LIFE SCIENCE PRODUCTS, INC., BOSTON, MASS., US