FI71165C - Foerfarande foer framstaellning av toxoplasma-antigen undersoekning av toxoplasma-immunitet - Google Patents
Foerfarande foer framstaellning av toxoplasma-antigen undersoekning av toxoplasma-immunitet Download PDFInfo
- Publication number
- FI71165C FI71165C FI824280A FI824280A FI71165C FI 71165 C FI71165 C FI 71165C FI 824280 A FI824280 A FI 824280A FI 824280 A FI824280 A FI 824280A FI 71165 C FI71165 C FI 71165C
- Authority
- FI
- Finland
- Prior art keywords
- antigen
- cells
- toxoplasma
- culture
- toxoplasms
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (10)
1 Pntentkrav
1. Förfarande for framstalining av en toxoplasmisk antigen för detekte-ring av toxoplasmisk immunitet, varvid man inokulerar Toxoplasma gondii i 5 en cellkultur i ett lämpligt medium, och att man tager tillvara kultur-mediet som är befriat frän celler och toxoplasmer ocli som innehäller toxoplasmiska exo-antigener, kännetecknat därav, att man utför odlingen i närvaro av toxoplasmer i ett kulturmedium som är befriat frän djurserum, säsom särskilt kalvserum, att man eliminerar lämningarna 10 av toxoplasmerna och väggarna som härrör sig fran cellerna eller toxoplas-merna genom steriliserande filtrering, att viruspartiklarna som eventuellt är närvarande i den lösliga antigenen efter filtreringen, deaktiveras, fördelaktigt med formalin, och att den lösliga antigenen koncentreras efter deaktiveringen. 15
2. Förfarande enligt patentkravet 1, kännetecknat därav, att man utför en minst tvahundrafaldig koncentration.
3. Förfarande enligt nagotdera av patentkraven 1 eller 2, kanne-20 tecknat därav, att man använder en cellinje som härrör sig fran diploidceller frän människan, och särskilt cellinjen MRC 5.
4. Förfarande enligt nagotdera av patentkraven 1 eller 2, kännetecknat därav, att man använder en cellinje av heteroploida djur- 25 celler, och särskilt cellinjen VERO.
5. Förfarande enligt nagotdera av patentkraven 1 eller 2, kännetecknat därav, att man använder en kultur av primärceller. 30 6. Förfarande enligt nägot av patentkraven 1-5, kännetecknat därav, att man suspenderar antigenen i glycerol.
7. Förfarande enligt nägot av patentkraven 1-6, kännetecknat därav, att man utför cellkulturen efter inokulering under 6 dagar vid 37°C. 35
8. Förfarande enligt nägot av patentkraven 1-7, kännetecknat
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8123565 | 1981-12-17 | ||
FR8123565A FR2518407A1 (fr) | 1981-12-17 | 1981-12-17 | Nouvel antigene pour la recherche de l'immunite toxoplasmique et son procede de preparation |
Publications (4)
Publication Number | Publication Date |
---|---|
FI824280A0 FI824280A0 (fi) | 1982-12-14 |
FI824280L FI824280L (fi) | 1983-06-18 |
FI71165B FI71165B (fi) | 1986-08-14 |
FI71165C true FI71165C (fi) | 1986-11-24 |
Family
ID=9265121
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI824280A FI71165C (fi) | 1981-12-17 | 1982-12-14 | Foerfarande foer framstaellning av toxoplasma-antigen undersoekning av toxoplasma-immunitet |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0082745B1 (sv) |
JP (1) | JPS58126814A (sv) |
AR (1) | AR228806A1 (sv) |
AT (1) | ATE14274T1 (sv) |
AU (1) | AU554541B2 (sv) |
CA (1) | CA1206904A (sv) |
DE (1) | DE3264792D1 (sv) |
ES (1) | ES518127A0 (sv) |
FI (1) | FI71165C (sv) |
FR (1) | FR2518407A1 (sv) |
NO (1) | NO824242L (sv) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2564731B1 (fr) * | 1984-05-28 | 1986-10-10 | Ambroise Thomas Pierre | Nouvelles fractions exo-antigeniques toxoplasmiques, leur procede de preparation et leur application a la recherche de l'immunite toxoplasmique chez l'homme par tests cutanes |
FR2618680B1 (fr) * | 1987-07-31 | 1990-05-11 | Pasteur Institut | Antigenes d'excretion-secretion specifiques de toxoplasma gondii, leurs produits d'expression, leur procede d'obtention et leurs applications diagnostiques et prophylactiques |
FR2625099B1 (fr) * | 1987-12-24 | 1991-06-14 | Transgene Sa | Proteines et leur procede de preparation, sequences d'adn, anticorps et leur application, poxvirus, cellules transformees ou infectees et compositions pharmaceutiques utiles dans la prevention de la toxoplasmose |
SI1856159T1 (sl) | 2005-03-08 | 2010-08-31 | Sigma Tau Ind Farmaceuti | Kimerni rekombinantni antigeni toksoplazme gondii |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5444016A (en) * | 1977-09-13 | 1979-04-07 | Nippon Touketsu Kansou Kenkiyu | Latex coagulation and reaction reagent for detecting toxoplasma antibody and production thereof |
FR2482980A1 (fr) * | 1980-05-20 | 1981-11-27 | Biomerieux Sa | Procede d'obtention de preparations de toxoplasmes pour le diagnostic de la toxoplasmose, et preparations ainsi obtenues |
-
1981
- 1981-12-17 FR FR8123565A patent/FR2518407A1/fr active Granted
-
1982
- 1982-12-02 AT AT82402197T patent/ATE14274T1/de active
- 1982-12-02 DE DE8282402197T patent/DE3264792D1/de not_active Expired
- 1982-12-02 EP EP82402197A patent/EP0082745B1/fr not_active Expired
- 1982-12-10 AU AU91404/82A patent/AU554541B2/en not_active Ceased
- 1982-12-10 AR AR291563A patent/AR228806A1/es active
- 1982-12-13 ES ES518127A patent/ES518127A0/es active Granted
- 1982-12-14 JP JP57217930A patent/JPS58126814A/ja active Pending
- 1982-12-14 FI FI824280A patent/FI71165C/fi not_active IP Right Cessation
- 1982-12-16 NO NO824242A patent/NO824242L/no unknown
- 1982-12-16 CA CA000417915A patent/CA1206904A/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
EP0082745A1 (fr) | 1983-06-29 |
ES8308485A1 (es) | 1983-09-01 |
JPS58126814A (ja) | 1983-07-28 |
ES518127A0 (es) | 1983-09-01 |
FI71165B (fi) | 1986-08-14 |
FR2518407A1 (fr) | 1983-06-24 |
FI824280A0 (fi) | 1982-12-14 |
AR228806A1 (es) | 1983-04-15 |
FI824280L (fi) | 1983-06-18 |
AU9140482A (en) | 1983-06-23 |
AU554541B2 (en) | 1986-08-28 |
ATE14274T1 (de) | 1985-08-15 |
DE3264792D1 (en) | 1985-08-22 |
CA1206904A (en) | 1986-07-02 |
EP0082745B1 (fr) | 1985-07-17 |
NO824242L (no) | 1983-06-20 |
FR2518407B1 (sv) | 1984-05-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM | Patent lapsed |
Owner name: INSTITUT MERIEUX |