FI60235C - Foerfarande foer framstaellning av tumoercelltillvaext inhiberande ks-2-a substans - Google Patents
Foerfarande foer framstaellning av tumoercelltillvaext inhiberande ks-2-a substans Download PDFInfo
- Publication number
- FI60235C FI60235C FI780947A FI780947A FI60235C FI 60235 C FI60235 C FI 60235C FI 780947 A FI780947 A FI 780947A FI 780947 A FI780947 A FI 780947A FI 60235 C FI60235 C FI 60235C
- Authority
- FI
- Finland
- Prior art keywords
- substance
- mice
- acid
- buffer
- minutes
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/811—Interferon
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Claims (13)
1. Elementalanalys C: 39,5 *, H: 6,5 N: 1,1 £ aska: sp&r (0,4 #);
2. Molekylvikt 9000 - 3000 (genom ultrafiltrering) 7000 - 9000 (genom jämnviktstäthetsgradient centrifugering) 8000 - 3000 (med fluorescein polarieationsmetod) ;
3. Utseende amorft vitt pulver;
4. Sönderdelningspunkt 185°C (baserat p& mätning av bryningstemperaturen med kapil-larmetod under användning av kiselolja);
5. UV-spektrum Ingen egentlig maximalabsorption observerad (fig. 1)}
6. IR-spektrum (fig. 2) ;
7. Pg för vattenlösning av substansen är 7,25;
8. Löslighet Löslig i vatten, olöslig i etanol, aceton, n-hexan, n-butanol, fenol och andra organiska lösningsmedel;
9. Optiak vridning M l5 = +67,5 ± 2,0°(i H20), (C = 0,452 *);
10. Homogenitet a. Resultatet av centrifugering medenlinjär gradient av 5-20 % CsCl i o,1 M Tris-HCl buffert (pg 7,2) vid omloppstalet 38,000 varv/min och 4°C under 15 min visar att substansen är homogen (fig. 3); jämförande sedimenteringsförsök med virus-nuklein-syra visar att substansen icke innehäller viruspartiklar, RNA eller DNA; b. Substansen är elektroforetiskt homogen enligt fig. 4, som utvisar resultatet av elektrofores p& cellulosaacetat under användning av 0,1 M ättiksyra/pyridin buffert (p^ 3,5), varvid kondroitinaulfat användes som kontroll; 30 minuter efter 23 60235 elektroforesen vid 0,6 mA/cm och 160 V rör sig kondroitin-sulfatet tili katoden med en rörlighet av 4 cm per 30 min , raedan KS-2-A rör sig i nägon m&n tili anoden pä 90 min (en rörlighet av 1 cm per 90 min); substansen bar ocksä visat sig vara homogen genom elektrofores utförd under samma betingelser som ovan förutom att 1 M pyridin/ättiksyra buffert (pjj 7*0) användes;
11. Färgreaktion Fenol-H2S0^ reaktion positiv Antron reaktion ” Molisch’ reaktion " Elson-Morgens reaktion negativ Karbazol-HgO^ reaktion positiv Reaktion med Folin-Giocalteam " Biuret reaktion " Reaktion med FITC (fluorescein isotiocyanat) " Toluidin bldtt 0 färgning negativ Ninhydrin reaktion positiv
12. Sockerkomposition' Fig. 5 visar resultatet av att l&ta substansen undergä syra-hydrolys och päföljande reduktion med natriumborhydrid, sedan acetylering, varefter sockerkompositionen bestämdes genom gas-kromatografi; substansen bestär huvudsakligen av mannos och inneh&ller sm& mängder glukos och galaktos samt även mycket smä. mängder arabinos och xylos, varvid viktförh&llandena mellan dessa substanser är 74 s 12 s 12 : 1 : 1 ;
13. Aminosyrakomposition aminosyraanalys, som utförts i automatisk aminosyraanalysa-tor, genom att 10 mg av substansen inneslöts i vakuum till-sammans med 3 ml 6 N HC1 och underkastades syraanalys vid 110°C under 22 timmars tid, varefter HOI avlägsnades med roterande indunstare, visar att de i substansen befintliga aminosyrorna utgörs huvudsakligen av treonin, serin, glu-taminsyra och alanin samt dessutom sm& mängder av aspara-ginsyra, prolin, glycin, valin och lysin; vidare ndgongAng spärmängder av metionin, isoleucin, leuoin, tyrosin, fenyl-alanin, histidin, arginin och cystin;
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3594477 | 1977-03-30 | ||
| JP3594477A JPS53121919A (en) | 1977-03-30 | 1977-03-30 | Interferon inducing agent containing polysaccharide mainly |
| JP10632177 | 1977-09-06 | ||
| JP10632177A JPS5441801A (en) | 1977-09-06 | 1977-09-06 | Kss22a |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI780947A FI780947A (fi) | 1978-10-01 |
| FI60235B FI60235B (fi) | 1981-08-31 |
| FI60235C true FI60235C (fi) | 1981-12-10 |
Family
ID=26374963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI780947A FI60235C (fi) | 1977-03-30 | 1978-03-29 | Foerfarande foer framstaellning av tumoercelltillvaext inhiberande ks-2-a substans |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4163780A (sv) |
| CH (1) | CH641204A5 (sv) |
| DE (1) | DE2813353A1 (sv) |
| FI (1) | FI60235C (sv) |
| FR (1) | FR2385734A1 (sv) |
| GB (1) | GB1572074A (sv) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3071750D1 (en) * | 1979-07-04 | 1986-10-16 | Kureha Chemical Ind Co Ltd | Nasal preparations, process for making same and their use |
| JPS5712999A (en) * | 1980-06-25 | 1982-01-22 | Chiyokichi Iizuka | Antibiral agent and its preparation |
| US4512972A (en) * | 1980-06-30 | 1985-04-23 | Kureha Chemical Industry Co., Ltd. | Nasal preparation and processes for their production |
| WO1982003771A1 (fr) * | 1981-04-30 | 1982-11-11 | Mitsuhashi Susumu | Polysaccharide ps-a isole a partir de la plante appartenant au genre epidemium violaceum morr. et decne., procede de preparation, et agent de prevention de l'infection et stimulateur de l'immunite contenant le polysaccharide a titre d'ingredient efficace |
| EP0384798B1 (en) * | 1989-02-06 | 1993-08-11 | Gen-Ichiro Soma | Limulus test-positive plant glycolipid, and method of stimulating the immuno system of an animal |
| US7632651B2 (en) | 1997-09-15 | 2009-12-15 | Mds Analytical Technologies (Us) Inc. | Molecular modification assays |
| US7070921B2 (en) | 2000-04-28 | 2006-07-04 | Molecular Devices Corporation | Molecular modification assays |
| US7745142B2 (en) | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
| US6975892B2 (en) * | 2003-10-21 | 2005-12-13 | Oculir, Inc. | Methods for non-invasive analyte measurement from the conjunctiva |
| US6958039B2 (en) * | 2003-05-02 | 2005-10-25 | Oculir, Inc. | Method and instruments for non-invasive analyte measurement |
| US6968222B2 (en) | 2003-05-02 | 2005-11-22 | Oculir, Inc. | Methods and device for non-invasive analyte measurement |
| US20060224057A1 (en) * | 2003-10-21 | 2006-10-05 | Oculir, Inc. | Methods for non-invasive analyte measurement |
| US20080009688A1 (en) * | 2004-04-14 | 2008-01-10 | Oculir, Inc. | Methods for non-invasive analyte measurement |
| US20060258919A1 (en) * | 2004-04-14 | 2006-11-16 | Oculir, Inc. | Non-Invasive Analyte Measurement Device for Measuring Tears and Other Ocular Elements Using Electromagnetic Radiation and Method of Using the Same |
| CA2574060C (en) * | 2004-07-16 | 2019-06-25 | Medimush A/S | Immune modulating compounds from fungi |
| US7514085B2 (en) * | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| EP1885866A1 (en) * | 2005-05-13 | 2008-02-13 | Medimush A/S | Feed or food products comprising fungal material |
| WO2006133707A2 (en) * | 2005-06-15 | 2006-12-21 | Medimush A/S | Anti-cancer combination treatment and kit-of-part |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE757248A (fr) * | 1969-10-15 | 1971-04-08 | Kureha Chemical Ind Co Ltd | Substance a propriete anticancereuse et procedes pour sa preparation |
-
1978
- 1978-03-17 GB GB10622/78A patent/GB1572074A/en not_active Expired
- 1978-03-28 DE DE19782813353 patent/DE2813353A1/de not_active Withdrawn
- 1978-03-29 FR FR7809021A patent/FR2385734A1/fr active Granted
- 1978-03-29 FI FI780947A patent/FI60235C/fi not_active IP Right Cessation
- 1978-03-30 US US05/891,767 patent/US4163780A/en not_active Expired - Lifetime
- 1978-03-30 CH CH339178A patent/CH641204A5/fr not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| GB1572074A (en) | 1980-07-23 |
| FR2385734A1 (fr) | 1978-10-27 |
| FI60235B (fi) | 1981-08-31 |
| DE2813353A1 (de) | 1978-10-19 |
| FR2385734B1 (sv) | 1981-10-30 |
| CH641204A5 (fr) | 1984-02-15 |
| US4163780A (en) | 1979-08-07 |
| FI780947A (fi) | 1978-10-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MA | Patent expired | ||
| MA | Patent expired |
Owner name: KIRIN-SEAGRAM LIMITED |