FI3898985T3 - Proteiinien tandemekspressio - Google Patents

Proteiinien tandemekspressio Download PDF

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Publication number
FI3898985T3
FI3898985T3 FIEP19817783.4T FI19817783T FI3898985T3 FI 3898985 T3 FI3898985 T3 FI 3898985T3 FI 19817783 T FI19817783 T FI 19817783T FI 3898985 T3 FI3898985 T3 FI 3898985T3
Authority
FI
Finland
Prior art keywords
fusarium
polypeptides
interest
host cell
nucleic acid
Prior art date
Application number
FIEP19817783.4T
Other languages
English (en)
Finnish (fi)
Inventor
Richard Baerends
Carsten Olsen
Josè Arnau
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of FI3898985T3 publication Critical patent/FI3898985T3/fi

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
FIEP19817783.4T 2018-12-21 2019-12-17 Proteiinien tandemekspressio FI3898985T3 (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18215558 2018-12-21
PCT/EP2019/085553 WO2020127198A1 (en) 2018-12-21 2019-12-17 Tandem protein expression

Publications (1)

Publication Number Publication Date
FI3898985T3 true FI3898985T3 (fi) 2023-05-05

Family

ID=64959159

Family Applications (1)

Application Number Title Priority Date Filing Date
FIEP19817783.4T FI3898985T3 (fi) 2018-12-21 2019-12-17 Proteiinien tandemekspressio

Country Status (6)

Country Link
US (1) US20220073898A1 (zh)
EP (1) EP3898985B1 (zh)
CN (1) CN113316641A (zh)
DK (1) DK3898985T3 (zh)
FI (1) FI3898985T3 (zh)
WO (1) WO2020127198A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202221129A (zh) * 2020-08-10 2022-06-01 比利時商因比奧斯公司 雙醣及/或寡醣的細胞生產

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
MX9606013A (es) 1994-06-03 1997-12-31 Novo Nordisk Biotech Inc Lacasas purificadas de myceliophthora y acidos nucleicos que codifican para las mismas.
EP0777737B1 (en) 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
EP0785994A1 (en) * 1994-10-26 1997-07-30 Novo Nordisk A/S An enzyme with lipolytic activity
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
DE69939947D1 (de) 1998-12-23 2009-01-02 Novozymes As Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
EP2278016B1 (en) 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
JP5114194B2 (ja) * 2004-03-25 2013-01-09 ジェネンコー・インターナショナル・インク セルラーゼ融合タンパク質及びこれをコードする異種セルラーゼ融合構築体
US7527947B2 (en) * 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
US8415119B2 (en) * 2008-02-25 2013-04-09 Novozymes A/S Method for increasing expression yield of a protein of interest
WO2010039889A2 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
ES2631605T3 (es) * 2011-11-25 2017-09-01 Novozymes A/S Polipéptidos con actividad de lisozima y polinucleótidos que codifican los mismos
WO2013178674A1 (en) 2012-05-31 2013-12-05 Novozymes A/S Improved selection in fungi
EP3080254B1 (en) * 2013-12-11 2024-05-15 Novozymes A/S Cutinase variants and polynucleotides encoding same
CN104962573A (zh) * 2015-07-08 2015-10-07 北京嘉万生物技术有限公司 Pgⅱ与mbp融合蛋白的可溶性分泌表达及其应用
RU2763469C2 (ru) 2017-02-20 2021-12-29 Новозимс А/С Липолитический фермент для применения в хлебопечении

Also Published As

Publication number Publication date
EP3898985A1 (en) 2021-10-27
EP3898985B1 (en) 2023-02-08
DK3898985T3 (da) 2023-04-17
WO2020127198A1 (en) 2020-06-25
US20220073898A1 (en) 2022-03-10
CN113316641A (zh) 2021-08-27

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