FI120977B - Bakteriefermenteringsodling med hög celltäthet - Google Patents
Bakteriefermenteringsodling med hög celltäthet Download PDFInfo
- Publication number
- FI120977B FI120977B FI20095510A FI20095510A FI120977B FI 120977 B FI120977 B FI 120977B FI 20095510 A FI20095510 A FI 20095510A FI 20095510 A FI20095510 A FI 20095510A FI 120977 B FI120977 B FI 120977B
- Authority
- FI
- Finland
- Prior art keywords
- culture
- medium
- cell density
- phosphate
- high cell
- Prior art date
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- 230000004151 fermentation Effects 0.000 title claims abstract description 47
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factor [FGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Glass Compositions (AREA)
- Treatment Of Sludge (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (8)
1. Bakteriefermenteringsodling med stor celltät-het, kännetecknad av att den omfattar ett fosfatglas 5 som fosforkalla, varvid fosfatglaset minskar utfällningen av fosfat jämfört med celler som odlas i odlingssubstrat utan fosfatglas.
2. Odling enligt patentkraven 1, kännetecknad av att narnnda fosfatglas väljs frän en grupp, som bestär 10 av natriumfosfatglas med formeln P04 - (PO3) n - P04/ var vid kedjornas längder varierar mellan cirka n = 2 och cir-ka n = 100.
3. Odling enligt patentkrav 2, kännetecknad av att nämnda natriumfosfatglas har en kedjelängd pä cirka 15 n = 4 - cirka n = 20.
4. Odling enligt patentkrav 3, kännetecknad av att nämnda natriumfosfatglas har en kedjelängd pä n = 11.
5. Odling enligt patentkrav 1, kännetecknad 2. av att f ermenteringsodlingen med stor celltäthet väljs frän en grupp, som bestär av satsfermenteringsodling, kon-tinuerlig fermenteringsodling eller fed-batch-fermenteringsodling.
6. Odling enligt patentkrav 1, kännetecknad 25 av att odlingen med stor celltäthet ger ett utbyte, som är mera än 20 gram av cellens torrvikt/liter odlingssubstrat.
7. Odling enligt patentkrav 1, kännetecknad av att odlingssubstratet omfattar glukos i en koncentra-tion, som är 45 g/liter - 75 g/liter.
8. Odling enligt patentkrav 1, kännetecknad av att bakteriefermenteringsodlingen omfattar E. coli, som exprimerar ett rekombinantprotein.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23557394A | 1994-04-28 | 1994-04-28 | |
| US23557394 | 1994-04-28 | ||
| US9505294 | 1995-04-27 | ||
| PCT/US1995/005294 WO1995029986A1 (en) | 1994-04-28 | 1995-04-27 | Method for controlling metallophosphate precipitation in high cell density fermentations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FI20095510A FI20095510A (sv) | 2009-05-05 |
| FI120977B true FI120977B (sv) | 2010-05-31 |
Family
ID=22886063
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI964208A FI120405B (sv) | 1994-04-28 | 1996-10-18 | Förfarande för kontrollering av utfällningen av metallofosfat i fermenteringar med hög celltäthet |
| FI20095510A FI120977B (sv) | 1994-04-28 | 2009-05-05 | Bakteriefermenteringsodling med hög celltäthet |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI964208A FI120405B (sv) | 1994-04-28 | 1996-10-18 | Förfarande för kontrollering av utfällningen av metallofosfat i fermenteringar med hög celltäthet |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US7118884B1 (sv) |
| EP (1) | EP0755438B1 (sv) |
| JP (1) | JP3592330B2 (sv) |
| CN (1) | CN1105776C (sv) |
| AT (1) | ATE199166T1 (sv) |
| AU (1) | AU699554B2 (sv) |
| CA (1) | CA2188434C (sv) |
| DE (1) | DE69520098T2 (sv) |
| DK (1) | DK0755438T3 (sv) |
| ES (1) | ES2153896T3 (sv) |
| FI (2) | FI120405B (sv) |
| GR (1) | GR3035808T3 (sv) |
| IL (2) | IL113497A0 (sv) |
| MX (1) | MX9604878A (sv) |
| NO (1) | NO323554B1 (sv) |
| PT (1) | PT755438E (sv) |
| WO (1) | WO1995029986A1 (sv) |
| ZA (1) | ZA953452B (sv) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1654283B1 (en) | 2003-08-13 | 2011-07-13 | Novartis Vaccines and Diagnostics, Inc. | Improved method of purifying tfpi and tfpi analogs |
| GB0522303D0 (en) | 2005-11-01 | 2005-12-07 | Chiron Srl | Culture method |
| GB0818453D0 (en) | 2008-10-08 | 2008-11-12 | Novartis Ag | Fermentation processes for cultivating streptococci and purification processes for obtaining cps therefrom |
| US10237679B2 (en) * | 2008-06-27 | 2019-03-19 | Here Global B.V. | Method, apparatus, and computer program product for location sharing |
| US8213591B2 (en) * | 2008-11-13 | 2012-07-03 | At&T Intellectual Property I, L.P. | Methods, systems, and products for providing ring tones |
| EP2445924B2 (en) | 2009-06-25 | 2023-12-13 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| KR20230029769A (ko) | 2020-07-01 | 2023-03-03 | 론자 리미티드 | 미생물 배양 배지 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE290212C (sv) | ||||
| US3640827A (en) * | 1968-10-09 | 1972-02-08 | Fmc Corp | Phosphate glass bodies |
| US4727031A (en) * | 1984-11-08 | 1988-02-23 | International Technology Corporation | Nutrient for stimulating aerobic bacteria |
| US4906575A (en) * | 1987-03-06 | 1990-03-06 | Chevron Research Company | Phosphate compound that is used in a microbial profile modification process |
| US4947932A (en) * | 1987-03-06 | 1990-08-14 | Chevron Research Company | Phosphate compound that is used in a microbial profile modification process |
| DD290212A5 (de) | 1988-07-28 | 1991-05-23 | Zi Fuer Mikrobiologie Und Experimentelle Therapie,De | Verfahren zur herstellung eines glukose-minimalmediums fuer escherichia coli-massenkulturfermentation |
| US5128260A (en) * | 1989-01-12 | 1992-07-07 | Sanofi Bio Ingredients, Inc. | Process for preparing culture concentrates for direct vat set dairy products production |
| CA2078054A1 (en) | 1991-09-13 | 1993-03-14 | Sun Yukun | High-yield fermentation processes |
-
1995
- 1995-04-25 IL IL11439795A patent/IL113497A0/xx unknown
- 1995-04-25 IL IL11349795A patent/IL113497A/en not_active IP Right Cessation
- 1995-04-27 AT AT95917747T patent/ATE199166T1/de active
- 1995-04-27 JP JP52841295A patent/JP3592330B2/ja not_active Expired - Lifetime
- 1995-04-27 EP EP95917747A patent/EP0755438B1/en not_active Expired - Lifetime
- 1995-04-27 PT PT95917747T patent/PT755438E/pt unknown
- 1995-04-27 AU AU23691/95A patent/AU699554B2/en not_active Expired
- 1995-04-27 MX MX9604878A patent/MX9604878A/es unknown
- 1995-04-27 CA CA002188434A patent/CA2188434C/en not_active Expired - Lifetime
- 1995-04-27 DK DK95917747T patent/DK0755438T3/da active
- 1995-04-27 ES ES95917747T patent/ES2153896T3/es not_active Expired - Lifetime
- 1995-04-27 WO PCT/US1995/005294 patent/WO1995029986A1/en active Application Filing
- 1995-04-27 DE DE69520098T patent/DE69520098T2/de not_active Expired - Lifetime
- 1995-04-27 CN CN95193686A patent/CN1105776C/zh not_active Expired - Lifetime
- 1995-05-08 ZA ZA953452A patent/ZA953452B/xx unknown
-
1996
- 1996-08-21 US US08/701,032 patent/US7118884B1/en not_active Expired - Lifetime
- 1996-10-14 NO NO19964368A patent/NO323554B1/no not_active IP Right Cessation
- 1996-10-18 FI FI964208A patent/FI120405B/sv not_active IP Right Cessation
-
2001
- 2001-04-27 GR GR20010400656T patent/GR3035808T3/el unknown
-
2006
- 2006-10-04 US US11/543,323 patent/US7381545B2/en not_active Expired - Fee Related
-
2009
- 2009-05-05 FI FI20095510A patent/FI120977B/sv not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US7381545B2 (en) | 2008-06-03 |
| FI120405B (sv) | 2009-10-15 |
| CN1151182A (zh) | 1997-06-04 |
| ATE199166T1 (de) | 2001-02-15 |
| ES2153896T3 (es) | 2001-03-16 |
| NO964368L (no) | 1996-12-30 |
| WO1995029986A1 (en) | 1995-11-09 |
| EP0755438A1 (en) | 1997-01-29 |
| FI964208A0 (sv) | 1996-10-18 |
| CN1105776C (zh) | 2003-04-16 |
| IL113497A0 (en) | 1995-07-31 |
| CA2188434C (en) | 1999-08-31 |
| AU2369195A (en) | 1995-11-29 |
| JP3592330B2 (ja) | 2004-11-24 |
| NO323554B1 (no) | 2007-06-11 |
| EP0755438B1 (en) | 2001-02-14 |
| DE69520098D1 (de) | 2001-03-22 |
| DE69520098T2 (de) | 2001-07-19 |
| US20070026497A1 (en) | 2007-02-01 |
| JPH09512439A (ja) | 1997-12-16 |
| FI20095510A (sv) | 2009-05-05 |
| PT755438E (pt) | 2001-07-31 |
| ZA953452B (en) | 1996-01-17 |
| CA2188434A1 (en) | 1995-11-09 |
| IL113497A (en) | 2001-01-11 |
| NO964368D0 (no) | 1996-10-14 |
| GR3035808T3 (en) | 2001-07-31 |
| US7118884B1 (en) | 2006-10-10 |
| MX9604878A (es) | 1998-01-31 |
| AU699554B2 (en) | 1998-12-10 |
| FI964208A (sv) | 1996-10-18 |
| DK0755438T3 (da) | 2001-03-19 |
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