FI119476B - Expression av heterologa proteiner i försvagade bakterier genom att använda htrA-promotorer - Google Patents
Expression av heterologa proteiner i försvagade bakterier genom att använda htrA-promotorer Download PDFInfo
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- FI119476B FI119476B FI963016A FI963016A FI119476B FI 119476 B FI119476 B FI 119476B FI 963016 A FI963016 A FI 963016A FI 963016 A FI963016 A FI 963016A FI 119476 B FI119476 B FI 119476B
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/08—Clostridium, e.g. Clostridium tetani
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
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- Life Sciences & Earth Sciences (AREA)
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- Biotechnology (AREA)
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- Gastroenterology & Hepatology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (11)
1. Vaccinkomposition, som omfattar en forsvagad bakterie, som innehäller en DNA-konstrukt, som omfattar en 5 htrA-promotorsekvens funktionellt ansluten till en DNA-sekvens, som kodar för en eller flera heterologa protei-ner, och en farmaceutisk godtagbar bärare,
2. Vaccinkomposition enligt patentkrav 1, vari htrA-promotorsekvensen är funktionellt ansluten till en
10 DNA-sekvens, som kodar för tvä eller flera proteiners fu-sionsprotein.
3. Vaccinkomposition enligt patentkrav 2, väri de fusionsbildande proteinerna har sammanfogats med ett flex-ibelt ledomräde.
4. Vaccinkomposition enligt patentkrav 3, varvid ledomrädet är en kedja av fyra eller flera aminosyror med sekvensen - [X]P“Pro- [y]q-Pro-[Z]r- 20 , m*m vari Pro är prolin, X och Y är vardera glycin eller amino- • · · syra med en icke-stor sidokedja, Z är vilken aminosyra som ... heist, p är ett positivt heltal, q är ett positivt heltal, • · *««·* som är 1-10, och r är 0 eller ett positivt heltal, som är ·* · ϊ *·* 25 större än 0.
5, Vaccinkomposition enligt nägot av patentkraven ♦ *· ί(! ί 2,3 och 4, varyid promotorn är funktionellt ansluten tili DNA-sekvensen, som kodar för ett första och andra hetero-logt protein, varvid det första heterologa proteinet är en • · .***; 30 antigen sekvens, som omfattar ett C-fragment av tetanus- toxin eller en eller flera epitoper därav. ♦ · ·
6. Vaccinkomposition enligt nägot av de föregäende • · *·”* patentkraven, som är anpassad för administrering via mun- :***: nen eller näsan . • m :*·.· 35 7. Vaccinkomposition enligt nägot av de föregäende • · patentkraven, vari den försvagade bakterien försvagas pa 35 119476 grund av att den har en icke-reverterande mutation i en gen, som Sr pä den bakteriens aromatiska aminosyrors bio-syntetiska rutt.
8. Vaccinkomposition enligt patentkrav 7, varvid 5 nämnda försvagade bakterie dessutom har en icke- reverterande mutation i htrA-genen.
9. Vaccinkomposition enligt patentkrav 7 eller 8, vari den försvagade bakterien innehSller en icke-reverterande mutation i vardera tvä separata genen, som är pS 10 den bakteriens aromatiska aminosyrors biosyntetiska rutt.
10. Vaccinkomposition enligt patentkrav 7, 8 eller 9, varvid nämnda gen eller vardera av nämnda gener pä de aromatiska aminosyrornas biosyntetiska rutt väljs frSn arpA, aroC, aroD och aroE.
11. Vaccinkomposition enligt patentkrav 9, varvid nämnda tvä separata gener är aroA och aroD. • 1 · t a • 1 1 • · ··· • · • a • a • a a • a a • a • a • • a a « « · • •a «aa a a a « · · a • a • · · • · a • a • · · • · « a ♦ aa « ··« a a a • · · « « · a • « • · ··· a ·· · « · a • · • · a • · · • ·· t ·
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9401795 | 1994-01-31 | ||
GB9401795A GB9401795D0 (en) | 1994-01-31 | 1994-01-31 | Vaccines |
PCT/GB1995/000196 WO1995020665A1 (en) | 1994-01-31 | 1995-01-31 | Expression of heterologous proteins in attenuated bacteria using the htra-promoters |
GB9500196 | 1995-01-31 |
Publications (3)
Publication Number | Publication Date |
---|---|
FI963016A0 FI963016A0 (sv) | 1996-07-30 |
FI963016A FI963016A (sv) | 1996-09-25 |
FI119476B true FI119476B (sv) | 2008-11-28 |
Family
ID=10749598
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI963016A FI119476B (sv) | 1994-01-31 | 1996-07-30 | Expression av heterologa proteiner i försvagade bakterier genom att använda htrA-promotorer |
Country Status (19)
Country | Link |
---|---|
EP (1) | EP0742829B1 (sv) |
JP (1) | JP3731004B2 (sv) |
KR (1) | KR100392916B1 (sv) |
AT (1) | ATE254178T1 (sv) |
AU (1) | AU701384B2 (sv) |
BR (1) | BR9506666A (sv) |
CA (1) | CA2182315C (sv) |
CZ (1) | CZ285867B6 (sv) |
DE (1) | DE69532109T2 (sv) |
DK (1) | DK0742829T3 (sv) |
ES (1) | ES2210283T3 (sv) |
FI (1) | FI119476B (sv) |
GB (1) | GB9401795D0 (sv) |
HU (1) | HU222982B1 (sv) |
NZ (1) | NZ278930A (sv) |
PL (1) | PL180091B1 (sv) |
PT (1) | PT742829E (sv) |
SK (1) | SK99696A3 (sv) |
WO (1) | WO1995020665A1 (sv) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9720033D0 (en) * | 1997-09-19 | 1997-11-19 | Medeva Plc | Hepatitis B virus polypeptides |
US20020168771A1 (en) * | 2001-05-08 | 2002-11-14 | Gamerman Gary Eric | Vectors having replication, immunogenicity and/or pathogenicity under stress promoter regulation and use thereof |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU634153B2 (en) * | 1988-02-01 | 1993-02-18 | Wyeth Holdings Corporation | T-cell epitope as carriers molecule for conjugate vaccines |
CA2031468A1 (en) * | 1989-12-08 | 1991-06-09 | Mitchell S. Gross | Malaria vaccine |
GB9007194D0 (en) * | 1990-03-30 | 1990-05-30 | Wellcome Found | Live vaccines |
JP3415145B2 (ja) * | 1991-03-05 | 2003-06-09 | ザ ウエルカム ファウンデーション リミテッド | 弱毒化細菌における組換えタンパク質の発現 |
JP3633933B2 (ja) * | 1992-07-31 | 2005-03-30 | アカンビス、リサーチ、リミテッド | 弱毒化細菌における組換え融合タンパク質の発現 |
-
1994
- 1994-01-31 GB GB9401795A patent/GB9401795D0/en active Pending
-
1995
- 1995-01-31 PT PT95907080T patent/PT742829E/pt unknown
- 1995-01-31 EP EP95907080A patent/EP0742829B1/en not_active Expired - Lifetime
- 1995-01-31 WO PCT/GB1995/000196 patent/WO1995020665A1/en active IP Right Grant
- 1995-01-31 SK SK996-96A patent/SK99696A3/sk unknown
- 1995-01-31 AT AT95907080T patent/ATE254178T1/de active
- 1995-01-31 CA CA002182315A patent/CA2182315C/en not_active Expired - Lifetime
- 1995-01-31 NZ NZ278930A patent/NZ278930A/en not_active IP Right Cessation
- 1995-01-31 DK DK95907080T patent/DK0742829T3/da active
- 1995-01-31 ES ES95907080T patent/ES2210283T3/es not_active Expired - Lifetime
- 1995-01-31 JP JP51995795A patent/JP3731004B2/ja not_active Expired - Lifetime
- 1995-01-31 PL PL95315670A patent/PL180091B1/pl unknown
- 1995-01-31 AU AU15415/95A patent/AU701384B2/en not_active Ceased
- 1995-01-31 CZ CZ962182A patent/CZ285867B6/cs not_active IP Right Cessation
- 1995-01-31 KR KR1019960704245A patent/KR100392916B1/ko active IP Right Grant
- 1995-01-31 HU HU9602084A patent/HU222982B1/hu not_active IP Right Cessation
- 1995-01-31 BR BR9506666A patent/BR9506666A/pt not_active Application Discontinuation
- 1995-01-31 DE DE69532109T patent/DE69532109T2/de not_active Expired - Lifetime
-
1996
- 1996-07-30 FI FI963016A patent/FI119476B/sv not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE69532109T2 (de) | 2004-08-26 |
EP0742829A1 (en) | 1996-11-20 |
AU1541595A (en) | 1995-08-15 |
PT742829E (pt) | 2004-04-30 |
ES2210283T3 (es) | 2004-07-01 |
HU222982B1 (hu) | 2004-01-28 |
FI963016A (sv) | 1996-09-25 |
FI963016A0 (sv) | 1996-07-30 |
CA2182315C (en) | 2005-11-15 |
KR970700770A (ko) | 1997-02-12 |
JPH09508276A (ja) | 1997-08-26 |
BR9506666A (pt) | 1997-09-09 |
ATE254178T1 (de) | 2003-11-15 |
AU701384B2 (en) | 1999-01-28 |
HU9602084D0 (en) | 1996-09-30 |
EP0742829B1 (en) | 2003-11-12 |
WO1995020665A1 (en) | 1995-08-03 |
DK0742829T3 (da) | 2004-03-22 |
CZ218296A3 (en) | 1996-10-16 |
HUT75734A (en) | 1997-05-28 |
CA2182315A1 (en) | 1995-08-03 |
PL180091B1 (pl) | 2000-12-29 |
KR100392916B1 (ko) | 2003-10-22 |
CZ285867B6 (cs) | 1999-11-17 |
SK99696A3 (en) | 1997-01-08 |
GB9401795D0 (en) | 1994-03-23 |
NZ278930A (en) | 1998-07-28 |
PL315670A1 (en) | 1996-11-25 |
DE69532109D1 (de) | 2003-12-18 |
JP3731004B2 (ja) | 2006-01-05 |
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