FI110009B - En transformationssystem i Camelina sativa - Google Patents

En transformationssystem i Camelina sativa Download PDF

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Publication number
FI110009B
FI110009B FI20002478A FI20002478A FI110009B FI 110009 B FI110009 B FI 110009B FI 20002478 A FI20002478 A FI 20002478A FI 20002478 A FI20002478 A FI 20002478A FI 110009 B FI110009 B FI 110009B
Authority
FI
Finland
Prior art keywords
camelina
sativa
plant
transformation
transgenic
Prior art date
Application number
FI20002478A
Other languages
English (en)
Finnish (fi)
Other versions
FI20002478A (sv
FI20002478A0 (sv
Inventor
Eija Pehu
Kimmo Koivu
Anne Kanerva
Viktor Kuvshinov
Svetlana Kuvshinova
Original Assignee
Unicrop Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unicrop Ltd filed Critical Unicrop Ltd
Publication of FI20002478A0 publication Critical patent/FI20002478A0/sv
Priority to FI20002478A priority Critical patent/FI110009B/sv
Priority to US10/416,091 priority patent/US20040031076A1/en
Priority to EP01982516A priority patent/EP1334199B1/en
Priority to AU2002214078A priority patent/AU2002214078A1/en
Priority to AT01982516T priority patent/ATE516357T1/de
Priority to CA002427117A priority patent/CA2427117A1/en
Priority to PCT/FI2001/000978 priority patent/WO2002038779A1/en
Publication of FI20002478A publication Critical patent/FI20002478A/sv
Application granted granted Critical
Publication of FI110009B publication Critical patent/FI110009B/sv
Priority to US12/288,791 priority patent/US7910803B2/en
Priority to US12/290,379 priority patent/US20090151023A1/en
Priority to US13/385,329 priority patent/US20120192318A1/en
Priority to US14/173,716 priority patent/US20140223607A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Processing Of Color Television Signals (AREA)

Claims (18)

1. Förfarande för Agrobakterie-förmedlad genetisk transformation, kännetecknat av att den innefattar skeden där a) transplantat frän Camelina sativa skaffas; b) transplantat av Camelina sativa sättas i kontakt med en Agrobaherie-stamkultur, som innehäller atminstone en rekombinant-DNA-konstrukt; c) transformationen tilläts att pägä i ett vävnadskulturmedium, till vilket man valfritt har tillsatt atminstone en hormon; d) transgen vävnad av Camelina sativa utskiljs i ett medium, som innehäller en urskiljande komponent; e) formning av skott induceras (regeneration av skott) frän transgena transplantat i vävnadskulturmedium, som innehäller ätminstone en hormon; och f) skott odlas till hela Camelina sativa -plantor.
2. Förfarande enligt patentkrav 1, kännetecknat av att Agrobakterie-stammen är Agrobacterium tumefaciens.
• · • '·· 3. Förfarande enligt patentkrav 1, kännetecknat av att transplantaten av Camelina . : ’ sativa är ympade med en A. tumefaciens-kultur.
• · · .···. 4. Förfarande enligt patentkrav 1, kännetecknat av att transplantaten av Camelina • t · . . sativa är valda bland hjärtblad, hjärtstam, stam, blad eller andra organ av växten.
,, 5. Förfarande enligt patentkrav 1, kännetecknat av att induktionen av skotten • « · innefattar användning av hormon eller växtfaktor för att ästadkomma en regeneration av skotten. * · t
6. Förfarande enligt patentkrav 1, kännetecknat av att det valfria hormonet är [:. auxin eller cytokinin. • t » · » · 23 1 10009
7. Förfarande enligt patentkrav 1, kännetecknat av att cytokininen är 6-benzylaminopurin (BAP).
8. Förfarande enligt patentkrav 1, kännetecknat av att 6-benzylaminopurin (BAP) koncentrationen är cirka 0.3 -3 mg/1.
9. Förfarande enligt patentkrav 1, kännetecknat av att auxinen är α- naftalenättiksyra (NAA).
10. Förfarande enligt patentkrav 1, kännetecknat av att α-naftalenättiksyra (NAA) koncentrationen är cirka 0.1 - 0.7 mg/1.
11. Förfarande enligt patentkrav 1, kännetecknat av att auxinen är indolyl-3-ättiksyra (IAA).
12. Förfarande enligt patentkrav 1, kännetecknat av att indolyl-3-ättiksyra (IAA) koncentrationen valfritt är under 1.5 mg/1.
13. Förfarande enligt patentkrav 1, kännetecknat av att den urskiljande komponenten är ett antibiotikum.
. . 14. Förfarande enligt patentkrav 1, kännetecknat av att antibiotikumet är ' kanamycin eller hygromycin.
·.: 15. En transgen växt, kännetecknad av att den är Camelina sotiva, som erhälls med * · ': förfarandet enligt patentkrav 1.
• < · : 16. Transgen vävnad, kännetecknad av att den är Camelina sativa, som erhälls med förfarandet enligt patentkrav 1. * f
• · · • · · ,···, 17. Transgena växtceller och cellinjer, kännetecknade av att de är Camelina sativa, * * · • , som erhälls med förfarandet enligt patentkrav 1. • * · • * *
18. Transgent frö, kännetecknat av att det är Camelina sativa, som erhälls med .:. förfarandet enligt patentkravl. » · Ml
FI20002478A 2000-11-13 2000-11-13 En transformationssystem i Camelina sativa FI110009B (sv)

Priority Applications (11)

Application Number Priority Date Filing Date Title
FI20002478A FI110009B (sv) 2000-11-13 2000-11-13 En transformationssystem i Camelina sativa
PCT/FI2001/000978 WO2002038779A1 (en) 2000-11-13 2001-11-12 A transformation system in camelina sativa
EP01982516A EP1334199B1 (en) 2000-11-13 2001-11-12 A transformation system in camelina sativa
AU2002214078A AU2002214078A1 (en) 2000-11-13 2001-11-12 A transformation system in camelina sativa
AT01982516T ATE516357T1 (de) 2000-11-13 2001-11-12 Transformationssystem in camelina sativa
CA002427117A CA2427117A1 (en) 2000-11-13 2001-11-12 A transformation system in camelina sativa
US10/416,091 US20040031076A1 (en) 2000-11-13 2001-11-12 Transformation system in camelina sativa
US12/288,791 US7910803B2 (en) 2000-11-13 2008-10-23 Transformation in Camelina sativa
US12/290,379 US20090151023A1 (en) 2000-11-13 2008-10-30 Transformation system for Camelina sativa
US13/385,329 US20120192318A1 (en) 2000-11-13 2012-02-14 Transformation system for Camelina sativa
US14/173,716 US20140223607A1 (en) 2000-11-13 2014-02-05 Transformation system in camelina sativa

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20002478A FI110009B (sv) 2000-11-13 2000-11-13 En transformationssystem i Camelina sativa
FI20002478 2000-11-13

Publications (3)

Publication Number Publication Date
FI20002478A0 FI20002478A0 (sv) 2000-11-13
FI20002478A FI20002478A (sv) 2002-05-14
FI110009B true FI110009B (sv) 2002-11-15

Family

ID=8559479

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20002478A FI110009B (sv) 2000-11-13 2000-11-13 En transformationssystem i Camelina sativa

Country Status (7)

Country Link
US (4) US20040031076A1 (sv)
EP (1) EP1334199B1 (sv)
AT (1) ATE516357T1 (sv)
AU (1) AU2002214078A1 (sv)
CA (1) CA2427117A1 (sv)
FI (1) FI110009B (sv)
WO (1) WO2002038779A1 (sv)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2271761A4 (en) * 2008-03-21 2011-11-02 Targeted Growth Inc FLORAL SOFTENING METHOD FOR CAMELINA TRANSFORMATION
WO2010147636A1 (en) * 2009-06-15 2010-12-23 Huttenbauer, Samuel, Jr. Herbicide resistant camelina sativa
US20120266268A1 (en) * 2009-09-25 2012-10-18 Washington State University Genetic manipulation of the at-hook domain in plant ahl genes to modulate cell growth
AU2012242991B2 (en) 2011-04-11 2017-03-02 Targeted Growth, Inc. Identification and the use of KRP mutants in plants
AU2013246661B2 (en) 2012-04-12 2018-12-20 Rothamsted Research Ltd Production of omega-3 long chain polyunsaturated fatty acids
US9943095B2 (en) 2012-10-23 2018-04-17 Montana State University Production of high quality durum wheat having increased amylose content
US9862962B2 (en) 2013-03-14 2018-01-09 EG Corp Science, Inc. Identification and use of tomato genes controlling salt/drought tolerance and fruit sweetness
CA3012514C (en) 2013-10-29 2020-09-08 Biotech Institute, Llc Production and use of specialty cannabis with bd/bt genotype and a terpinolene-dominant terpene profile
EP2868748A1 (en) * 2013-10-31 2015-05-06 Locusia Oy A method for protein production in doubled haploid plants
CN104067801B (zh) * 2014-06-17 2016-04-13 长江大学 一种移栽烟草苗动态优势根区的判断方法
WO2015196100A1 (en) * 2014-06-20 2015-12-23 Donald Danforth Plant Science Center Constructs and methods for biosynthesis of galanthamine
WO2017158514A1 (en) 2016-03-15 2017-09-21 National Research Council Of Canada Modulating plant abiotic stress responses using the kanghan gene family
US11859196B2 (en) 2016-03-15 2024-01-02 National Research Council Of Canada Modulating drought tolerance in Brassicaceae using the Kanghan gene family
WO2022093977A1 (en) 2020-10-30 2022-05-05 Fortiphyte, Inc. Pathogen resistance in plants
US11483992B2 (en) 2020-11-16 2022-11-01 Sustainable Oils, Inc. Camelina sativa variety “SO-70”
US11470796B2 (en) 2020-11-16 2022-10-18 Sustainable Oils, Inc. Camelina sativa variety “SO-90”
US11632924B2 (en) * 2020-12-02 2023-04-25 Sustainable Oils, Inc. Camelina sativa variety “SO-110”
US11632923B2 (en) 2020-12-02 2023-04-25 Sustainable Oils, Inc. Camelina sativa variety “SO-100”
US20240093220A1 (en) 2022-09-09 2024-03-21 Friedrich Alexander Universität Erlangen-Nürnberg Plant regulatory elements and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750871A (en) 1986-05-29 1998-05-12 Calgene, Inc. Transformation and foreign gene expression in Brassica species
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
FI104907B (sv) * 1997-09-18 2000-04-28 Univ Helsinki Licensing Agrobacterium-förmedlad transformation av ryps
US6117476A (en) 1999-01-04 2000-09-12 Shaul Eger Healthy food spreads
AU2001268313A1 (en) * 2000-06-07 2001-12-17 North Carolina State University Method of using DNA episomes to suppress gene expression in plants

Also Published As

Publication number Publication date
ATE516357T1 (de) 2011-07-15
US20090151028A1 (en) 2009-06-11
WO2002038779A1 (en) 2002-05-16
AU2002214078A1 (en) 2002-05-21
EP1334199A1 (en) 2003-08-13
FI20002478A (sv) 2002-05-14
US7910803B2 (en) 2011-03-22
CA2427117A1 (en) 2002-05-16
EP1334199B1 (en) 2011-07-13
US20040031076A1 (en) 2004-02-12
US20120192318A1 (en) 2012-07-26
US20140223607A1 (en) 2014-08-07
FI20002478A0 (sv) 2000-11-13

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