FI103413B1 - Förfarande för detektering av spesifikt mRNA och DNA i celler - Google Patents

Förfarande för detektering av spesifikt mRNA och DNA i celler

Info

Publication number
FI103413B1
FI103413B1 FI924242A FI924242A FI103413B1 FI 103413 B1 FI103413 B1 FI 103413B1 FI 924242 A FI924242 A FI 924242A FI 924242 A FI924242 A FI 924242A FI 103413 B1 FI103413 B1 FI 103413B1
Authority
FI
Finland
Prior art keywords
cells
screening
compounds
applicable
specific mrna
Prior art date
Application number
FI924242A
Other languages
English (en)
Finnish (fi)
Other versions
FI924242A0 (fi
FI924242A (fi
FI103413B (sv
Inventor
Michael J Banker
Ralph E Davidson
Dennis A Pereira
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of FI924242A0 publication Critical patent/FI924242A0/fi
Publication of FI924242A publication Critical patent/FI924242A/fi
Application granted granted Critical
Publication of FI103413B1 publication Critical patent/FI103413B1/sv
Publication of FI103413B publication Critical patent/FI103413B/sv

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
FI924242A 1991-09-23 1992-09-22 Förfarande för detektering av spesifikt mRNA och DNA i celler FI103413B (sv)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US76446291A 1991-09-23 1991-09-23

Publications (4)

Publication Number Publication Date
FI924242A0 FI924242A0 (fi) 1992-09-22
FI924242A FI924242A (fi) 1993-03-24
FI103413B1 true FI103413B1 (sv) 1999-06-30
FI103413B FI103413B (sv) 1999-06-30

Family

ID=25070802

Family Applications (1)

Application Number Title Priority Date Filing Date
FI924242A FI103413B (sv) 1991-09-23 1992-09-22 Förfarande för detektering av spesifikt mRNA och DNA i celler

Country Status (10)

Country Link
US (1) US5643730A (sv)
EP (1) EP0534640B1 (sv)
JP (1) JP2703156B2 (sv)
AT (1) ATE143700T1 (sv)
CA (1) CA2078703C (sv)
DE (1) DE69214243T2 (sv)
DK (1) DK0534640T3 (sv)
ES (1) ES2092056T3 (sv)
FI (1) FI103413B (sv)
GR (1) GR3021721T3 (sv)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
AU7640494A (en) * 1993-09-03 1995-03-22 Cellpro, Incorporated Methods for quantifying the number of cells containing a selected nucleic acid sequence in a heterogenous population of cells
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
US6017496A (en) 1995-06-07 2000-01-25 Irori Matrices with memories and uses thereof
WO1997029211A1 (en) 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
US5955269A (en) * 1996-06-20 1999-09-21 Rutgers, The State University Of New Jersey Methods of screening foods for nutraceuticals
EP0956364A1 (en) * 1996-07-26 1999-11-17 Genzyme Corporation Whole cell assay
EP0822261B1 (en) * 1996-07-29 2002-07-03 Academia Sinica Methods for rapid antimicrobial susceptibility testing
US20020018989A1 (en) * 1996-11-07 2002-02-14 Albert Beaufour Method of identifying pharmaceuticals from plant extracts
US6083763A (en) * 1996-12-31 2000-07-04 Genometrix Inc. Multiplexed molecular analysis apparatus and method
NO972006D0 (no) * 1997-04-30 1997-04-30 Forskningsparken I Aas As Ny metode for diagnose av sykdommer
US6849400B1 (en) 1997-07-23 2005-02-01 Gen-Probe Incorporated Methods for detecting and measuring spliced nucleic acids
US6573044B1 (en) 1997-08-07 2003-06-03 The Regents Of The University Of California Methods of using chemical libraries to search for new kinase inhibitors
EP1027607A1 (en) * 1997-10-31 2000-08-16 Sarnoff Corporation Method for enhancing fluorescence
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US6458533B1 (en) 1997-12-19 2002-10-01 High Throughput Genomics, Inc. High throughput assay system for monitoring ESTs
US20030096232A1 (en) * 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6232066B1 (en) 1997-12-19 2001-05-15 Neogen, Inc. High throughput assay system
US20030039967A1 (en) * 1997-12-19 2003-02-27 Kris Richard M. High throughput assay system using mass spectrometry
WO1999034018A1 (en) * 1997-12-24 1999-07-08 The Regents Of The University Of California Methods of using chemical libraries to search for new kinase inhibitors
WO1999050401A1 (fr) * 1998-03-27 1999-10-07 Helix Research Institute Procede pour detecter les changements de l'expression genique par le traitement avec un compose de test
US6448003B1 (en) * 1998-06-10 2002-09-10 Dna Sciences Laboratories, Inc. Genotyping the human phenol sulfotransferbase 2 gene STP2
CA2702148C (en) 1999-01-06 2014-03-04 Genenews Inc. Method of profiling gene expression in a human subject having an infectious disease
US7473528B2 (en) 1999-01-06 2009-01-06 Genenews Inc. Method for the detection of Chagas disease related gene transcripts in blood
GB2355791B (en) 1999-10-28 2004-10-20 Molecular Light Tech Res Ltd Detection of mRNA expression using chemiluminescent labelled probes
US20010046665A1 (en) * 2000-01-21 2001-11-29 Powell Thomas J. Method of identifying the function of a test agent
US20040185464A1 (en) * 2000-09-15 2004-09-23 Kris Richard M. High throughput assay system
JP2003116543A (ja) * 2001-10-10 2003-04-22 Tosoh Corp 薬効と毒性の評価法
US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
GB0412301D0 (en) 2004-06-02 2004-07-07 Diagenic As Product and method
JP4733936B2 (ja) * 2004-07-08 2011-07-27 大陽日酸株式会社 Ｒｎａ追跡方法
JP2022102386A (ja) * 2020-12-25 2022-07-07 国立大学法人旭川医科大学 遺伝子解析用サンプルの製造方法

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358586A (en) * 1980-12-23 1982-11-09 Harvey Rubin Detection and isolation of endorphin mRNA using a synthetic oligodeoxynucleotide
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
CA1254114A (en) * 1984-05-07 1989-05-16 Allied Corporation Displacement polynucleotide assay employing recombination protein and diagnostic kit
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
FI76119C (sv) * 1986-02-27 1988-09-09 Orion Yhtymae Oy Kvantitativ bestämning av nukleinsyramolekyler och reagensförpackning som används vid förfarandet
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
WO1988008038A1 (en) * 1987-04-15 1988-10-20 Yissum Research Development Company Of The Hebrew Method for detecting the expression of bioregulatory genes in microcultures of eukaryotic cells and kit therefor
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
WO1989001050A1 (en) * 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
IT1222509B (it) * 1987-08-17 1990-09-05 Miat Spa Insufflatore per la somministrazione di farmaci sotto forma di polvere predosata in opercoli
EP0326395A2 (en) * 1988-01-29 1989-08-02 City Of Hope Method of detecting and identifying certain viral sequences
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US4999290A (en) * 1988-03-31 1991-03-12 The Board Of Regents, The University Of Texas System Detection of genomic abnormalities with unique aberrant gene transcripts
DE68921918T2 (de) * 1988-05-09 1995-09-07 Univ Temple Verfahren zur Voraussage der Wirksamkeit einer antineoplastichen Behandlung bei einzelnen Patienten.
WO1990001069A1 (en) * 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
JPH04501353A (ja) * 1988-07-26 1992-03-12 ジエネラブス・テクノロジーズ・インコーポレイテツド Ｒｎａ及びｄｎａ増幅法
US5182377A (en) * 1988-09-09 1993-01-26 Hoffmann-La Roche Inc. Probes for detection of human papillomavirus
US4969603A (en) * 1988-11-01 1990-11-13 R. O. Norman Company, Inc. Fluid spray system having a replaceable cartridge
EP0370813A3 (en) * 1988-11-25 1991-06-19 Exemplar Corporation Rapid screening mutagenesis and teratogenesis assay
WO1990009456A1 (de) * 1989-02-16 1990-08-23 Viktor Balazs Malignitätstest (krebs-test) mit verwendung von der polymerasekettenreaktion
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
IE81145B1 (en) * 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
PT94001A (pt) * 1989-05-11 1991-02-08 Xytronyx Inc Processo de deteccao da presenca de uma infeccao por pneumocystis carinii
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
WO1991005064A1 (en) * 1989-10-02 1991-04-18 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce A sensitive method for measurement of chimeric transcripts of dna containing translocations
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
JP3001919B2 (ja) * 1990-01-12 2000-01-24 寳酒造株式会社 蛍光標識ｄｎａの調製方法及びキット

Also Published As

Publication number Publication date
FI924242A0 (fi) 1992-09-22
CA2078703A1 (en) 1993-03-24
GR3021721T3 (en) 1997-02-28
DE69214243T2 (de) 1997-02-06
DE69214243D1 (de) 1996-11-07
EP0534640B1 (en) 1996-10-02
ES2092056T3 (es) 1996-11-16
FI924242A (fi) 1993-03-24
DK0534640T3 (sv) 1997-03-17
CA2078703C (en) 2000-10-17
ATE143700T1 (de) 1996-10-15
FI103413B (sv) 1999-06-30
JP2703156B2 (ja) 1998-01-26
US5643730A (en) 1997-07-01
JPH05192199A (ja) 1993-08-03
EP0534640A1 (en) 1993-03-31

Similar Documents

Publication Publication Date Title
FI103413B1 (sv) Förfarande för detektering av spesifikt mRNA och DNA i celler
Chase et al. Biogenesis of mitochondria duringXenopus laevis development
DK0919565T4 (da) Mærkede oligonukleotider
ATE87032T1 (de) Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
Tessman UV-induced mutagenesis of phage S13 can occur in the absence of the RecA and UmuC proteins of Escherichia coli.
Achmann et al. Last–male sperm precedence in the bushcricket Poecilimon veluchianus (Orthoptera, Tettigonioidea) demonstrated by DNA fingerprinting
Henderson et al. The mechanism of interferon induction by UV-irradiated reovirus
Doudney et al. Acriflavine modification of nucleic acid formation, mutation induction and survival in ultraviolet light exposed bacteria
Kaneko et al. Glial-specific cAMP response of the glial fibrillary acidic protein gene cell lines.
Petronijevic et al. Gene activity and the development of early parasitic stages of nematodes
Buckel et al. Lack of accumulation of unusual guanosine nucleotides upon amino acid starvation of two eukaryotic organisms
Van Baalen Mutagenesis and genetic recombination
Okaichi et al. DNA damage and its repair in Dictyostelium discoideum irradiated by health lamp light (UV‐B)
Crkvenjakov et al. The effect of 5-azacytidine on development, nucleic acid and protein metabolism in sea urchin embryos
DE19544374C1 (de) Plasmid und damit transformierter Mikroorganismus für ein Verfahren zur luminometrischen Bestimmung der mutagenen und/oder karzinogenen Potenz eines Agens
Croissant et al. Identification and genomic cloning of CMHC1: A unique myosin heavy chain expressed exclusively in the developing chicken heart
NZ531656A (en) Methods of detection of conformational change in a nucleic acid duplex by treatment with oxidising or reactive agent as a result of exposure to environmental or chemical conditions
Ahnström et al. Chemically induced somatic mutations in Arabidopsis
Shiraishi Differential reactivity in mammalian chromosomes: I. Relation between special segments and Late-labeling patterns of DNA synthesis in Human X-chromosomes
GB1563284A (en) Production of mutant of aujeszky's virus
Benndorf et al. Chemicals and heat generate different protein patterns in Acinetobacter calcoaceticus
Reddy et al. Identification and characterization of a polyadenylated small RNA (s-poly A+ RNA) in dinoflagellates
RU2002263C1 (ru) Способ получени препарата дл токсикологических исследований объектов внешней среды
Day III The Photobiology of the DNA Viruses λ, T4, and φ X174
CN101300347A (zh) 通过轻度的低温促进基因表达的序列