ES8802074A1 - Un metodo para preparar un vector de expresion que tiene un sistema de replicacion funcional en un microorganismo hospedante - Google Patents
Un metodo para preparar un vector de expresion que tiene un sistema de replicacion funcional en un microorganismo hospedanteInfo
- Publication number
- ES8802074A1 ES8802074A1 ES554630A ES554630A ES8802074A1 ES 8802074 A1 ES8802074 A1 ES 8802074A1 ES 554630 A ES554630 A ES 554630A ES 554630 A ES554630 A ES 554630A ES 8802074 A1 ES8802074 A1 ES 8802074A1
- Authority
- ES
- Spain
- Prior art keywords
- polypeptides
- growth factor
- factor activity
- nucleic acid
- acid sequences
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 239000003102 growth factor Substances 0.000 title abstract 3
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 title 1
- 102000018386 EGF Family of Proteins Human genes 0.000 abstract 2
- 108010066486 EGF Family of Proteins Proteins 0.000 abstract 2
- 239000000654 additive Substances 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- 150000001945 cysteines Chemical class 0.000 abstract 1
- 239000003226 mitogen Substances 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
- 230000029663 wound healing Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
EL VECTOR DE EXPRESION QUE TIENE UN SISTEMA DE REPLICACION FUNCIONAL EN UN MICROORGANISMO HOSPEDANTE, SEÑALES REGULADORAS DE INICIACION Y TERMINACION DE LA TRANSCRIPCION Y DE LA TRADUCCION FUNCIONALES EN DICHO HOSPEDANTE; Y UNA SECUENCIA DE ADN ENTRE LAS SEÑALES, QUE CODIFICA UN POLIPEPTIDO (I), CON RADICALES INDICADOS EN LA MEMORIA; SE OBTIENE MEDIANTE LAS ETAPAS: A) AISLAR EL ARNM DE UN VIRUS DE VIRUELA; B) SOMETERLE A TRANSCRIPCION INVERSA PARA PRODUCIR ADN MONOCATENARIO; C) A PARTIR DE ESTE, PREPARAR ADN BICATENARIO; D) AISLAR LA SECUENCIA DE ADN BICATENARIO QUE CODIFICA EL POLIPEPTIDO; Y E) INSERTAR ESTA SECUENCIA ENTRE LAS SEÑALES DE INICIACION Y TERMINACION DEL VECTOR. EL POLIPEPTIDO ACTUA COMO MITOGENO Y ES APLICABLE COMO REACTIVO EN LA DETECCION DE FACTORES DE CRECIMIENTO Y PARA FAVORECER LA EPITELIZACION Y CURACION DE QUEMADURAS Y HERIDAS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US66652084A | 1984-10-30 | 1984-10-30 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES554630A0 ES554630A0 (es) | 1988-03-16 |
ES8802074A1 true ES8802074A1 (es) | 1988-03-16 |
Family
ID=24674411
Family Applications (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES548316A Expired ES8702925A1 (es) | 1984-10-30 | 1985-10-29 | Un procedimiento para preparar un polipeptido que tiene ac- tividad de factor de crecimiento |
ES554628A Expired ES8802061A1 (es) | 1984-10-30 | 1986-04-30 | Un metodo para preparar una secuencia de adn que codifica un polipeptido que tiene actividad de factor de crecimiento |
ES554629A Expired ES8802185A1 (es) | 1984-10-30 | 1986-04-30 | Un metodo para preparar un anticuerpo util para la deteccion de un factor de crecimiento epidermico |
ES554630A Expired ES8802074A1 (es) | 1984-10-30 | 1986-04-30 | Un metodo para preparar un vector de expresion que tiene un sistema de replicacion funcional en un microorganismo hospedante |
ES557145A Expired ES8707741A1 (es) | 1984-10-30 | 1986-10-24 | Un procedimiento para prepara un polipeptido que tiene actividad de factor de crecimiento |
ES557710A Expired ES8801379A1 (es) | 1984-10-30 | 1987-09-01 | Un metodo para preparar una secuencia de adn que codifica un polipeptido que tiene actividad de factor de crecimiento |
ES557708A Expired ES8801378A1 (es) | 1984-10-30 | 1987-09-01 | Un metodo para preparar un vector de expresion que tiene un sistema de replicacion funcional en un microorganismo hospedante |
Family Applications Before (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES548316A Expired ES8702925A1 (es) | 1984-10-30 | 1985-10-29 | Un procedimiento para preparar un polipeptido que tiene ac- tividad de factor de crecimiento |
ES554628A Expired ES8802061A1 (es) | 1984-10-30 | 1986-04-30 | Un metodo para preparar una secuencia de adn que codifica un polipeptido que tiene actividad de factor de crecimiento |
ES554629A Expired ES8802185A1 (es) | 1984-10-30 | 1986-04-30 | Un metodo para preparar un anticuerpo util para la deteccion de un factor de crecimiento epidermico |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES557145A Expired ES8707741A1 (es) | 1984-10-30 | 1986-10-24 | Un procedimiento para prepara un polipeptido que tiene actividad de factor de crecimiento |
ES557710A Expired ES8801379A1 (es) | 1984-10-30 | 1987-09-01 | Un metodo para preparar una secuencia de adn que codifica un polipeptido que tiene actividad de factor de crecimiento |
ES557708A Expired ES8801378A1 (es) | 1984-10-30 | 1987-09-01 | Un metodo para preparar un vector de expresion que tiene un sistema de replicacion funcional en un microorganismo hospedante |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0199769A4 (es) |
JP (1) | JPS62501071A (es) |
AU (2) | AU600433B2 (es) |
ES (7) | ES8702925A1 (es) |
FI (1) | FI862734A0 (es) |
GR (1) | GR852610B (es) |
IL (1) | IL76866A0 (es) |
NO (1) | NO862613D0 (es) |
NZ (1) | NZ213984A (es) |
PH (2) | PH24603A (es) |
PT (1) | PT81408B (es) |
WO (1) | WO1986002650A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5240912A (en) * | 1983-05-09 | 1993-08-31 | Todaro George J | Transforming growth factor (TGF) peptides |
US4863899A (en) * | 1983-05-09 | 1989-09-05 | Todaro George J | Biologically active polypeptides |
EP0228036A3 (en) * | 1985-12-18 | 1988-09-07 | Microgenesys, Inc. | Method for producing selected polypeptides in virally infected insect cells and polypeptides isolated therefrom |
IL89673A0 (en) * | 1988-03-24 | 1989-09-28 | Oncogen | Novel polypeptides having growth factor activity and nucleic acid sequences encoding the polypeptides |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE38892B1 (en) * | 1973-03-28 | 1978-06-21 | Ici Ltd | Pharmaceutical compositions |
US4366246A (en) * | 1977-11-08 | 1982-12-28 | Genentech, Inc. | Method for microbial polypeptide expression |
US4230691A (en) * | 1978-05-23 | 1980-10-28 | The Massachusetts General Hospital | Nerve growth factor antibody and process |
IE53166B1 (en) * | 1980-08-05 | 1988-08-03 | Searle & Co | Synthetic urogastrone gene,corresponding plasmid recombinants,transformed cells,production thereof and urogastrone expression |
JPH064673B2 (ja) * | 1980-11-10 | 1994-01-19 | ゲネンテツク・インコ−ポレ−テツド | ハイブリド型ヒト白血球インタフエロン |
WO1985002198A1 (en) * | 1983-11-01 | 1985-05-23 | Amgen | Microbial expression of type i transforming growth factor, polypeptide analogs thereof and hybrid egf/tgf polypeptides |
GB8507666D0 (en) * | 1985-03-25 | 1985-05-01 | Wellcome Found | Epidermal growth factor production |
-
1985
- 1985-10-23 JP JP60504725A patent/JPS62501071A/ja active Pending
- 1985-10-23 WO PCT/US1985/002103 patent/WO1986002650A1/en not_active Application Discontinuation
- 1985-10-23 AU AU48687/85A patent/AU600433B2/en not_active Ceased
- 1985-10-23 EP EP19850905376 patent/EP0199769A4/en not_active Withdrawn
- 1985-10-28 IL IL76866A patent/IL76866A0/xx unknown
- 1985-10-29 GR GR852610A patent/GR852610B/el unknown
- 1985-10-29 NZ NZ213984A patent/NZ213984A/en unknown
- 1985-10-29 ES ES548316A patent/ES8702925A1/es not_active Expired
- 1985-10-30 PH PH32995A patent/PH24603A/en unknown
- 1985-10-30 PT PT81408A patent/PT81408B/pt not_active IP Right Cessation
-
1986
- 1986-04-30 ES ES554628A patent/ES8802061A1/es not_active Expired
- 1986-04-30 ES ES554629A patent/ES8802185A1/es not_active Expired
- 1986-04-30 ES ES554630A patent/ES8802074A1/es not_active Expired
- 1986-06-26 FI FI862734A patent/FI862734A0/fi not_active IP Right Cessation
- 1986-06-27 NO NO1986862613A patent/NO862613D0/no unknown
- 1986-10-24 ES ES557145A patent/ES8707741A1/es not_active Expired
-
1987
- 1987-09-01 ES ES557710A patent/ES8801379A1/es not_active Expired
- 1987-09-01 ES ES557708A patent/ES8801378A1/es not_active Expired
-
1989
- 1989-03-27 PH PH38379A patent/PH25794A/en unknown
-
1990
- 1990-11-12 AU AU66551/90A patent/AU633377B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
PT81408A (en) | 1985-11-01 |
ES8802061A1 (es) | 1988-03-16 |
NO862613L (no) | 1986-06-27 |
ES557708A0 (es) | 1988-01-01 |
PH24603A (en) | 1990-08-17 |
AU600433B2 (en) | 1990-08-16 |
NZ213984A (en) | 1991-05-28 |
WO1986002650A1 (en) | 1986-05-09 |
AU4868785A (en) | 1986-05-15 |
AU633377B2 (en) | 1993-01-28 |
NO862613D0 (no) | 1986-06-27 |
ES557145A0 (es) | 1987-08-16 |
AU6655190A (en) | 1991-01-24 |
ES8801378A1 (es) | 1988-01-01 |
ES554629A0 (es) | 1988-04-01 |
FI862734A (fi) | 1986-06-26 |
PT81408B (pt) | 1987-11-11 |
ES8802185A1 (es) | 1988-04-01 |
FI862734A0 (fi) | 1986-06-26 |
JPS62501071A (ja) | 1987-04-30 |
PH25794A (es) | 1991-11-05 |
ES557710A0 (es) | 1988-01-01 |
ES8801379A1 (es) | 1988-01-01 |
EP0199769A1 (en) | 1986-11-05 |
GR852610B (es) | 1986-03-04 |
IL76866A0 (en) | 1986-02-28 |
ES554630A0 (es) | 1988-03-16 |
EP0199769A4 (en) | 1989-11-29 |
ES8707741A1 (es) | 1987-08-16 |
ES548316A0 (es) | 1987-01-16 |
ES554628A0 (es) | 1988-03-16 |
ES8702925A1 (es) | 1987-01-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970401 |