ES8802235A1 - Procedimiento para preparar un polipeptido - Google Patents

Procedimiento para preparar un polipeptido

Info

Publication number
ES8802235A1
ES8802235A1 ES543257A ES543257A ES8802235A1 ES 8802235 A1 ES8802235 A1 ES 8802235A1 ES 543257 A ES543257 A ES 543257A ES 543257 A ES543257 A ES 543257A ES 8802235 A1 ES8802235 A1 ES 8802235A1
Authority
ES
Spain
Prior art keywords
properties
acid sequence
amino acid
human beta
beta interferon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES543257A
Other languages
English (en)
Other versions
ES543257A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GD Searle LLC
Original Assignee
GD Searle LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GD Searle LLC filed Critical GD Searle LLC
Publication of ES8802235A1 publication Critical patent/ES8802235A1/es
Publication of ES543257A0 publication Critical patent/ES543257A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/811—Interferon
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/14—Lymphokine; related peptides
    • Y10S930/142—Interferon

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL POLIPEPTIDO DE FORMULA: A-R1-B-R22-C, DONDE A ES LA SECUENCIA 1-16, B ID. 18-31, C ID. 53-155 DE AMINOACIDOS DEL INTERFERON BETA HUMANO, R1 CISTEINA, SERINA, ALAMINA Y R2-22 AMINOACIDOS DE ORIGEN NATURAL, DE LOS CUALES DEL R6-18 SON DESDE 1 A 13 AMINOACIDOS SECUENCIALES 32 A 52 DE UN INTERFERON ALFA, POR EJEMPLO DE ORIGEN HUMANO Y ADEMAS EL POLIPEPTIDO COMPRENDE SECUENCIAS DE AMINOACIDOS, TIPO IFNX416; SE OBTIENE: A) SUBCLONANDO UN GEN HUMANO; B) INTRODUCIENDOLO POR <MUTAGENESIS DIRIGIDA POR PUNTA>; C) SEPARANDO EL DNA SUPERHELICOIDAL RESULTANTE; D) SEPARANDO EL DNA MUTANTE PORTADOR DE UN PUNTO XHO I UNICO; E) RECLONANDO EL GEN COMPLETO CON UN PUNTO XHO I; Y F) CLONANDO EL FRAGMENTO DE DNA SINTETICO EN LOS PUNTOS CLA I-XHO I DEL PLASMIDO, UTILIZANDO EN ESTE PROCESO AL E. COLI JM101.
ES543257A 1984-05-17 1985-05-17 Procedimiento para preparar un polipeptido Expired ES8802235A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB848412564A GB8412564D0 (en) 1984-05-17 1984-05-17 Structure and properties

Publications (2)

Publication Number Publication Date
ES8802235A1 true ES8802235A1 (es) 1988-04-16
ES543257A0 ES543257A0 (es) 1988-04-16

Family

ID=10561073

Family Applications (1)

Application Number Title Priority Date Filing Date
ES543257A Expired ES8802235A1 (es) 1984-05-17 1985-05-17 Procedimiento para preparar un polipeptido

Country Status (7)

Country Link
US (2) US4769233A (es)
EP (1) EP0163993A3 (es)
JP (1) JPS6156200A (es)
AU (1) AU589560B2 (es)
ES (1) ES8802235A1 (es)
GB (1) GB8412564D0 (es)
ZA (1) ZA853750B (es)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0205404B1 (en) * 1985-06-11 1992-07-15 Ciba-Geigy Ag Hybrid interferons
EP0237019A3 (en) * 1986-03-14 1988-03-09 Toray Industries, Inc. Interferon conjugate and production thereof using recombinant gene
DE3712564A1 (de) * 1987-04-14 1988-11-24 Bioferon Biochem Substanz Verfahren zur konstruktion einer animalen zellinie fuer die herstellung von humanem interferon-beta
US4904584A (en) * 1987-12-23 1990-02-27 Genetics Institute, Inc. Site-specific homogeneous modification of polypeptides
US6682896B1 (en) 1988-10-14 2004-01-27 Schering Aktiengesellschaft Peptides representing epitopic sites on r-IFN-β, antibodies thereto and uses thereof
JP2717319B2 (ja) * 1988-10-14 1998-02-18 ベルレックス、ラボラトリーズ、インコーポレイテッド Ｒ―ｉｆｎ―ベータエピトープ部位を代表するペプチド、それに対する抗体、およびその用途
IL89662A (en) * 1989-03-19 1997-11-20 Interpharm Lab Ltd Kiryat Weiz Pharmaceutical compositions comprising interferon-beta
US5545723A (en) * 1994-03-15 1996-08-13 Biogen Inc. Muteins of IFN-β
DE19717864C2 (de) 1997-04-23 2001-05-17 Fraunhofer Ges Forschung Humanes rekombinantes Interferon-beta, Verfahren zu seiner Herstellung und seine Verwendung
US6685933B1 (en) 1998-07-28 2004-02-03 The United States Of America As Represented By The Department Of Health And Human Services Interferon α hybrids
BR9915548A (pt) * 1998-10-16 2001-08-14 Biogen Inc Proteìnas de fusão de interferon-beta e usos
DE69930015T2 (de) 1998-10-16 2006-10-12 Biogen Idec Ma Inc., Cambridge Polymerkonjugate von interferon-beta-1a und deren verwendungen
US6514729B1 (en) 1999-05-12 2003-02-04 Xencor, Inc. Recombinant interferon-beta muteins
US7144574B2 (en) * 1999-08-27 2006-12-05 Maxygen Aps Interferon β variants and conjugates
US6531122B1 (en) * 1999-08-27 2003-03-11 Maxygen Aps Interferon-β variants and conjugates
US7431921B2 (en) * 2000-04-14 2008-10-07 Maxygen Aps Interferon beta-like molecules
US20020169290A1 (en) * 2000-11-02 2002-11-14 Claus Bornaes New multimeric interferon beta polypeptides
ATE432288T1 (de) 2001-02-27 2009-06-15 Maxygen Aps Neue interferon-beta-ähnliche moleküle
US6887462B2 (en) 2001-04-09 2005-05-03 Chiron Corporation HSA-free formulations of interferon-beta
DE60332358D1 (de) * 2002-09-09 2010-06-10 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
GB2429207A (en) 2004-02-02 2007-02-21 Ambrx Inc Modified human interferon polypeptides and their uses
US20070179113A1 (en) * 2005-05-19 2007-08-02 Schering Aktiengesellachaft GM-CSF gene therapy for Crohn's disease using an improved regulated expression system
US20080076729A1 (en) * 2005-05-19 2008-03-27 Schering Aktiengesellachaft Interferon-beta gene therapy using an improved, regulated expression system
JP2008545639A (ja) * 2005-05-19 2008-12-18 バイエル・シェーリング・ファルマ・アクチェンゲゼルシャフト 改善された調節発現系を用いる疾患の治療
DOP2006000117A (es) * 2005-05-19 2007-11-30 Schering Ag Terapia génica de interferon-beta usando un sistema mejorado de expresión regulada
US20080038224A1 (en) * 2006-03-28 2008-02-14 Thierry Guyon Modified interferon-beta (IFN-beta) polypeptides
WO2008021412A2 (en) * 2006-08-14 2008-02-21 Biorexis Pharmaceutical Corporation Interferon beta and transferrin fusion proteins
WO2008125222A2 (en) * 2007-04-11 2008-10-23 Bayer Schering Pharma Aktiengesellschaft New modulation molecules for an improved regulated expression system
CN103965347B (zh) * 2007-05-02 2017-07-18 Ambrx公司 经修饰干扰素β多肽和其用途
US7625555B2 (en) 2007-06-18 2009-12-01 Novagen Holding Corporation Recombinant human interferon-like proteins
US8273561B2 (en) * 2007-10-05 2012-09-25 Nuron Biotech, Inc. High pressure treatment of aggregated interferons
AU2008326324B9 (en) 2007-11-20 2012-11-15 Ambrx, Inc. Modified insulin polypeptides and their uses
BR112013003522B1 (pt) 2010-08-17 2021-05-25 Ambrx, Inc. polipeptídeos relaxina modificados compreendendo um aminoácido não codificado naturalmente, seu método de preparação e seu uso, bem como ácido nucleico e célula hospedeira

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0042246B1 (en) * 1980-06-12 1987-03-18 The Cancer Institute Of Japanese Foundation For Cancer Research Plasmid
IT1167610B (it) * 1982-01-19 1987-05-13 Cetus Corp Interfreron ibrido multiclasse, composizione farmaceutica che lo contiene e procedimento di produzione
US4588585A (en) * 1982-10-19 1986-05-13 Cetus Corporation Human recombinant cysteine depleted interferon-β muteins
FI82266C (fi) * 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
GB8317880D0 (en) * 1983-07-01 1983-08-03 Searle & Co Structure and synthesis of interferons
GB8334102D0 (en) * 1983-12-21 1984-02-01 Searle & Co Interferons with cysteine pattern
EP0146903A3 (en) * 1983-12-19 1987-07-22 Schering Corporation Production of a vector encoding a novel hybrid interferon species
ATE47864T1 (de) * 1984-08-27 1989-11-15 Genentech Inc Verschiedenartige familie von menschlichen leukozyten-interferonen, zusammensetzungen, die diese enthalten, verfahren zu ihrer herstellung, sowie dna und transfektierte wirte hierfuer.

Also Published As

Publication number Publication date
US4914033A (en) 1990-04-03
EP0163993A3 (en) 1988-03-16
AU4256185A (en) 1985-11-28
EP0163993A2 (en) 1985-12-11
ZA853750B (en) 1986-07-30
AU589560B2 (en) 1989-10-19
GB8412564D0 (en) 1984-06-20
JPS6156200A (ja) 1986-03-20
ES543257A0 (es) 1988-04-16
US4769233A (en) 1988-09-06

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Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960506