ES8801707A1 - Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable - Google Patents

Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable

Info

Publication number
ES8801707A1
ES8801707A1 ES557479A ES557479A ES8801707A1 ES 8801707 A1 ES8801707 A1 ES 8801707A1 ES 557479 A ES557479 A ES 557479A ES 557479 A ES557479 A ES 557479A ES 8801707 A1 ES8801707 A1 ES 8801707A1
Authority
ES
Spain
Prior art keywords
preparation
modified enzyme
lysine
amino acid
place
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES557479A
Other languages
English (en)
Other versions
ES557479A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858503358A external-priority patent/GB8503358D0/en
Priority claimed from GB868600979A external-priority patent/GB8600979D0/en
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Publication of ES557479A0 publication Critical patent/ES557479A0/es
Publication of ES8801707A1 publication Critical patent/ES8801707A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PROCEDIMIENTO PARA OBTENER UNA CELULA HUESPED TRANSFORMADA CON UN VECTOR DE EXPRESION REPLICABLE TAL COMO UN PLASMIDO O UN BACTERIOFOGO DERIVADO DE PRSU-B-GLOBINA, CAPAZ DE EXPRESAR UNA SECUENCIA DE ADN QUE CODIFICA UN ACTIVADOR DE PLASMINOGENO DE TIPO TEJIDO (T-PA) MODIFICADO, QUE CONTIENE UN AMINOACIDO SIN CADENA LATERAL CARGADA, PREFERENTE/ISOLEMINA, EN LUGAR DE LISINA EN LA POSICION 277. COMPRENDE: A) PREPARACION DE ADN CODIFICANTE DE T-PA MODIFICADO, PREFERENTEMENTE MEDIANTE LA UTILIZACION DE UN OLIGODESOXIRRIBONUCLEOTIDO COMPLEMENTARIO DEL ADNC DEL T-PA QUE SE EMPAREJE MAL EN LA REGION 1018-1020, DE MODO QUE EL TRIPLETE DEL OLIGODESOXIRRIBONUCLEOTIDO EN LA REGION DE MAL EMPAREJAMIENTO, CORRESPONDA A UN CODON QUE CODIFIQUE ISOLEMINA; B) PREPARACION DE UN VECTOR DE EXPRESION REPLICABLE A PARTIR DE UN PLASMIDO O UN BACTERIOFOGO COMO PRSU-B-GLOBINA QUE CONTIENE EL ADNC DE T-PA MODIFICADO; C) TRANSFORMACION DE UNA CELULA HUESPED CON EL VALOR DE EXPRESION REPLICABLE; D) RECUPERACION DEL T-PA MODIFICADO. DE APLICACION EN EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
ES557479A 1985-02-09 1987-03-31 Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable Expired ES8801707A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB858503358A GB8503358D0 (en) 1985-02-09 1985-02-09 Compounds
GB868600979A GB8600979D0 (en) 1986-01-16 1986-01-16 Compounds

Publications (2)

Publication Number Publication Date
ES557479A0 ES557479A0 (es) 1988-02-16
ES8801707A1 true ES8801707A1 (es) 1988-02-16

Family

ID=26288793

Family Applications (4)

Application Number Title Priority Date Filing Date
ES551770A Expired ES8706724A1 (es) 1985-02-09 1986-02-07 Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.
ES557478A Expired ES8801706A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un vector de expresion replicable
ES557479A Expired ES8801707A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable
ES557477A Expired ES8800354A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un polimero de adn

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES551770A Expired ES8706724A1 (es) 1985-02-09 1986-02-07 Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.
ES557478A Expired ES8801706A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un vector de expresion replicable

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES557477A Expired ES8800354A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un polimero de adn

Country Status (6)

Country Link
EP (1) EP0201153A3 (es)
AU (1) AU5330286A (es)
DK (1) DK62586A (es)
ES (4) ES8706724A1 (es)
GR (1) GR860364B (es)
PT (1) PT81999B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661469A (en) * 1984-08-08 1987-04-28 Survival Technology, Inc. t-PA composition capable of being absorbed into the blood stream and method of administration
EP0191814B1 (en) * 1984-08-08 1992-11-04 Survival Technology, Inc. Protein absorption enhancing agents
US5714372A (en) * 1985-04-22 1998-02-03 Genentech, Inc. Tissue plasminogen activator variants
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
PT82429B (pt) * 1985-04-22 1988-03-03 Genentech Inc Novos mutantes do activador de plasmionogenio de tecido humano
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
ES2031826T3 (es) * 1985-12-23 1993-01-01 Chiron Corporation Procedimiento para producir nuevos peptidos activadores del plasminogeno.
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
GB8619098D0 (en) * 1986-08-05 1986-09-17 Wellcome Found Combination
DK323587D0 (da) * 1987-06-25 1987-06-25 Novo Industri As Protein
WO1988005081A2 (en) * 1986-12-30 1988-07-14 Cetus Corporation Novel plasminogen activator
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5580560A (en) * 1989-11-13 1996-12-03 Novo Nordisk A/S Modified factor VII/VIIa
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
EP0643772B1 (en) * 1992-06-03 1997-07-23 Genentech, Inc. Tissue plasminogen activator glycosylation variants with improved therapeutic properties
ES2066697B1 (es) * 1992-12-29 1995-08-16 Azkoyen Ind Sa Perfeccionamientos introducidos en columnas expendedoras automaticas de productos.
WO1997033984A1 (en) * 1996-03-12 1997-09-18 Novo Nordisk A/S Novel achromobacter lyticus protease variants
EP0907746B1 (en) 1996-04-05 2008-02-20 Novartis Vaccines and Diagnostics, Inc. Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
PT82429B (pt) * 1985-04-22 1988-03-03 Genentech Inc Novos mutantes do activador de plasmionogenio de tecido humano

Also Published As

Publication number Publication date
DK62586A (da) 1986-08-10
ES557479A0 (es) 1988-02-16
ES8706724A1 (es) 1987-07-01
ES8801706A1 (es) 1988-02-16
ES8800354A1 (es) 1987-10-16
GR860364B (en) 1986-06-10
PT81999A (en) 1986-03-01
EP0201153A3 (en) 1987-10-07
PT81999B (en) 1987-12-22
EP0201153A2 (en) 1986-11-12
ES551770A0 (es) 1987-07-01
AU5330286A (en) 1986-08-14
ES557478A0 (es) 1988-02-16
DK62586D0 (da) 1986-02-07
ES557477A0 (es) 1987-10-16

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