ES8706724A1 - Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado. - Google Patents

Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.

Info

Publication number
ES8706724A1
ES8706724A1 ES551770A ES551770A ES8706724A1 ES 8706724 A1 ES8706724 A1 ES 8706724A1 ES 551770 A ES551770 A ES 551770A ES 551770 A ES551770 A ES 551770A ES 8706724 A1 ES8706724 A1 ES 8706724A1
Authority
ES
Spain
Prior art keywords
preparation
modified enzyme
lysine
amino acid
place
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551770A
Other languages
English (en)
Other versions
ES551770A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858503358A external-priority patent/GB8503358D0/en
Priority claimed from GB868600979A external-priority patent/GB8600979D0/en
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Publication of ES8706724A1 publication Critical patent/ES8706724A1/es
Publication of ES551770A0 publication Critical patent/ES551770A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

METODO DE PREPARAR ACTIVADOR DE PLASMINOGENO DE TIPO TEJIDO MODIFICADO. CONSISTE EN: A) EXPRESAR ADN CODIFICANTE DEL ACTIVADOR DE PLASMINOGENO DEL TIPO TEJIDO MODIFICADO EN UNA CELULA HUESPED RECOMBINANTE BAJO CONDICIONES QUE PERMITAN LA EXPRESION DEL POLIMERO DE ADN, TALES COMO EN PRESENCIA DE UN MEDIO NUTRIENTE, A TEMPERATURA COMPRENDIDA ENTRE LA AMBIENTE Y 45GC, HASTA QUE POR LO MENOS UNA CANTIDAD DETECTABLE DEL PRODUCTO DE EXPRESION PUEDA SER RECUPERADA DLE MEDIO; Y B) RECUPERAR EL PORDUCTO ACTIVADO DE PLASMINOGENO DE TIPO TEJIDO MODIFICADO MEDIANTE AISLAMIENTO DEL PRODUCTO DEL MEDIO NUTRIENTE, SEGUIDO DE LAS DE LA CELULA EFECTUADA FISICA, QUIMICA O ENZIMATICAMENTE. TIENE APLICACIONES FARMACEUTICAS EN ENFERMEDADES TROMBOTICAS.
ES551770A 1985-02-09 1986-02-07 Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado. Expired ES8706724A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB858503358A GB8503358D0 (en) 1985-02-09 1985-02-09 Compounds
GB868600979A GB8600979D0 (en) 1986-01-16 1986-01-16 Compounds

Publications (2)

Publication Number Publication Date
ES8706724A1 true ES8706724A1 (es) 1987-07-01
ES551770A0 ES551770A0 (es) 1987-07-01

Family

ID=26288793

Family Applications (4)

Application Number Title Priority Date Filing Date
ES551770A Expired ES8706724A1 (es) 1985-02-09 1986-02-07 Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.
ES557478A Expired ES8801706A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un vector de expresion replicable
ES557477A Expired ES8800354A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un polimero de adn
ES557479A Expired ES8801707A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES557478A Expired ES8801706A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un vector de expresion replicable
ES557477A Expired ES8800354A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un polimero de adn
ES557479A Expired ES8801707A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable

Country Status (6)

Country Link
EP (1) EP0201153A3 (es)
AU (1) AU5330286A (es)
DK (1) DK62586A (es)
ES (4) ES8706724A1 (es)
GR (1) GR860364B (es)
PT (1) PT81999B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3586795T2 (de) * 1984-08-08 1993-05-19 Survival Technology Absorptionsverbesserer fuer proteine.
US4661469A (en) * 1984-08-08 1987-04-28 Survival Technology, Inc. t-PA composition capable of being absorbed into the blood stream and method of administration
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
GR861037B (en) * 1985-04-22 1986-07-16 Genentech Inc Novel human tissue plasminogen activator mutants
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
US5714372A (en) * 1985-04-22 1998-02-03 Genentech, Inc. Tissue plasminogen activator variants
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
DE3684680D1 (de) * 1985-12-23 1992-05-07 Chiron Corp Peptidplasminogenaktivatoren.
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
AU7090887A (en) * 1986-04-02 1987-10-08 Beecham Group Plc Plasminogen activators modified in first kringle domain
GB8619098D0 (en) * 1986-08-05 1986-09-17 Wellcome Found Combination
DK323587D0 (da) * 1987-06-25 1987-06-25 Novo Industri As Protein
WO1988005081A2 (en) * 1986-12-30 1988-07-14 Cetus Corporation Novel plasminogen activator
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5580560A (en) * 1989-11-13 1996-12-03 Novo Nordisk A/S Modified factor VII/VIIa
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
DK0643772T3 (da) * 1992-06-03 1998-02-02 Genentech Inc Glycosyleringsvarianter af vævsplasminogenaktivator med forbedrede terapeutiske egenskaber
ES2066697B1 (es) * 1992-12-29 1995-08-16 Azkoyen Ind Sa Perfeccionamientos introducidos en columnas expendedoras automaticas de productos.
AU2091397A (en) * 1996-03-12 1997-10-01 Novo Nordisk A/S Novel achromobacter lyticus protease variants
ATE386811T1 (de) 1996-04-05 2008-03-15 Novartis Vaccines & Diagnostic Alphaviren-vektors mit eine reduzierte inhibierung der synthese von zellmakromolekülen
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR241654A1 (es) * 1982-05-05 1992-10-30 Genentech Inc Procedimiento para producir activador de plasminogeno de tejido humano.
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
GR861037B (en) * 1985-04-22 1986-07-16 Genentech Inc Novel human tissue plasminogen activator mutants

Also Published As

Publication number Publication date
PT81999A (en) 1986-03-01
EP0201153A2 (en) 1986-11-12
ES8801706A1 (es) 1988-02-16
ES8801707A1 (es) 1988-02-16
AU5330286A (en) 1986-08-14
DK62586A (da) 1986-08-10
ES557478A0 (es) 1988-02-16
ES8800354A1 (es) 1987-10-16
ES551770A0 (es) 1987-07-01
PT81999B (en) 1987-12-22
DK62586D0 (da) 1986-02-07
GR860364B (en) 1986-06-10
ES557477A0 (es) 1987-10-16
EP0201153A3 (en) 1987-10-07
ES557479A0 (es) 1988-02-16

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