ES8706724A1 - Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado. - Google Patents
Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.Info
- Publication number
- ES8706724A1 ES8706724A1 ES551770A ES551770A ES8706724A1 ES 8706724 A1 ES8706724 A1 ES 8706724A1 ES 551770 A ES551770 A ES 551770A ES 551770 A ES551770 A ES 551770A ES 8706724 A1 ES8706724 A1 ES 8706724A1
- Authority
- ES
- Spain
- Prior art keywords
- preparation
- modified enzyme
- lysine
- amino acid
- place
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
METODO DE PREPARAR ACTIVADOR DE PLASMINOGENO DE TIPO TEJIDO MODIFICADO. CONSISTE EN: A) EXPRESAR ADN CODIFICANTE DEL ACTIVADOR DE PLASMINOGENO DEL TIPO TEJIDO MODIFICADO EN UNA CELULA HUESPED RECOMBINANTE BAJO CONDICIONES QUE PERMITAN LA EXPRESION DEL POLIMERO DE ADN, TALES COMO EN PRESENCIA DE UN MEDIO NUTRIENTE, A TEMPERATURA COMPRENDIDA ENTRE LA AMBIENTE Y 45GC, HASTA QUE POR LO MENOS UNA CANTIDAD DETECTABLE DEL PRODUCTO DE EXPRESION PUEDA SER RECUPERADA DLE MEDIO; Y B) RECUPERAR EL PORDUCTO ACTIVADO DE PLASMINOGENO DE TIPO TEJIDO MODIFICADO MEDIANTE AISLAMIENTO DEL PRODUCTO DEL MEDIO NUTRIENTE, SEGUIDO DE LAS DE LA CELULA EFECTUADA FISICA, QUIMICA O ENZIMATICAMENTE. TIENE APLICACIONES FARMACEUTICAS EN ENFERMEDADES TROMBOTICAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB858503358A GB8503358D0 (en) | 1985-02-09 | 1985-02-09 | Compounds |
GB868600979A GB8600979D0 (en) | 1986-01-16 | 1986-01-16 | Compounds |
Publications (2)
Publication Number | Publication Date |
---|---|
ES551770A0 ES551770A0 (es) | 1987-07-01 |
ES8706724A1 true ES8706724A1 (es) | 1987-07-01 |
Family
ID=26288793
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES551770A Expired ES8706724A1 (es) | 1985-02-09 | 1986-02-07 | Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado. |
ES557477A Expired ES8800354A1 (es) | 1985-02-09 | 1987-03-31 | Un procedimiento para preparar un polimero de adn |
ES557478A Expired ES8801706A1 (es) | 1985-02-09 | 1987-03-31 | Un procedimiento para preparar un vector de expresion replicable |
ES557479A Expired ES8801707A1 (es) | 1985-02-09 | 1987-03-31 | Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES557477A Expired ES8800354A1 (es) | 1985-02-09 | 1987-03-31 | Un procedimiento para preparar un polimero de adn |
ES557478A Expired ES8801706A1 (es) | 1985-02-09 | 1987-03-31 | Un procedimiento para preparar un vector de expresion replicable |
ES557479A Expired ES8801707A1 (es) | 1985-02-09 | 1987-03-31 | Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0201153A3 (es) |
AU (1) | AU5330286A (es) |
DK (1) | DK62586A (es) |
ES (4) | ES8706724A1 (es) |
GR (1) | GR860364B (es) |
PT (1) | PT81999B (es) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661469A (en) * | 1984-08-08 | 1987-04-28 | Survival Technology, Inc. | t-PA composition capable of being absorbed into the blood stream and method of administration |
CA1264030A (en) * | 1984-08-08 | 1989-12-27 | Stanley Jay Sarnoff | Protein absorption enhancing agents |
US5736134A (en) * | 1985-04-22 | 1998-04-07 | Genentech, In.C | Tissue plasminogen activator variants |
US5714372A (en) * | 1985-04-22 | 1998-02-03 | Genentech, Inc. | Tissue plasminogen activator variants |
DE3682104D1 (de) * | 1985-04-22 | 1991-11-28 | Genentech Inc | Plasminogenaktivator-mutante des menschlichen gewebes, verfahren und zwischenprodukte dafuer und diese mutante verwendende zusammensetzungen. |
US5756093A (en) * | 1985-04-22 | 1998-05-26 | Genentech, Inc. | Tissue plasminogen activator variants |
US4929444A (en) * | 1985-05-28 | 1990-05-29 | Burroughs Wellcome Co. | Low pH pharmaceutical formulation containing t-PA |
ATE74379T1 (de) * | 1985-12-23 | 1992-04-15 | Chiron Corp | Peptidplasminogenaktivatoren. |
DK43387A (da) * | 1986-01-29 | 1987-07-30 | Beecham Group Plc | Fibrinolytisk enzym |
PT84589B (en) * | 1986-04-02 | 1989-05-09 | Beecham Group Plc | Process for preparing a fibrinolytic enzyme |
PT84588B (en) * | 1986-04-02 | 1989-05-09 | Beecham Group Plc | Process for preparing a fibrinolytic enzyme |
GB8619098D0 (en) * | 1986-08-05 | 1986-09-17 | Wellcome Found | Combination |
DK323587D0 (da) * | 1987-06-25 | 1987-06-25 | Novo Industri As | Protein |
WO1988005081A2 (en) * | 1986-12-30 | 1988-07-14 | Cetus Corporation | Novel plasminogen activator |
US5302390A (en) * | 1987-07-01 | 1994-04-12 | Beecham Group Plc | Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment |
IL87276A (en) * | 1987-08-03 | 1995-07-31 | Fujisawa Pharmaceutical Co | Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it |
US5714145A (en) * | 1988-09-02 | 1998-02-03 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
US5108901A (en) * | 1988-09-02 | 1992-04-28 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
US5262170A (en) * | 1988-09-02 | 1993-11-16 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells |
US5258180A (en) * | 1988-09-02 | 1993-11-02 | Genetech, Inc. | Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment |
US5580560A (en) * | 1989-11-13 | 1996-12-03 | Novo Nordisk A/S | Modified factor VII/VIIa |
US5087572A (en) * | 1989-12-01 | 1992-02-11 | Genentech, Inc. | Dna encoding human plasminogen modified at the cleavage site |
US5190756A (en) * | 1989-12-01 | 1993-03-02 | Genentech, Inc. | Methods and materials for expression of human plasminogen variant |
JP3559559B2 (ja) * | 1992-06-03 | 2004-09-02 | ジェネンテク,インコーポレイテッド | 向上した治療特性を有する組織プラスミノーゲン活性化因子グリコシル化変異体 |
ES2066697B1 (es) * | 1992-12-29 | 1995-08-16 | Azkoyen Ind Sa | Perfeccionamientos introducidos en columnas expendedoras automaticas de productos. |
WO1997033984A1 (en) * | 1996-03-12 | 1997-09-18 | Novo Nordisk A/S | Novel achromobacter lyticus protease variants |
ES2301178T3 (es) | 1996-04-05 | 2008-06-16 | Novartis Vaccines & Diagnostic | Vectores basados en alfavirus recombinantes con inhibicion reducida de la sintesis macromolecular celular. |
US6706504B1 (en) | 1996-11-12 | 2004-03-16 | The Scripps Research Institute | Tissue type plasminogen activator (t-PA) variants: compositions and methods of use |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GR79202B (es) * | 1982-05-05 | 1984-10-22 | Genentech Inc | |
US4753879A (en) * | 1984-08-27 | 1988-06-28 | Biogen N.V. | Modified tissue plasminogen activators |
DE3682104D1 (de) * | 1985-04-22 | 1991-11-28 | Genentech Inc | Plasminogenaktivator-mutante des menschlichen gewebes, verfahren und zwischenprodukte dafuer und diese mutante verwendende zusammensetzungen. |
-
1986
- 1986-02-06 EP EP86300788A patent/EP0201153A3/en not_active Ceased
- 1986-02-07 AU AU53302/86A patent/AU5330286A/en not_active Abandoned
- 1986-02-07 GR GR860364A patent/GR860364B/el unknown
- 1986-02-07 ES ES551770A patent/ES8706724A1/es not_active Expired
- 1986-02-07 DK DK62586A patent/DK62586A/da not_active Application Discontinuation
- 1986-02-07 PT PT8199986A patent/PT81999B/pt unknown
-
1987
- 1987-03-31 ES ES557477A patent/ES8800354A1/es not_active Expired
- 1987-03-31 ES ES557478A patent/ES8801706A1/es not_active Expired
- 1987-03-31 ES ES557479A patent/ES8801707A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES551770A0 (es) | 1987-07-01 |
ES557477A0 (es) | 1987-10-16 |
ES557479A0 (es) | 1988-02-16 |
ES557478A0 (es) | 1988-02-16 |
DK62586D0 (da) | 1986-02-07 |
PT81999A (en) | 1986-03-01 |
AU5330286A (en) | 1986-08-14 |
EP0201153A2 (en) | 1986-11-12 |
DK62586A (da) | 1986-08-10 |
EP0201153A3 (en) | 1987-10-07 |
ES8801706A1 (es) | 1988-02-16 |
ES8801707A1 (es) | 1988-02-16 |
PT81999B (en) | 1987-12-22 |
ES8800354A1 (es) | 1987-10-16 |
GR860364B (en) | 1986-06-10 |
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