ES8704202A1 - Un procedimiento para preparar el plasmido pksr101 - Google Patents

Un procedimiento para preparar el plasmido pksr101

Info

Publication number
ES8704202A1
ES8704202A1 ES550865A ES550865A ES8704202A1 ES 8704202 A1 ES8704202 A1 ES 8704202A1 ES 550865 A ES550865 A ES 550865A ES 550865 A ES550865 A ES 550865A ES 8704202 A1 ES8704202 A1 ES 8704202A1
Authority
ES
Spain
Prior art keywords
subtilis
expression plasmids
plasmids useful
prochymosin
vector system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES550865A
Other languages
English (en)
Other versions
ES550865A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES8704202A1 publication Critical patent/ES8704202A1/es
Publication of ES550865A0 publication Critical patent/ES550865A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Peptides Or Proteins (AREA)

Abstract

METODO DE PRODUCCION DEL PLASMIDO PKSR101. CONSISTE EN DIGERIR DOBLEMENTE EL PLASMIDO PKS3 POR BAM HI Y SAL I PARA AISLAR EL FRAGMENTO (E) DE DNA, QUE CONTIENE LA PARTE C3M DE 4,3 KB, E INCLUYE EL PROMOTOR ERM Y UNA PARTE DEL GEN DE LA PROTEINA 29 K; DIGERIR COMPLETAMENTE EL PLASMIDO PCR702 CON BAM HI Y ECO RI PARA AISLAR EL FRAGMENTO (F) DE DNA FORMADO POR 465 BP; DIGERIR EL PLASMIDO PCR702 DOBLEMENTE CON ECO RI Y SAL I PARA AISLAR EL FRAGMENTO (G) DE DNA; Y LIGAR LOS FRAGMENTOS (E), (F) Y (G) DE DNA POR MEDIO DE LIGASA T4 PARA OBTENER EL PLASMIDO DESEADO. ESTE PLASMIDO TIENE APLICACIONES PARA, TRANSFORMADO EN B. SUBTILIS, PRODUCIR PROQUIMOSINA DE TERNERA.-
ES550865A 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pksr101 Expired ES8704202A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59044900A JPS60188093A (ja) 1984-03-09 1984-03-09 枯草菌に於ける形質発現プラスミド

Publications (2)

Publication Number Publication Date
ES8704202A1 true ES8704202A1 (es) 1987-03-16
ES550865A0 ES550865A0 (es) 1987-03-16

Family

ID=12704349

Family Applications (4)

Application Number Title Priority Date Filing Date
ES541120A Expired ES8606495A1 (es) 1984-03-09 1985-03-08 Un procedimiento para producir proquimosina
ES550863A Expired ES8704204A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pks3
ES550864A Expired ES8704201A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr702
ES550865A Expired ES8704202A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pksr101

Family Applications Before (3)

Application Number Title Priority Date Filing Date
ES541120A Expired ES8606495A1 (es) 1984-03-09 1985-03-08 Un procedimiento para producir proquimosina
ES550863A Expired ES8704204A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pks3
ES550864A Expired ES8704201A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr702

Country Status (9)

Country Link
EP (1) EP0154351B1 (es)
JP (1) JPS60188093A (es)
AT (1) ATE66250T1 (es)
AU (1) AU593524B2 (es)
CA (1) CA1339364C (es)
DE (1) DE3583752D1 (es)
DK (1) DK107785A (es)
ES (4) ES8606495A1 (es)
IE (1) IE57952B1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI841288A (fi) * 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant-dna-kod foer uttryckning av polypeptid.
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
TW201118170A (en) * 2009-11-20 2011-06-01 Ind Tech Res Inst Expression vector for expressing recombinant protein in cyanobacterium

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
BR8202422A (pt) * 1981-04-29 1983-04-12 Biogen Nv Vetor de clonacao de bacillus processo para sua construcao molecula de dna recombinante processo para sua construcao e processo para a producao de um polipeptidio
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production
JPS59166086A (ja) * 1983-03-09 1984-09-19 Teruhiko Beppu 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
NZ208806A (en) * 1983-07-06 1988-07-28 Gist Brocades Nv Genetic engineering of industrial microorganism species: readily-transformable host used as intermediate in transfer of dna to the industrial species; plasmids

Also Published As

Publication number Publication date
ES541120A0 (es) 1986-04-16
EP0154351A3 (en) 1987-08-26
DK107785D0 (da) 1985-03-08
EP0154351A2 (en) 1985-09-11
CA1339364C (en) 1997-08-26
ES550863A0 (es) 1987-03-16
ES8704204A1 (es) 1987-03-16
DE3583752D1 (de) 1991-09-19
ATE66250T1 (de) 1991-08-15
DK107785A (da) 1985-09-10
ES550865A0 (es) 1987-03-16
AU593524B2 (en) 1990-02-15
EP0154351B1 (en) 1991-08-14
IE850605L (en) 1985-09-09
IE57952B1 (en) 1993-05-19
ES8704201A1 (es) 1987-03-16
JPS60188093A (ja) 1985-09-25
AU3963885A (en) 1985-09-12
ES8606495A1 (es) 1986-04-16
ES550864A0 (es) 1987-03-16

Similar Documents

Publication Publication Date Title
GB8610600D0 (en) Transformation of trichoderma
DE3667113D1 (en) Fuel pressure regulator
GR3006248T3 (es)
GB8712936D0 (en) Induction of gene expression in yeast
PT81584B (en) Process for the isolation and detection of discrete mammalian genes associated with tumor metastases
ES8704204A1 (es) Un procedimiento para preparar el plasmido pks3
DE3381246D1 (en) Alpha-interferon gx-1.
IL75440A0 (en) Nif promoter of rhizobium japonicum bacterial strains and plasmids containing the same and methods for the use thereof
EP0182562A3 (en) Process for a thermo-inducible gene expression in gram positive bacteria, bacillus subtilis strains containing plasmids which induce such an expression and portable vector systems
AU5051290A (en) Process for use with pressurized reactors
HUT40162A (en) Recombinant dna expression vectors and process for gene expression
ES8703932A1 (es) Un procedimiento para preparar una molecula de adn recombinante.
EP0154186A3 (en) Isolation of a novel interferon gene and expression thereof
ES8706641A1 (es) Procedimiento para preparar derivados de pirazol y bencimidazol.
ZA868544B (en) Streptomycin sensitive gene having a lot of cloning sites,plasmid vector containing the same and microorganism containing the vector
IL74536A (en) Expression plasmid for prochymosin in bacillus subtilis and process for its preparation
AU6363786A (en) Battery for sonabuoy having sleeve for disposing of gas bubbles
SU1259554A1 (ru) Шарнир универсального шпинделя
IE852696L (en) Gene regulator
ZA867506B (en) Method of enhancing t-pa and scu-pa production
AU8255782A (en) Production and use of informatory rna
IE853034L (en) Multi-copy yeast vectors
LTIP1527A (en) Cloning and expression of microbial phytase
SU1266402A1 (ru) Способ анизотропного травления мезоструктур

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20040601