ES8606495A1 - Un procedimiento para producir proquimosina - Google Patents

Un procedimiento para producir proquimosina

Info

Publication number
ES8606495A1
ES8606495A1 ES541120A ES541120A ES8606495A1 ES 8606495 A1 ES8606495 A1 ES 8606495A1 ES 541120 A ES541120 A ES 541120A ES 541120 A ES541120 A ES 541120A ES 8606495 A1 ES8606495 A1 ES 8606495A1
Authority
ES
Spain
Prior art keywords
subtilis
expression plasmids
plasmids useful
prochymosin
vector system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES541120A
Other languages
English (en)
Other versions
ES541120A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES8606495A1 publication Critical patent/ES8606495A1/es
Publication of ES541120A0 publication Critical patent/ES541120A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE PROQUIMOSINA. CONSISTE EN INSERTAR UN GEN DE PROQUIMOSINA EN UN SISTEMA VECTOR DE B. SUBTILIS, QUE CONTIENE UN PROMOTOR, CON EL SISTEMA DE LECTURA APROPIADO, PARA FORMAR PLASMIDOS DE EXPRESION; TRANSFORMAR UN ORGANICOS HUESPED B. SUBTILIS CON DICHO PLASMIDO DE EXPRESION PARA OBTENER TRANSFORMANTES; SELECCIONAR EL TRANSFORMANTE RESISTENTE AL FARMACO; CULTIVAR DICHO TRANSFORMANTE EN UN MEDIO NUTRIENTE ADECUADO; Y, FINALMENTE, AISLAR PROQUIMOSINA A PARTIR DE LOS CULTIVOS OBTENIDOS. TIENE APLICACIONES PARA OBTENER, MEDIANTE LA TECNOLOGIA DEL DNA RECOMBINANTE, UN PROMOTOR RESISTENTE A LA ERITROMICINA.
ES541120A 1984-03-09 1985-03-08 Un procedimiento para producir proquimosina Expired ES8606495A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59044900A JPS60188093A (ja) 1984-03-09 1984-03-09 枯草菌に於ける形質発現プラスミド

Publications (2)

Publication Number Publication Date
ES8606495A1 true ES8606495A1 (es) 1986-04-16
ES541120A0 ES541120A0 (es) 1986-04-16

Family

ID=12704349

Family Applications (4)

Application Number Title Priority Date Filing Date
ES541120A Expired ES8606495A1 (es) 1984-03-09 1985-03-08 Un procedimiento para producir proquimosina
ES550863A Expired ES8704204A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pks3
ES550865A Expired ES8704202A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pksr101
ES550864A Expired ES8704201A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr702

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES550863A Expired ES8704204A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pks3
ES550865A Expired ES8704202A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pksr101
ES550864A Expired ES8704201A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr702

Country Status (9)

Country Link
EP (1) EP0154351B1 (es)
JP (1) JPS60188093A (es)
AT (1) ATE66250T1 (es)
AU (1) AU593524B2 (es)
CA (1) CA1339364C (es)
DE (1) DE3583752D1 (es)
DK (1) DK107785A (es)
ES (4) ES8606495A1 (es)
IE (1) IE57952B1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123928A3 (en) * 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
TW201118170A (en) * 2009-11-20 2011-06-01 Ind Tech Res Inst Expression vector for expressing recombinant protein in cyanobacterium

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
AU8300282A (en) * 1981-04-29 1982-11-04 Biogen N.V. Bacillus cloning vectors and method of expressing foreign dna
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production
JPS59166086A (ja) * 1983-03-09 1984-09-19 Teruhiko Beppu 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
JP2669457B2 (ja) * 1983-07-06 1997-10-27 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 工業微生物種中の分子クローニングおよび発現

Also Published As

Publication number Publication date
EP0154351B1 (en) 1991-08-14
EP0154351A3 (en) 1987-08-26
DE3583752D1 (de) 1991-09-19
ES8704204A1 (es) 1987-03-16
ES550863A0 (es) 1987-03-16
ES550865A0 (es) 1987-03-16
DK107785A (da) 1985-09-10
AU3963885A (en) 1985-09-12
ATE66250T1 (de) 1991-08-15
ES8704201A1 (es) 1987-03-16
CA1339364C (en) 1997-08-26
JPS60188093A (ja) 1985-09-25
IE850605L (en) 1985-09-09
EP0154351A2 (en) 1985-09-11
ES541120A0 (es) 1986-04-16
DK107785D0 (da) 1985-03-08
ES550864A0 (es) 1987-03-16
IE57952B1 (en) 1993-05-19
AU593524B2 (en) 1990-02-15
ES8704202A1 (es) 1987-03-16

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20020401