ES8703519A1 - Procedimiento para la formacion de hibridos celulares - Google Patents
Procedimiento para la formacion de hibridos celularesInfo
- Publication number
- ES8703519A1 ES8703519A1 ES555123A ES555123A ES8703519A1 ES 8703519 A1 ES8703519 A1 ES 8703519A1 ES 555123 A ES555123 A ES 555123A ES 555123 A ES555123 A ES 555123A ES 8703519 A1 ES8703519 A1 ES 8703519A1
- Authority
- ES
- Spain
- Prior art keywords
- cells
- hybrid cells
- hepatitis
- hybrid
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000282693 Cercopithecidae Species 0.000 title abstract 3
- 210000004754 hybrid cell Anatomy 0.000 title abstract 3
- 241000700721 Hepatitis B virus Species 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 title abstract 2
- 210000003494 hepatocyte Anatomy 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000427 antigen Substances 0.000 title 2
- 102000036639 antigens Human genes 0.000 title 2
- 108091007433 antigens Proteins 0.000 title 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000003501 co-culture Methods 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 241000282577 Pan troglodytes Species 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Silicates, Zeolites, And Molecular Sieves (AREA)
Abstract
PROCEDIMIENTO PARA FORMAR HIBRIDOS CELULARES. COMPRENDE: A) CLONAR A LAS CELULAS ESTABLECIDAS DE CHIMPANCE Y A LOS HEPATOCITOS DE CHIMPANCE CON UNA SECUANDIA DE ADN CLONADA; B) COCULTIVAR A LAS CELULAS EUROCARIOTAS ESTABLECIDAS CLONADAS Y A LOS HEPATOCITOS CLONADOS, EN UN MEDIO QUE NO PERMITE EL DESARROLLO DE LAS CELULAS ESTABLECIDAS, CUANDO NO ESTAN COMPLEMENTADOS POR UNA SECUENCIA GENETICA APROPIADA APORTADA POR LOS HEPATOCITOS, PARA HIBRIDARLOS; Y C) SELECCIONAR A LOS HIBRIDOS CELULARES QUE SEAN PRODUCTORES DE POLIPEPTIDOS CODIFICADOS DE ADN CLONADO. LAS CELULAS ESTABLECIDAS DE MONO, SON CELULAS DE LINERO VERO COMO HGPRT. EL MEDIO DE CULTIVO CONTIENE UN ANTIBIOTICO AMINOGLICOSIDO COMO G418 O AMINOGLICOSIDO 3K-FOSFOTRANSFERASA Y LA SECUENCIA DE ADN CLONADA TIENE UNA PARTE DE GEN S DE VIRUS HEPATITIS VIRAL B Y UNA REGION PRE-S DEL GENOMA DEL VIRUS DE LA HEPATITIS VIRAL B.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR858506706A FR2581393B2 (fr) | 1985-05-02 | 1985-05-02 | Cellules hybrides productrices d'un antigene caracteristique du virus de l'hepatite b obtenu a partir d'hepatocytes et de cellules etablies de singe, procede pour l'obtention de ces cellules hybrides et application de celles-ci a la production de l'antigene susdit |
Publications (2)
Publication Number | Publication Date |
---|---|
ES555123A0 ES555123A0 (es) | 1987-03-01 |
ES8703519A1 true ES8703519A1 (es) | 1987-03-01 |
Family
ID=9318906
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES555123A Expired ES8703519A1 (es) | 1985-05-02 | 1986-05-02 | Procedimiento para la formacion de hibridos celulares |
Country Status (12)
Country | Link |
---|---|
US (1) | US5112748A (es) |
EP (1) | EP0200653B1 (es) |
JP (1) | JPS6229971A (es) |
AT (1) | ATE51026T1 (es) |
CA (1) | CA1277934C (es) |
DE (1) | DE3669529D1 (es) |
DK (1) | DK204586A (es) |
ES (1) | ES8703519A1 (es) |
FR (1) | FR2581393B2 (es) |
GR (1) | GR861142B (es) |
PT (1) | PT82505B (es) |
WO (1) | WO1993013203A1 (es) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7766329B1 (en) | 1992-10-02 | 2010-08-03 | Sierra Design Group | Wheel indicator and ticket dispenser apparatus |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2757169A1 (de) * | 1977-12-22 | 1979-07-05 | Hoechst Ag | Verfahren zur gewinnung insulin produzierender tierischer zellen |
FR2458584A1 (fr) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | Vecteurs pour le transfert et l'expression de materiel genetique dans une cellule eucaryote et procede pour la production d'une proteine determinee dans des cellules eucaryotes |
FR2480780B1 (fr) * | 1980-04-22 | 1985-12-06 | Pasteur Institut | Procede de transformation de cellules, notamment eucaryotes, par un adn circulaire tel que celui du virus de l'hepatite b et preparations contenant les produits d'expression desdits adn |
JPS6045848B2 (ja) * | 1980-07-31 | 1985-10-12 | 株式会社林原生物化学研究所 | ヒト成長ホルモンの製造方法 |
FR2500847B1 (fr) * | 1981-03-02 | 1985-09-13 | Pasteur Institut | Marqueurs genetiques selectifs pour cellules eucaryotes, procede de mise en oeuvre de tels marqueurs et application des cellules contenant un tel marqueur a la fabrication de proteines determinees apres leur transformation par un adn correspondant |
EP0062574A3 (en) * | 1981-03-31 | 1982-12-29 | The Regents Of The University Of California | Virus protein synthesis |
DE3245665A1 (de) * | 1982-05-04 | 1983-11-10 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur herstellung permanenter tierischer und humaner zellinien und deren verwendung |
JPS58201988A (ja) * | 1982-05-04 | 1983-11-25 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | 永久培養可能な動物及びヒトの細胞系を得る方法 |
US4608339A (en) * | 1983-10-25 | 1986-08-26 | Yoakum George H | Protoplast fusion method for high-frequency DNA transfection in human cells |
FR2555607B2 (fr) * | 1983-11-30 | 1986-10-03 | Pasteur Institut | Culture de cellules eucaryotes productrices d'une proteine vaccinante, notamment d'antigene hbs |
FR2565995B1 (fr) * | 1984-03-07 | 1987-10-23 | Centre Nat Rech Scient | Cellules hybrides productrices d'un polypeptide determine et obtenu a partir de cellules primaires naturellement capables d'exprimer ce polypeptide ou un polypeptide equivalent, procede pour l'obtention de ces cellules hybrides et application de celles-ci a la production dudit polypeptide |
-
1985
- 1985-05-02 FR FR858506706A patent/FR2581393B2/fr not_active Expired
-
1986
- 1986-04-29 DE DE8686400945T patent/DE3669529D1/de not_active Expired - Lifetime
- 1986-04-29 US US07/031,465 patent/US5112748A/en not_active Expired - Fee Related
- 1986-04-29 WO PCT/FR1986/000147 patent/WO1993013203A1/fr unknown
- 1986-04-29 AT AT86400945T patent/ATE51026T1/de not_active IP Right Cessation
- 1986-04-29 EP EP86400945A patent/EP0200653B1/fr not_active Expired - Lifetime
- 1986-04-29 GR GR861142A patent/GR861142B/el unknown
- 1986-05-02 PT PT82505A patent/PT82505B/pt not_active IP Right Cessation
- 1986-05-02 CA CA000508230A patent/CA1277934C/fr not_active Expired - Fee Related
- 1986-05-02 JP JP61102930A patent/JPS6229971A/ja active Pending
- 1986-05-02 ES ES555123A patent/ES8703519A1/es not_active Expired
- 1986-05-02 DK DK204586A patent/DK204586A/da not_active Application Discontinuation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7766329B1 (en) | 1992-10-02 | 2010-08-03 | Sierra Design Group | Wheel indicator and ticket dispenser apparatus |
Also Published As
Publication number | Publication date |
---|---|
ES555123A0 (es) | 1987-03-01 |
GR861142B (en) | 1986-08-11 |
PT82505A (fr) | 1986-06-01 |
FR2581393A2 (fr) | 1986-11-07 |
US5112748A (en) | 1992-05-12 |
JPS6229971A (ja) | 1987-02-07 |
PT82505B (pt) | 1989-12-29 |
EP0200653B1 (fr) | 1990-03-14 |
EP0200653A1 (fr) | 1986-11-05 |
FR2581393B2 (fr) | 1989-07-21 |
CA1277934C (fr) | 1990-12-18 |
DK204586D0 (da) | 1986-05-02 |
DK204586A (da) | 1986-11-03 |
DE3669529D1 (de) | 1990-04-19 |
ATE51026T1 (de) | 1990-03-15 |
WO1993013203A1 (fr) | 1993-07-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970303 |