CA2154854A1 - Immortalized lymphocytes for production of viral-free proteins - Google Patents
Immortalized lymphocytes for production of viral-free proteinsInfo
- Publication number
- CA2154854A1 CA2154854A1 CA002154854A CA2154854A CA2154854A1 CA 2154854 A1 CA2154854 A1 CA 2154854A1 CA 002154854 A CA002154854 A CA 002154854A CA 2154854 A CA2154854 A CA 2154854A CA 2154854 A1 CA2154854 A1 CA 2154854A1
- Authority
- CA
- Canada
- Prior art keywords
- immortalized
- lymphocytes
- proteins
- ebv
- human lymphocytes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
This invention is directed to construction of recombinant plasmids that contain the Epstein-Barr virus ("EBV") genetic information involved in the immortalization of human lymphocytes, but that lack one or more genes implicated in EBV lytic replication. The recombinant plasmids have the advantage of being able to immortalize human lymphocytes, while being incapable of generating infectious EBV particles. The invention also provides human lymphocytes that have been successfully infected and immortalized by the recombinant plasmids. These human lymphocyte clones can be used as cellular factories to produce desired proteins and as delivery vehicles for gene therapy. Additionally, the invention establishes a method by which desired proteins can be produced in immortalized cellular factories, without generating infectious EBV particles. These proteins can be the normal cellular products of immortalized human lymphocytes, or they can be proteins encoded by foreign genes, which have been cloned into the lymphocytes.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4427117A DE4427117C1 (en) | 1994-07-30 | 1994-07-30 | Plasmids contg. specified Epstein-Barr virus sequences |
DEP4427117.4 | 1994-07-30 | ||
US08/504,494 | 1995-07-20 | ||
US08/504,494 US5716845A (en) | 1995-07-20 | 1995-07-20 | Immortalized lymphocytes for production of viral-free proteins |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2154854A1 true CA2154854A1 (en) | 1996-01-31 |
CA2154854C CA2154854C (en) | 2006-03-14 |
Family
ID=25938843
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002154854A Expired - Fee Related CA2154854C (en) | 1994-07-30 | 1995-07-27 | Immortalized lymphocytes for production of viral-free proteins |
Country Status (2)
Country | Link |
---|---|
JP (1) | JPH08168382A (en) |
CA (1) | CA2154854C (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR112020012361A2 (en) * | 2017-12-20 | 2020-11-24 | Glaxosmithkline Biologicals S.A. | epstein-barr virus antigen constructs |
-
1995
- 1995-07-27 CA CA002154854A patent/CA2154854C/en not_active Expired - Fee Related
- 1995-07-28 JP JP7224475A patent/JPH08168382A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA2154854C (en) | 2006-03-14 |
JPH08168382A (en) | 1996-07-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HK1018078A1 (en) | Recombinant sendai virus | |
HK1018287A1 (en) | (-)-strand rna virus vector having autonomously replicating activity | |
AU6131086A (en) | Epithelial cells expressing foreign genetic material | |
EP0762894A4 (en) | Method of inducing an immune response with a live venezuelan equine encephalitis virus expressing a heterologous immunogen | |
HU9603155D0 (en) | Papillomavirus vaccines | |
EP0120551A3 (en) | Yeast expression systems with vectors having gapdh or pyk promoters, and synthesis of foreign proteins | |
EP1018555A3 (en) | Chimeric papilomavirus-like particles | |
AU560340B2 (en) | Rennin from recombinant dna | |
AU2347997A (en) | Synthesis and purification of hepatitis c virus-like particles | |
EP1394259A3 (en) | Recombinant infectious non-segmented negative strand RNA virus | |
IE872455L (en) | Improved recombinant expression | |
EP0732340A3 (en) | Expression of porcine reproductive respiratory syndrome virus polypeptides in the same cell | |
EP0540645A4 (en) | Methods of use of bovine respiratory syncytial virus recombinant dna, proteins vaccines, antibodies, and transformed cells | |
US5627155A (en) | BCRF1 proteins as inhibitors of interferon-γ | |
HUP9801334A2 (en) | Dna encoding human papilloma virus type 18 | |
US4808523A (en) | Constitutive production of human IFN-β1 by mammalian cells transformed by the IFN-β1 gene fused to an SV40 early promoter | |
ES8600939A1 (en) | Recombinant DNA molecules encoding for hepatitis B surface antigen, their preparation and derived vaccines. | |
CA2154854A1 (en) | Immortalized lymphocytes for production of viral-free proteins | |
US5716845A (en) | Immortalized lymphocytes for production of viral-free proteins | |
JO1885B1 (en) | Process for purifying hepatitis bviral surface antigen comprising pres2 peptide | |
EP0592685A4 (en) | Plant resistant to two or more viruses and preparation thereof. | |
WO1996009389A3 (en) | Recombinant macrophage migration inhibitory factor; expression in escherichia coli and purification of recombinant protein | |
EP0694613A1 (en) | Plasmid and transfected cello therewith | |
ES8703519A1 (en) | Hybrid cells producing a hepatitis B virus antigen obtained from hepatocytes and established monkey cells, process for the obtention of these hybrid cells and use thereof in the production of this antigen. | |
TW251319B (en) | A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |