CA2154854A1 - Immortalized lymphocytes for production of viral-free proteins - Google Patents

Immortalized lymphocytes for production of viral-free proteins

Info

Publication number
CA2154854A1
CA2154854A1 CA002154854A CA2154854A CA2154854A1 CA 2154854 A1 CA2154854 A1 CA 2154854A1 CA 002154854 A CA002154854 A CA 002154854A CA 2154854 A CA2154854 A CA 2154854A CA 2154854 A1 CA2154854 A1 CA 2154854A1
Authority
CA
Canada
Prior art keywords
immortalized
lymphocytes
proteins
ebv
human lymphocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002154854A
Other languages
French (fr)
Other versions
CA2154854C (en
Inventor
William M. Sugden
Wolfgang F. Hammerschmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4427117A external-priority patent/DE4427117C1/en
Priority claimed from US08/504,494 external-priority patent/US5716845A/en
Application filed by Individual filed Critical Individual
Publication of CA2154854A1 publication Critical patent/CA2154854A1/en
Application granted granted Critical
Publication of CA2154854C publication Critical patent/CA2154854C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

This invention is directed to construction of recombinant plasmids that contain the Epstein-Barr virus ("EBV") genetic information involved in the immortalization of human lymphocytes, but that lack one or more genes implicated in EBV lytic replication. The recombinant plasmids have the advantage of being able to immortalize human lymphocytes, while being incapable of generating infectious EBV particles. The invention also provides human lymphocytes that have been successfully infected and immortalized by the recombinant plasmids. These human lymphocyte clones can be used as cellular factories to produce desired proteins and as delivery vehicles for gene therapy. Additionally, the invention establishes a method by which desired proteins can be produced in immortalized cellular factories, without generating infectious EBV particles. These proteins can be the normal cellular products of immortalized human lymphocytes, or they can be proteins encoded by foreign genes, which have been cloned into the lymphocytes.
CA002154854A 1994-07-30 1995-07-27 Immortalized lymphocytes for production of viral-free proteins Expired - Fee Related CA2154854C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE4427117A DE4427117C1 (en) 1994-07-30 1994-07-30 Plasmids contg. specified Epstein-Barr virus sequences
DEP4427117.4 1994-07-30
US08/504,494 1995-07-20
US08/504,494 US5716845A (en) 1995-07-20 1995-07-20 Immortalized lymphocytes for production of viral-free proteins

Publications (2)

Publication Number Publication Date
CA2154854A1 true CA2154854A1 (en) 1996-01-31
CA2154854C CA2154854C (en) 2006-03-14

Family

ID=25938843

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002154854A Expired - Fee Related CA2154854C (en) 1994-07-30 1995-07-27 Immortalized lymphocytes for production of viral-free proteins

Country Status (2)

Country Link
JP (1) JPH08168382A (en)
CA (1) CA2154854C (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112020012361A2 (en) * 2017-12-20 2020-11-24 Glaxosmithkline Biologicals S.A. epstein-barr virus antigen constructs

Also Published As

Publication number Publication date
CA2154854C (en) 2006-03-14
JPH08168382A (en) 1996-07-02

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