TW251319B - A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen - Google Patents

A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen

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Publication number
TW251319B
TW251319B TW81108310A TW81108310A TW251319B TW 251319 B TW251319 B TW 251319B TW 81108310 A TW81108310 A TW 81108310A TW 81108310 A TW81108310 A TW 81108310A TW 251319 B TW251319 B TW 251319B
Authority
TW
Taiwan
Prior art keywords
antigen
hepatitis
virus
plasmid
granule
Prior art date
Application number
TW81108310A
Other languages
Chinese (zh)
Inventor
Peir-Jer Chen
Ding-Shinn Chen
Original Assignee
Nat Science Committee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Science Committee filed Critical Nat Science Committee
Priority to TW81108310A priority Critical patent/TW251319B/en
Application granted granted Critical
Publication of TW251319B publication Critical patent/TW251319B/en

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Abstract

A preparation process for Hepatitis B surface antigen granule carryingHepatitis delta virus antigen, which comprises using molecular biological methodology of using plasmid pslx exhibiting Hepatitis B surface antigen and plasmids (large D being pSVLAg-L, small D being pSVLAg-S) exhibiting Hepatitis delta virus antigen, transfecting the plasmids into liver cancer clone by calcium phosphate precipitation, purifying the virus granules, in which the Hepatitis B surface antigen granule carrying Hepatitis delta virus is divided into two types: (1) the Hepatitis delta virus antigen therein is only large D antigen and the plasmid of large D antigen and the plasmid exhibiting Hepatitis B virus are cotransfected into the liver cancer clone; (2) the Hepatitis delta virus antigen comprises large D and small D antigens at the same time, the large D antigen plasmid and small D antigen plasmid and the plasmid exhibiting Hepatitis B virus are cotransfected into the liver cancer clone in which the small D antigen can not be enveloped in the virus granule alone and is done by entering the Hepatitis B virus antigen granule together with large D antigen, and the plasmid of large D antigen and small D antigen and the plasmid of Hepatitis B virus jointly form a Hepatitis B and delta viruses antigen composite granule.
TW81108310A 1992-10-19 1992-10-19 A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen TW251319B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW81108310A TW251319B (en) 1992-10-19 1992-10-19 A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW81108310A TW251319B (en) 1992-10-19 1992-10-19 A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen

Publications (1)

Publication Number Publication Date
TW251319B true TW251319B (en) 1995-07-11

Family

ID=51401365

Family Applications (1)

Application Number Title Priority Date Filing Date
TW81108310A TW251319B (en) 1992-10-19 1992-10-19 A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen

Country Status (1)

Country Link
TW (1) TW251319B (en)

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