TW251319B - A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen - Google Patents
A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigenInfo
- Publication number
- TW251319B TW251319B TW81108310A TW81108310A TW251319B TW 251319 B TW251319 B TW 251319B TW 81108310 A TW81108310 A TW 81108310A TW 81108310 A TW81108310 A TW 81108310A TW 251319 B TW251319 B TW 251319B
- Authority
- TW
- Taiwan
- Prior art keywords
- antigen
- hepatitis
- virus
- plasmid
- granule
- Prior art date
Links
Abstract
A preparation process for Hepatitis B surface antigen granule carryingHepatitis delta virus antigen, which comprises using molecular biological methodology of using plasmid pslx exhibiting Hepatitis B surface antigen and plasmids (large D being pSVLAg-L, small D being pSVLAg-S) exhibiting Hepatitis delta virus antigen, transfecting the plasmids into liver cancer clone by calcium phosphate precipitation, purifying the virus granules, in which the Hepatitis B surface antigen granule carrying Hepatitis delta virus is divided into two types: (1) the Hepatitis delta virus antigen therein is only large D antigen and the plasmid of large D antigen and the plasmid exhibiting Hepatitis B virus are cotransfected into the liver cancer clone; (2) the Hepatitis delta virus antigen comprises large D and small D antigens at the same time, the large D antigen plasmid and small D antigen plasmid and the plasmid exhibiting Hepatitis B virus are cotransfected into the liver cancer clone in which the small D antigen can not be enveloped in the virus granule alone and is done by entering the Hepatitis B virus antigen granule together with large D antigen, and the plasmid of large D antigen and small D antigen and the plasmid of Hepatitis B virus jointly form a Hepatitis B and delta viruses antigen composite granule.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW81108310A TW251319B (en) | 1992-10-19 | 1992-10-19 | A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW81108310A TW251319B (en) | 1992-10-19 | 1992-10-19 | A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen |
Publications (1)
Publication Number | Publication Date |
---|---|
TW251319B true TW251319B (en) | 1995-07-11 |
Family
ID=51401365
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW81108310A TW251319B (en) | 1992-10-19 | 1992-10-19 | A preparation process for Hepatitis B surface antigen granule carrying Hepatitis delta virus antigen |
Country Status (1)
Country | Link |
---|---|
TW (1) | TW251319B (en) |
-
1992
- 1992-10-19 TW TW81108310A patent/TW251319B/en not_active IP Right Cessation
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Legal Events
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MK4A | Expiration of patent term of an invention patent |