KR970010136B1 - A process for preparing hepatitis b virus surface antigen pre-s1 peptide in e. coli - Google Patents

A process for preparing hepatitis b virus surface antigen pre-s1 peptide in e. coli Download PDF

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Publication number
KR970010136B1
KR970010136B1 KR94006459A KR19940006459A KR970010136B1 KR 970010136 B1 KR970010136 B1 KR 970010136B1 KR 94006459 A KR94006459 A KR 94006459A KR 19940006459 A KR19940006459 A KR 19940006459A KR 970010136 B1 KR970010136 B1 KR 970010136B1
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KR
South Korea
Prior art keywords
coli
peptide
surface antigen
virus surface
antigen pre
Prior art date
Application number
KR94006459A
Other languages
Korean (ko)
Other versions
KR950026989A (en
Inventor
Hyo-Jung Hong
Sun-Boon Yeun
Hee-Sun Kim
Original Assignee
Korea Green Cross Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Green Cross Co filed Critical Korea Green Cross Co
Priority to KR94006459A priority Critical patent/KR970010136B1/en
Publication of KR950026989A publication Critical patent/KR950026989A/en
Application granted granted Critical
Publication of KR970010136B1 publication Critical patent/KR970010136B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The method for preparing a lot of soluble pre-S1 antigen for B-type hepatitis virus from colitis germs is designed to produce a fused protein containing a pre-S1 antigen fused with a maltose coupling protein.
KR94006459A 1994-03-30 1994-03-30 A process for preparing hepatitis b virus surface antigen pre-s1 peptide in e. coli KR970010136B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR94006459A KR970010136B1 (en) 1994-03-30 1994-03-30 A process for preparing hepatitis b virus surface antigen pre-s1 peptide in e. coli

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR94006459A KR970010136B1 (en) 1994-03-30 1994-03-30 A process for preparing hepatitis b virus surface antigen pre-s1 peptide in e. coli

Publications (2)

Publication Number Publication Date
KR950026989A KR950026989A (en) 1995-10-16
KR970010136B1 true KR970010136B1 (en) 1997-06-21

Family

ID=19379922

Family Applications (1)

Application Number Title Priority Date Filing Date
KR94006459A KR970010136B1 (en) 1994-03-30 1994-03-30 A process for preparing hepatitis b virus surface antigen pre-s1 peptide in e. coli

Country Status (1)

Country Link
KR (1) KR970010136B1 (en)

Also Published As

Publication number Publication date
KR950026989A (en) 1995-10-16

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