ES8606947A1 - Un inmunoensayo marcado con un inhibidor de enzima - Google Patents

Un inmunoensayo marcado con un inhibidor de enzima

Info

Publication number
ES8606947A1
ES8606947A1 ES529266A ES529266A ES8606947A1 ES 8606947 A1 ES8606947 A1 ES 8606947A1 ES 529266 A ES529266 A ES 529266A ES 529266 A ES529266 A ES 529266A ES 8606947 A1 ES8606947 A1 ES 8606947A1
Authority
ES
Spain
Prior art keywords
assay
milk
sample
enzyme
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES529266A
Other languages
English (en)
Other versions
ES529266A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boots Celltech Diagnostics Ltd
Original Assignee
Boots Celltech Diagnostics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB838302622A external-priority patent/GB8302622D0/en
Application filed by Boots Celltech Diagnostics Ltd filed Critical Boots Celltech Diagnostics Ltd
Publication of ES529266A0 publication Critical patent/ES529266A0/es
Publication of ES8606947A1 publication Critical patent/ES8606947A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0033—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005
    • C07J41/005—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005 the 17-beta position being substituted by an uninterrupted chain of only two carbon atoms, e.g. pregnane derivatives
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO DE INMUNOENSAYO MARCADO CON UN INHIBIDOR ENZIMATICO PARA MEDIR LA CONCENTRACION DE UN ANALITO ANTIGENICO EN UNA MUESTRA. COMPRENDE: A) DEJAR TRANSCURRIR UNA REACCION COMPETITIVA ENTRE (A) EL ANALIZTO ANTIGENICO EN LA MUESTRA, (B) UNA MOLECULA CONJUGADA CON UN RESTO QUE TIENE UN DETERMINANTE ANTIGENICO EN COMUN CON EL ANALITO ANTIGENICO Y ESTA UNIDO A UN INHIBIDOR QUE INHIBE LA ACTIVIDAD DE UNA ENZIMA PARA UN SUSTRATO QUE CONSTITUYE UNA PARTE DE LA MUESTRA, Y (C) UN ANTICUERPO, PARA FORMAR UN ENLACE INMUNOQUIMICO CON LA MOLECULA CONJUGADA Y CON EL ANALITO ANTIGENICO; Y B) DETERMINAR LA ACTIVIDAD DE LA ENZIMA SOBRE EL SUSTRATO, EN PRESENCIA DE (A), (B) Y (C). LA MUESTRA CONSTA DE LECHE; LA ENZIMA ES QUIMOSINA; EL ANALITO ANTIGENICO ES UN ESTEROIDE SELECCIONADO ENTRE PROGESTAGENO, UN METABOLITO DE UN PROGESTAGENO Y UN ESTROGENO; EL INHIBIDOR SE SELECCIONA ENTRE P-SER-Z-ALA-Q, Y P-VAL-Z-VAL-Q. SIENDO: Z, ESTATINA; P, A- P HIS-; A, RADICAL DE LEUCINA; Q, -B, O -B-PRO Y B RADICAL DE ISOLEUCINA Y LA MOLECULA CONJUGADA ES HAPTENO MARCADA.
ES529266A 1983-01-31 1984-01-30 Un inmunoensayo marcado con un inhibidor de enzima Expired ES8606947A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB838302622A GB8302622D0 (en) 1983-01-31 1983-01-31 Immunoassay
GB838320164A GB8320164D0 (en) 1983-01-31 1983-07-26 Immunoassay
GB08400921A GB2135773B (en) 1983-01-31 1984-01-13 Enzyme inhibitor labelled immunoassay

Publications (2)

Publication Number Publication Date
ES529266A0 ES529266A0 (es) 1986-05-01
ES8606947A1 true ES8606947A1 (es) 1986-05-01

Family

ID=27261945

Family Applications (1)

Application Number Title Priority Date Filing Date
ES529266A Expired ES8606947A1 (es) 1983-01-31 1984-01-30 Un inmunoensayo marcado con un inhibidor de enzima

Country Status (7)

Country Link
EP (1) EP0117648A3 (es)
BR (1) BR8400388A (es)
DE (1) DE117648T1 (es)
DK (1) DK18084A (es)
ES (1) ES8606947A1 (es)
GB (1) GB2135773B (es)
IE (1) IE55291B1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE850830L (en) * 1985-04-01 1986-10-01 Noctech Ltd Enzyme immunoassay method
EP0265519B1 (en) * 1986-04-30 1995-09-13 IGEN, INC. (a California corporation) Electrochemiluminescent assays
GB8802838D0 (en) * 1988-02-08 1988-03-09 Microtest Research Ltd Marking of chemical products to establish identity & source
US5508387A (en) * 1993-08-04 1996-04-16 Glycomed Incorporated Selectin binding glycopeptides
DE59510092D1 (de) * 1994-07-25 2002-04-11 Roche Diagnostics Gmbh Hapten-markierte peptide
DE4442722A1 (de) * 1994-12-01 1996-06-05 Basf Ag Schlagzäh thermoplastische Formmassen
BE1010184A3 (fr) * 1996-04-30 1998-02-03 Biocode Sa Procede de detection et/ou de quantification d'un haptene dans une phase homogene et dispositif pour sa mise en oeuvre.
EP2113510A1 (en) * 2008-04-30 2009-11-04 Freie Universität Berlin Pepstatin A derivatives

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4134792A (en) * 1976-12-06 1979-01-16 Miles Laboratories, Inc. Specific binding assay with an enzyme modulator as a labeling substance
GB1595101A (en) * 1977-03-15 1981-08-05 Hoffmann La Roche Enzyme modifier immunoassay
IT1114861B (it) * 1977-05-12 1986-01-27 Sclavo Inst Sieroterapeut Metodo per la determinazione del contenuto di costituenti di fluidi biologici e mezzi adatti allo scopo
GB2040043B (en) * 1978-12-29 1983-03-16 Heap R Method for monitoring pregnancy in milk-producing domestic animals
US4273866A (en) * 1979-02-05 1981-06-16 Abbott Laboratories Ligand analog-irreversible enzyme inhibitor conjugates and methods for use
DE3006710A1 (de) * 1980-02-22 1981-08-27 Hans A. Dipl.-Chem. Dr. 8000 München Thoma Enzymmodulator-ligand-konjugat und verfahren unter dessen anwendung
DE3006709A1 (de) * 1980-02-22 1981-08-27 Hans A. Dipl.-Chem. Dr. 8000 München Thoma Homogenes verfahren zur kompetitiven bestimmung von liganden
NO812612L (no) * 1980-08-06 1982-02-08 Ferring Pharma Ltd Enzym-inhibitorer.
CA1258748A (en) * 1981-10-08 1989-08-22 Daniel F. Veber Renin inhibitory peptides
EP0095286B1 (en) * 1982-05-26 1985-12-18 Boots-Celltech Diagnostics Limited Immunoassay

Also Published As

Publication number Publication date
IE840082L (en) 1984-07-31
EP0117648A2 (en) 1984-09-05
ES529266A0 (es) 1986-05-01
IE55291B1 (en) 1990-08-01
GB8400921D0 (en) 1984-02-15
GB2135773A (en) 1984-09-05
BR8400388A (pt) 1984-09-04
GB2135773B (en) 1985-12-04
DE117648T1 (de) 1985-03-28
EP0117648A3 (en) 1988-02-03
DK18084D0 (da) 1984-01-16
DK18084A (da) 1984-08-01

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