ES8606486A1 - Un procedimiento para preparar una cepa de bacillus subtilis - Google Patents

Un procedimiento para preparar una cepa de bacillus subtilis

Info

Publication number
ES8606486A1
ES8606486A1 ES543920A ES543920A ES8606486A1 ES 8606486 A1 ES8606486 A1 ES 8606486A1 ES 543920 A ES543920 A ES 543920A ES 543920 A ES543920 A ES 543920A ES 8606486 A1 ES8606486 A1 ES 8606486A1
Authority
ES
Spain
Prior art keywords
host
amylase
negative
vector system
bacillus subtilis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES543920A
Other languages
English (en)
Other versions
ES543920A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever Bestfoods North America
Original Assignee
Unilever Bestfoods North America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Bestfoods North America filed Critical Unilever Bestfoods North America
Publication of ES8606486A1 publication Critical patent/ES8606486A1/es
Publication of ES543920A0 publication Critical patent/ES543920A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UNA CEPA DE BACILUS SUBTILIS B1-109. COMPRENDE: A) TRANSFORMAR CELULAS COMPETENTES DE (A) QUE TIENEN CUATRO MARCADORES GENETICOS (F, G, H, I) CON ADN DE (B); B) AISLAR CELULAS TRANSFORMADAS QUE PRODUZCAN UNA ENZIMA A-AMILASA, PARA PRODUCIR A (C) QUE CONTIENEN LOS MARCADORES (G, H, I); C) TRANSFORMAR CELULAS COMPETENTES DE (C) CON ADN DE (D) Y D) AISLAR CELULAS TRANSFORMADORAS QUE SE DESARROLLEN EN AUSENCIA DE METIONINA AGREGADA, NO PRODUZCAN ENZIMA DE A-AMILASA Y QUE FORMEN ESPORAS CUANDO SE CALIENTAN A 90JC, DURANTE 10 MIN., PARA OBTENER A (E) QUE CONTIENEN MARCADURAS GENETICAS (J, E, I). SIENDO: (A) CEPA 1A289 DE (K); (B) CEPA 1A221 DE (K); (C) CEPA (K) B1-20; (D) CEPA 1S30 DE (K); (E) CEPA (K) B1-109; (F) AROI 906 UNA MUTACION DE REQUERIMIENTO DE FENILALAMINA O TIROSINA; (G) AMYE UNA DEFICIENCIA EN EL GEN ESTRUCTURAL DE PRODUCCION DE A-AMILASA; (H) METB5 UNA MUTACION DE REQUERIMIENTO DE METIONINA; (I) SACA321 UNA MUTACION DE INHIBICION DE FORMACION EN SUCROSA;(J) SPOIIA12, UNA MUTACION DE SUPRESION DE ESPORULACION Y (K) BACILLUS SUBTILIS.
ES543920A 1984-06-06 1985-06-05 Un procedimiento para preparar una cepa de bacillus subtilis Expired ES8606486A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US61787484A 1984-06-06 1984-06-06

Publications (2)

Publication Number Publication Date
ES8606486A1 true ES8606486A1 (es) 1986-04-01
ES543920A0 ES543920A0 (es) 1986-04-01

Family

ID=24475397

Family Applications (1)

Application Number Title Priority Date Filing Date
ES543920A Expired ES8606486A1 (es) 1984-06-06 1985-06-05 Un procedimiento para preparar una cepa de bacillus subtilis

Country Status (12)

Country Link
EP (1) EP0164117B1 (es)
JP (1) JPS611381A (es)
AT (1) ATE64755T1 (es)
AU (1) AU4308685A (es)
BR (1) BR8502586A (es)
CA (1) CA1288710C (es)
DE (1) DE3583310D1 (es)
DK (1) DK251385A (es)
ES (1) ES8606486A1 (es)
FI (1) FI81380C (es)
GB (1) GB2182042B (es)
IE (1) IE58099B1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1244477B (it) * 1990-12-21 1994-07-15 Eniricerche Spa Ceppo asporigeno di bacillus subtilis e suo impiego come ospite per la preparazione di prodotti eterologhi
US7981659B2 (en) 2005-10-13 2011-07-19 Kao Corporation Bacillus subtilis mutant strain
JP6019528B2 (ja) * 2012-06-05 2016-11-02 池田食研株式会社 発酵調味料の製造方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4465773A (en) * 1982-04-21 1984-08-14 Cpc International Inc. Amylase-negative, asporogenous mutant of Bacillus subtilis useful as a host in a host-vector system
US4450235A (en) * 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
US4450236A (en) * 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of Bacillus subtilis useful as a host in a host-vector system
EP0134048B2 (en) * 1983-07-06 1996-08-14 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species

Also Published As

Publication number Publication date
GB8526549D0 (en) 1985-12-04
JPS611381A (ja) 1986-01-07
IE851093L (en) 1985-12-06
FI81380B (fi) 1990-06-29
BR8502586A (pt) 1986-02-04
GB2182042B (en) 1989-10-18
DK251385D0 (da) 1985-06-04
FI81380C (fi) 1990-10-10
FI852038L (fi) 1985-12-07
DE3583310D1 (de) 1991-08-01
JPH0579308B2 (es) 1993-11-02
GB2182042A (en) 1987-05-07
FI852038A0 (fi) 1985-05-22
ES543920A0 (es) 1986-04-01
CA1288710C (en) 1991-09-10
ATE64755T1 (de) 1991-07-15
EP0164117A3 (en) 1987-08-19
DK251385A (da) 1985-12-07
AU4308685A (en) 1985-12-12
EP0164117A2 (en) 1985-12-11
EP0164117B1 (en) 1991-06-26
IE58099B1 (en) 1993-06-30

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970401