ES8602122A1 - Procedimiento de preparar un plasmidio circular - Google Patents

Procedimiento de preparar un plasmidio circular

Info

Publication number
ES8602122A1
ES8602122A1 ES534842A ES534842A ES8602122A1 ES 8602122 A1 ES8602122 A1 ES 8602122A1 ES 534842 A ES534842 A ES 534842A ES 534842 A ES534842 A ES 534842A ES 8602122 A1 ES8602122 A1 ES 8602122A1
Authority
ES
Spain
Prior art keywords
derived
natto
base sequence
plasmid
subtilis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534842A
Other languages
English (en)
Other versions
ES534842A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Publication of ES534842A0 publication Critical patent/ES534842A0/es
Publication of ES8602122A1 publication Critical patent/ES8602122A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEIDIENTO DE PREPARAR UN PLASMIDO CIRCULAR. COMPRENDE: A) ESCINDIR UN ACIDO DESOXIDORRIBONUCLEICO CROMOSOMICO DE BACILLUS SUBTILIS (NATTO) CONTENIENDO UN PROMOTOR ALFA-AMILASA CON UNA ENDONUCLEASA PARA FORMAR FRAGMENTOS DE ACIDO DESOXIDORRIBONUCLEICO; B) EMPALMAR LOS FRAGMENTOS DE ACIDO DESOXIDORRIBONUCLEICO A PLASMIDIOS CONOCIDOS Y TAMIZAR LOS PLASMIDIOS HIBRIDOS RESULTANTES USANDO REACCION ALMIDON-YODO PARA SELECCIONAR UN PLASMIDO QUE EXHIBE PROMOTOR AMILASA DERIVADO; Y C) EMPALMAR EL PLASMIDO QUE CONTIENE PROMOTOR DE ALFA-AMILASA A UN PLASMIDIO DERIVADO DE UN BACTERIO GRAMNEGATIVO. SE UTILIZA COMO VECTOR PLASMIDIAL QUE PERMITE UNA MANIFESTACION EFICIENTE DE GENES INTRODUCIDOS EN CELULAS HUESPED; PARA PROPORCIONAR UN VECTOR PLASMIDIAL CON ELEVADO NUMERO DE COPIAS; PROPORCIONAR UN VECTOR PLASMIDIAL QUE PERMITE LA PRODUCCION DE VARIAS PROTEINAS. PUEDE CRECER EN BACTERIAS GRAMPOSITIVAS Y GRAMNEGATIVAS.
ES534842A 1983-07-22 1984-07-20 Procedimiento de preparar un plasmidio circular Expired ES8602122A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58135032A JPS6027388A (ja) 1983-07-22 1983-07-22 プラスミツド

Publications (2)

Publication Number Publication Date
ES534842A0 ES534842A0 (es) 1985-12-01
ES8602122A1 true ES8602122A1 (es) 1985-12-01

Family

ID=15142345

Family Applications (1)

Application Number Title Priority Date Filing Date
ES534842A Expired ES8602122A1 (es) 1983-07-22 1984-07-20 Procedimiento de preparar un plasmidio circular

Country Status (4)

Country Link
EP (1) EP0135045B1 (es)
JP (1) JPS6027388A (es)
DE (1) DE3483760D1 (es)
ES (1) ES8602122A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61135599A (ja) * 1984-12-04 1986-06-23 Green Cross Corp:The 異種蛋白質の製造法
JPS61177996A (ja) * 1985-02-02 1986-08-09 Green Cross Corp:The インタ−フエロン−γの製造法
JPH0779698B2 (ja) * 1985-10-01 1995-08-30 萬有製薬株式会社 安全性の高い多機能クロ−ニングベクタ−
JPH07108224B2 (ja) * 1985-11-07 1995-11-22 重三 鵜高 バチルス・ブレビスを用いる遺伝子の発現方法
GB8704908D0 (en) * 1987-03-03 1987-04-08 Apcel Ltd Vectors
JPH0335105U (es) * 1989-08-15 1991-04-05
JPH0486835U (es) * 1990-12-06 1992-07-28
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统

Also Published As

Publication number Publication date
DE3483760D1 (de) 1991-01-31
ES534842A0 (es) 1985-12-01
JPS6027388A (ja) 1985-02-12
EP0135045A2 (en) 1985-03-27
EP0135045A3 (en) 1987-01-28
EP0135045B1 (en) 1990-12-19

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970519