ES8507265A1 - Un metodo de medir un ligando biologico - Google Patents

Un metodo de medir un ligando biologico

Info

Publication number
ES8507265A1
ES8507265A1 ES531979A ES531979A ES8507265A1 ES 8507265 A1 ES8507265 A1 ES 8507265A1 ES 531979 A ES531979 A ES 531979A ES 531979 A ES531979 A ES 531979A ES 8507265 A1 ES8507265 A1 ES 8507265A1
Authority
ES
Spain
Prior art keywords
ligand
hand
biotinyl enzyme
immobilised
biotinyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES531979A
Other languages
English (en)
Other versions
ES531979A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Publication of ES8507265A1 publication Critical patent/ES8507265A1/es
Publication of ES531979A0 publication Critical patent/ES531979A0/es
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y10S436/813—Cancer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

METODO DE MEDIR UN LIGANDO BIOLOGICO.COMPRENDE: DEJAR QUE COEXITAN UNA COMPOSICION CON ACTIVIDAD BIOLOGICA, QUE COMPRENDE UNA FASE DE INMOVILIZACION DE UN ANTICUERPO CAPAZ DE REACCIONAR CON EL LIGANDO (1) A MEDIR, O UN LIGANDO (2) BIOLOGICO CAPAZ DE REACCIONAR CON EL ANTICUERPO, Y UNA FASE DE INMOVILIZACION DE UNA ENZIMA BIOTINILADA O UN INHIBIDOR DE ENZIMA BIOTINILADA, UN CONJUGADO SOLUBLE EN AGUA DEL LIGANDO (2) O EL ANTICUERPO, Y UN INHIBIDOR DE ENZIMA BIOTINILADA, Y EL LIGANDO (1) A MEDIR EN SOLUCION ACUOSA; Y MEDIR LA ACTIVIDAD DE ENZIMA BIOTINILADA O LA ACTIVIDAD DE INHIBICION DE ENZIMA BIOTINILADA, DE LA COMPOSICION CON ACTIVIDAD BIOLOGICA O DE LA SOLUCION ACUOSA.
ES531979A 1983-04-30 1984-04-27 Un metodo de medir un ligando biologico Granted ES531979A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58077255A JPS59202064A (ja) 1983-04-30 1983-04-30 抗原決定基具有物質を測定する方法

Publications (2)

Publication Number Publication Date
ES8507265A1 true ES8507265A1 (es) 1985-08-16
ES531979A0 ES531979A0 (es) 1985-08-16

Family

ID=13628742

Family Applications (1)

Application Number Title Priority Date Filing Date
ES531979A Granted ES531979A0 (es) 1983-04-30 1984-04-27 Un metodo de medir un ligando biologico

Country Status (5)

Country Link
US (1) US4649105A (es)
EP (1) EP0124366B1 (es)
JP (1) JPS59202064A (es)
DE (1) DE3485215D1 (es)
ES (1) ES531979A0 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE107031T1 (de) * 1984-12-03 1994-06-15 Hoechst Celanese Corp Verfahren zur bestimmung eines liganden.
DK145385D0 (da) * 1985-03-29 1985-04-01 Novo Industri As Monoklonalt antistof til immunkemisk analyse
US5206144A (en) * 1985-03-29 1993-04-27 Novo Industri A/S Determination of glycated (glycosylated) hemoglobin in blood
WO1987006006A1 (en) * 1986-03-26 1987-10-08 Murex Corporation Anti-enzyme antibody immunoassay
US5206136A (en) * 1986-11-19 1993-04-27 Genetic Systems Corporation Rapid membrane affinity concentration assays
US5206178A (en) * 1986-11-19 1993-04-27 Genetic Systems Corporation Membrane affinity concentration immunoassay
US4904583A (en) * 1987-05-26 1990-02-27 Becton, Dickinson And Company Cascade immunoassay by multiple binding reactions
DE3852166T2 (de) * 1987-09-30 1995-04-06 Fuji Photo Film Co Ltd Analytische Vorrichtung für enzymimmunologische Tests.
US5362624A (en) * 1988-05-25 1994-11-08 Boehringer Mannheim Gmbh Process for the determination of an immunologically detectable substance and a suitable reaction vessel therefor
US5279937A (en) * 1992-04-30 1994-01-18 Detechnology Canada Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays
US5624813A (en) * 1994-04-21 1997-04-29 Mahant; Vijay K. NAD(P)+ /NAD(P)H based chemiluminescent diagnostics
US5916757A (en) * 1994-05-31 1999-06-29 Johnson & Johnson Clinical Diagnostics, Inc. Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies
US5705353A (en) * 1995-06-07 1998-01-06 Beckman Instruments, Inc. Method of reducing interferences in assays
US6825000B1 (en) * 1998-05-15 2004-11-30 Sekisui Chemical Co., Ltd. Immunoassay reagent and immunoassay method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4208479A (en) * 1977-07-14 1980-06-17 Syva Company Label modified immunoassays
GB2013211B (en) * 1978-01-26 1982-06-30 Technicon Instr Immunoassays using f(ab')2 fragments
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
AU8594382A (en) * 1981-08-05 1983-02-10 F. Hoffmann-La Roche Ag Immunoassay using biotin-avidin-enzyme complex
JPS58209994A (ja) * 1982-05-10 1983-12-07 Fujirebio Inc 活性蛋白等固定化物を用いた抗原の測定方法

Also Published As

Publication number Publication date
EP0124366A2 (en) 1984-11-07
EP0124366A3 (en) 1988-01-13
JPS59202064A (ja) 1984-11-15
EP0124366B1 (en) 1991-10-30
DE3485215D1 (de) 1991-12-05
JPH0340830B2 (es) 1991-06-20
US4649105A (en) 1987-03-10
ES531979A0 (es) 1985-08-16

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970519