ES8504458A1 - Procedimiento para producir la subunidad a la toxina del colera. - Google Patents

Procedimiento para producir la subunidad a la toxina del colera.

Info

Publication number
ES8504458A1
ES8504458A1 ES522566A ES522566A ES8504458A1 ES 8504458 A1 ES8504458 A1 ES 8504458A1 ES 522566 A ES522566 A ES 522566A ES 522566 A ES522566 A ES 522566A ES 8504458 A1 ES8504458 A1 ES 8504458A1
Authority
ES
Spain
Prior art keywords
dna
units
preparation
unit
compositions containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES522566A
Other languages
English (en)
Other versions
ES522566A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline RIT
Original Assignee
SmithKline RIT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline RIT filed Critical SmithKline RIT
Publication of ES522566A0 publication Critical patent/ES522566A0/es
Publication of ES8504458A1 publication Critical patent/ES8504458A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/909—Vibrio

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR LA SUBUNIDAD A DE LA TOXINA DEL COLERA.CONSISTE EN: A) PROPORCIONAR UNA MOLECULA DE ADN RECOMBINANTE QUE CONTIENE UNA SECUENCIA DE ADN QUE COMPRENDE UNA PORCION QUE CODIFICA LA TOTALIDAD O PARTE DE LA SUBUNIDAD A DE LA TOXINA DEL COLERA MEDIANTE DIGESTION DE ADN DE UNA CEPA DE V. CHOLERAE, PRODUCTORA DE LA SUBUNIDAD A DE LA TOXINA DEL COLERA CON LA ENDONUCLEASA CLAI; B) AISLAR UN FRAGMENTO QUE TIENE HIBRIDACION ESPECIFICA A UNA SONDA ELTA; C) MEZCLAR DICHO FRAGMENTO CON UN VECTOR QUE TIENE UN SITIO DE RESTRICCION POR ENDONUCLEASA CLAI Y PREVIAMENTE DIGERIDO POR DICHA CLAI E INSERTAR EL FRAGMENTO; D) TRANSFORMAR UN ORGANISMO HOSPEDANTE APROPIADO CON DICHA MOLECULA DE ADN RECOMBINANTE; E) CULTIVAR EL ORGANISMO HOSPEDANTE TRANSFORMADO; Y F) RECOGER DICHA SUBUNIDAD A DE LA TOXINA DEL COLERA.
ES522566A 1982-05-24 1983-05-20 Procedimiento para producir la subunidad a la toxina del colera. Expired ES8504458A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/381,083 US4666837A (en) 1982-05-24 1982-05-24 DNA sequences, recombinant DNA molecules and processes for producing the A and B subunits of cholera toxin and preparations containing so-obtained subunit or subunits

Publications (2)

Publication Number Publication Date
ES522566A0 ES522566A0 (es) 1985-04-16
ES8504458A1 true ES8504458A1 (es) 1985-04-16

Family

ID=23503587

Family Applications (3)

Application Number Title Priority Date Filing Date
ES522566A Expired ES8504458A1 (es) 1982-05-24 1983-05-20 Procedimiento para producir la subunidad a la toxina del colera.
ES529276A Expired ES8504460A1 (es) 1982-05-24 1984-01-30 Procedimiento para producir la totalidad o parte de las subunidades a y b de la toxina del colera
ES535809A Granted ES535809A0 (es) 1982-05-24 1984-09-11 Procedimiento para producir la subunidad b de la toxina del colera

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES529276A Expired ES8504460A1 (es) 1982-05-24 1984-01-30 Procedimiento para producir la totalidad o parte de las subunidades a y b de la toxina del colera
ES535809A Granted ES535809A0 (es) 1982-05-24 1984-09-11 Procedimiento para producir la subunidad b de la toxina del colera

Country Status (10)

Country Link
US (1) US4666837A (es)
EP (1) EP0095452A3 (es)
JP (1) JPS58222033A (es)
AU (1) AU1348083A (es)
CA (1) CA1212640A (es)
DK (1) DK211483A (es)
ES (3) ES8504458A1 (es)
GR (1) GR79261B (es)
PT (1) PT76726B (es)
ZA (1) ZA833662B (es)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882653A (en) * 1983-03-04 1999-03-16 The University Of Maryland System Vibrio cholerae 01 (CVD111) and non-01 (CVD112 and CVD112RM) serogroup vaccine strains, methods of making same and products thereof
US4935364A (en) * 1983-03-04 1990-06-19 Swiss Serum And Vaccine Institute Berne Method of isolating restriction fragment deletions in vibrio cholerae and products thereof
US5470729A (en) * 1983-03-04 1995-11-28 University Of Maryland At Baltimore Method of isolating restriction fragment deletions in Vibrio cholerae, and products thereof
US5399494A (en) * 1983-03-04 1995-03-21 The University Of Maryland System Vibrio cholerae strain CVD103Hgr, method of making same, and vaccines derived therefrom
US4882278A (en) * 1983-04-29 1989-11-21 President And Fellows Of Harvard College Non-toxinogenic vibrio cholerae mutants
GB8333131D0 (en) * 1983-12-12 1984-01-18 Glaxo Group Ltd Microbiological process
AU589114B2 (en) * 1984-02-01 1989-10-05 Enterovax Limited Genetically engineered bacteria useful as a vaccine
US5668255A (en) * 1984-06-07 1997-09-16 Seragen, Inc. Hybrid molecules having translocation region and cell-binding region
US6022950A (en) * 1984-06-07 2000-02-08 Seragen, Inc. Hybrid molecules having translocation region and cell-binding region
CA1340372C (en) * 1984-07-09 1999-02-02 John D. Clements Production of e. coli lt-b enterotoxin subunit
US5308835A (en) * 1984-07-09 1994-05-03 Praxis Biologics, Inc. Production of the E. coli LT-B enterotoxin subunit
US4808700A (en) * 1984-07-09 1989-02-28 Praxis Biologics, Inc. Immunogenic conjugates of non-toxic E. coli LT-B enterotoxin subunit and capsular polymers
US5258498A (en) * 1987-05-21 1993-11-02 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
DE3853515T3 (de) * 1987-05-21 2005-08-25 Micromet Ag Multifunktionelle proteine mit vorbestimmter zielsetzung.
US5132405A (en) * 1987-05-21 1992-07-21 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5091513A (en) * 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US6043057A (en) * 1988-09-16 2000-03-28 Vitec Aktiebolag Recombinant systems for expression of the cholera B-sub-unit with the aid of foreign promoters and/or leader peptides
SE462285B (sv) * 1988-09-16 1990-05-28 Jan Roland Holmgren Expression av koleratoxinets bindande subenhet med hjaelp av fraemmande promotor- och/eller ledarpeptidstrukturer
FR2636842B1 (fr) * 1988-09-27 1994-06-10 Liege Universite Etat Proteine de fusion d'une sequence derivee de la sous-unite b de la toxine cholerique et d'un antigene heterologue doue de proprietes immunogenes, compositions de vaccins les contenant et acides nucleiques recombinants contenant une sequence de nucleotides codant pour ladite proteine de fusion
US20040014068A1 (en) * 1988-10-05 2004-01-22 Whatman, Inc. Solid medium and method for DNA storage
US5985327A (en) * 1988-10-05 1999-11-16 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
JPH05502880A (ja) * 1989-12-22 1993-05-20 セラジェン・インコーポレーテッド トランスロケーション領域および細胞結合領域を有するハイブリッド分子
IL101715A (en) * 1991-05-02 2005-06-19 Amgen Inc Recombinant dna-derived cholera toxin subunit analogs
IT1253009B (it) * 1991-12-31 1995-07-10 Sclavo Ricerca S R L Mutanti immunogenici detossificati della tossina colerica e della tossina lt, loro preparazione ed uso per la preparazione di vaccini
GB9513371D0 (en) * 1995-06-30 1995-09-06 Biocine Spa Immunogenic detoxified mutant toxins
USH1985H1 (en) 1992-01-09 2001-08-07 The United States Of America As Represented By The Secretary Of The Navy Method for detecting biological toxins
JP3134907B2 (ja) * 1993-06-15 2001-02-13 株式会社島津製作所 コレラ菌検出用オリゴヌクレオチドおよびそれを用いた検出法
GB9326174D0 (en) * 1993-12-22 1994-02-23 Biocine Sclavo Mucosal adjuvant
US5773003A (en) * 1995-03-31 1998-06-30 Immulogic, Inc. Hapten-carrier conjugates for use in drug-abuse therapy and methods for preparation of same
US20020032316A1 (en) * 1995-03-31 2002-03-14 Cantab Pharmaceuticals Research Limited Hapten-carrier conjugates for use in drug-abuse therapy and methods for preparation of same
ATE254930T1 (de) * 1995-03-31 2003-12-15 Xenova Res Ltd Hapten-träger-konjugate zur anwendung in der drogen-missbrauchs-therapie
US6818222B1 (en) 1997-03-21 2004-11-16 Chiron Corporation Detoxified mutants of bacterial ADP-ribosylating toxins as parenteral adjuvants
US7097845B2 (en) 1997-04-23 2006-08-29 Jacob Sten Petersen Combinations of antigen and mucosal binding component for inducing specific immunological tolerance
US6232082B1 (en) 1998-12-01 2001-05-15 Nabi Hapten-carrier conjugates for treating and preventing nicotine addiction
US20030162733A1 (en) * 2000-11-27 2003-08-28 Haynes Joel R. Nucleic acid adjuvants
AU2002252378B2 (en) 2001-03-19 2007-10-18 Intercell Usa, Inc. Transcutaneous immunostimulation
US20060099229A1 (en) * 2004-11-05 2006-05-11 Universiti Sains Malaysia Vibrio cholerae strains VCUSM1 and VCUSM4, method of producing same, and vaccine derivatives thereof
WO2014044690A1 (en) 2012-09-18 2014-03-27 Valneva Austria Gmbh Improved vaccines

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
US4264737A (en) * 1978-09-21 1981-04-28 President And Fellows Of Harvard College Method of making genetically stable mutants of vibrio cholerae
US4332892A (en) * 1979-01-15 1982-06-01 President And Fellows Of Harvard College Protein synthesis
US4328209A (en) * 1979-04-11 1982-05-04 Board Of Regents, The University Of Texas System Cholera vaccine
US4293652A (en) * 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis

Also Published As

Publication number Publication date
ES522566A0 (es) 1985-04-16
ES8506348A1 (es) 1985-07-01
US4666837A (en) 1987-05-19
EP0095452A3 (en) 1985-10-02
DK211483A (da) 1983-11-25
DK211483D0 (da) 1983-05-11
ES535809A0 (es) 1985-07-01
EP0095452A2 (fr) 1983-11-30
CA1212640A (en) 1986-10-14
AU1348083A (en) 1983-12-01
JPS58222033A (ja) 1983-12-23
GR79261B (es) 1984-10-22
PT76726B (en) 1985-12-27
ZA833662B (en) 1984-03-28
PT76726A (en) 1983-06-01
ES529276A0 (es) 1985-05-01
ES8504460A1 (es) 1985-05-01

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