ES8404858A1 - Method of affinity purification employing monoclonal antibodies. - Google Patents

Method of affinity purification employing monoclonal antibodies.

Info

Publication number
ES8404858A1
ES8404858A1 ES521371A ES521371A ES8404858A1 ES 8404858 A1 ES8404858 A1 ES 8404858A1 ES 521371 A ES521371 A ES 521371A ES 521371 A ES521371 A ES 521371A ES 8404858 A1 ES8404858 A1 ES 8404858A1
Authority
ES
Spain
Prior art keywords
monoclonal antibodies
affinity purification
antibodies
antigens
environment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES521371A
Other languages
Spanish (es)
Other versions
ES521371A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybritech Inc
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Publication of ES521371A0 publication Critical patent/ES521371A0/en
Publication of ES8404858A1 publication Critical patent/ES8404858A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Process for affinity purification of antigens and antibodies that employs monoclonal antibodies having a high affinity for the antigen in a first environment and a low affinity in a second environment, the environments being ones in which the immuno-chemical properties of the antigens and antibodies are not adversely affected. Also described is a process for fractionating antisera to obtain an antibody fraction having similar antigen binding properties as exhibited by the environmentally sensitive monoclonal antibodies. The effect of varying pH in the binding capability of tow monoclonal antibodies for human growth hormone is shown in fig. 1.
ES521371A 1982-04-12 1983-04-11 Method of affinity purification employing monoclonal antibodies. Expired ES8404858A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36778182A 1982-04-12 1982-04-12

Publications (2)

Publication Number Publication Date
ES521371A0 ES521371A0 (en) 1984-05-16
ES8404858A1 true ES8404858A1 (en) 1984-05-16

Family

ID=23448565

Family Applications (3)

Application Number Title Priority Date Filing Date
ES521371A Expired ES8404858A1 (en) 1982-04-12 1983-04-11 Method of affinity purification employing monoclonal antibodies.
ES528000A Expired ES8506902A1 (en) 1982-04-12 1983-12-13 Method of affinity purification employing monoclonal antibodies.
ES528001A Expired ES8706964A1 (en) 1982-04-12 1983-12-13 Method of affinity purification employing monoclonal antibodies.

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES528000A Expired ES8506902A1 (en) 1982-04-12 1983-12-13 Method of affinity purification employing monoclonal antibodies.
ES528001A Expired ES8706964A1 (en) 1982-04-12 1983-12-13 Method of affinity purification employing monoclonal antibodies.

Country Status (11)

Country Link
EP (1) EP0105359A4 (en)
AT (1) AT393386B (en)
AU (1) AU622099B2 (en)
CA (1) CA1209907A (en)
CH (1) CH672028A5 (en)
DK (1) DK192191A (en)
ES (3) ES8404858A1 (en)
FI (1) FI88403C (en)
GB (1) GB2128630B (en)
IT (1) IT1219779B (en)
WO (1) WO1983003678A1 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8328918D0 (en) * 1983-10-28 1983-11-30 Unilever Plc Alkaline phosphatase
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna
US4666865A (en) * 1984-01-13 1987-05-19 Centocor, Inc. Immunoassay for biologically active human interferon-gamma employing unique monoclonal antibodies
US4789631A (en) * 1984-02-17 1988-12-06 Synbiotics Corporation Immunoassay for anti-dirofilaria immitis antibody
GB8426468D0 (en) * 1984-10-19 1984-11-28 Technology Licence Co Ltd Monoclonal antibodies
AU5259786A (en) * 1985-01-30 1986-08-07 United States Of America, The Monoclonal antibodies against chlamydial genes specific lipopolysaccharide
US4859611A (en) * 1985-02-28 1989-08-22 Massachusetts Institute Of Technology Affinity column and process for detection of low molecular weight toxic substances
US4818687A (en) * 1985-02-28 1989-04-04 Massachusetts Institute Of Technology Affinity column and process for detection of low molecular weight toxic substances
IL75828A (en) * 1985-07-17 1991-06-10 Univ Ramot Immobilization by biologically active proteins
NZ218336A (en) * 1985-12-09 1991-08-27 Kirin Amgen Inc Monoclonal antibodies to human pluripotent granulocyte colony stimulating factor (hpg-csf)
US5175255A (en) * 1987-03-23 1992-12-29 Amgen Inc. Methods for purification of platelet-derived growth factor
US5190859A (en) * 1987-02-26 1993-03-02 Dana-Farber Cancer Institute, Inc. Purification of LFA-3
SE468653B (en) * 1987-03-06 1993-02-22 Perstorp Biolytica ADSORBENT FOR ISOCRATIC AFFINITY CHROMATOGRAPHY
JPS6463599A (en) * 1987-05-01 1989-03-09 Xoma Corp Purification of immunoglobulin by selective anti-idiotype bond
EP0371049B1 (en) * 1987-07-14 1992-06-03 The Victoria University Of Manchester Method for the diagnosis of infections with detection of lipopolysaccharide antigens
CA1323567C (en) * 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies
US5582862A (en) * 1988-04-04 1996-12-10 General Hospital Corporation Antibodies that bind to α2-antiplasmin crosslinked to fibrin which do not inhibit plasma α2-antiplasmin
US5372812A (en) * 1988-04-04 1994-12-13 The General Hospital Corporation Composition and method for acceleration of clot lysis
WO1998012329A2 (en) * 1996-09-20 1998-03-26 The General Hospital Corporation Composition and method for enhancing fibrinolysis using antibodies to alpha-2-antiplasmin
DK2275443T3 (en) * 2008-04-11 2016-02-08 Chugai Pharmaceutical Co Ltd Antigen-binding molecule capable of repetitively binding to two or more antigen molecules
KR102385507B1 (en) 2010-11-30 2022-04-12 추가이 세이야쿠 가부시키가이샤 Antigen-binding molecule capable of binding to plurality of antigen molecules repeatedly
CN103492565B (en) 2011-02-25 2021-01-29 中外制药株式会社 Fc gamma RIIb specific Fc antibodies
EP3939996A1 (en) 2011-09-30 2022-01-19 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities
US20150056182A1 (en) 2011-11-30 2015-02-26 Chugai Seiyaku Kabushiki Kaisha Drug containing carrier into cell for forming immune complex
SG10201710322VA (en) 2014-12-19 2018-02-27 Chugai Pharmaceutical Co Ltd Anti-c5 antibodies and methods of use
TWI831538B (en) 2014-12-19 2024-02-01 日商中外製藥股份有限公司 ANTI-MYOSTATIN ANTIBODIES, POLYPEPTIDES CONTAINING VARIANT Fc REGIONs, AND METHODS OF USE
EP3394098A4 (en) 2015-12-25 2019-11-13 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies and methods of use
IL264626B (en) 2016-08-05 2022-07-01 Chugai Pharmaceutical Co Ltd Composition for prophylaxis or treatment of il-8 related diseases
WO2018139623A1 (en) 2017-01-30 2018-08-02 Chugai Seiyaku Kabushiki Kaisha Anti-sclerostin antibodies and methods of use
CN110724204B (en) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Method for purifying Fc fusion protein

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4172124A (en) * 1978-04-28 1979-10-23 The Wistar Institute Method of producing tumor antibodies
EP0059598B1 (en) * 1981-02-26 1985-07-17 Unilever Plc A process and apparatus for the recovery of immunoglobulines
US4474893A (en) * 1981-07-01 1984-10-02 The University of Texas System Cancer Center Recombinant monoclonal antibodies
US4361509A (en) * 1981-12-14 1982-11-30 Scripps Clinic And Research Foundation Ultrapurification of factor VIII using monoclonal antibodies

Also Published As

Publication number Publication date
ATA901983A (en) 1991-03-15
FI88403B (en) 1993-01-29
DK192191D0 (en) 1991-11-26
GB2128630A (en) 1984-05-02
ES528001A0 (en) 1987-07-01
ES528000A0 (en) 1985-08-01
CH672028A5 (en) 1989-10-13
GB8332644D0 (en) 1984-01-11
ES521371A0 (en) 1984-05-16
ES8706964A1 (en) 1987-07-01
FI834530A (en) 1983-12-09
EP0105359A4 (en) 1984-08-20
AU622099B2 (en) 1992-04-02
IT8320550A0 (en) 1983-04-12
ES8506902A1 (en) 1985-08-01
FI88403C (en) 1993-05-10
GB2128630B (en) 1986-08-28
FI834530A0 (en) 1983-12-09
IT1219779B (en) 1990-05-24
DK192191A (en) 1991-11-26
CA1209907A (en) 1986-08-19
AU7256987A (en) 1987-09-03
AT393386B (en) 1991-10-10
WO1983003678A1 (en) 1983-10-27
EP0105359A1 (en) 1984-04-18

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970401