ES8404858A1 - Method of affinity purification employing monoclonal antibodies. - Google Patents
Method of affinity purification employing monoclonal antibodies.Info
- Publication number
- ES8404858A1 ES8404858A1 ES521371A ES521371A ES8404858A1 ES 8404858 A1 ES8404858 A1 ES 8404858A1 ES 521371 A ES521371 A ES 521371A ES 521371 A ES521371 A ES 521371A ES 8404858 A1 ES8404858 A1 ES 8404858A1
- Authority
- ES
- Spain
- Prior art keywords
- monoclonal antibodies
- affinity purification
- antibodies
- antigens
- environment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000001261 affinity purification Methods 0.000 title abstract 2
- 239000000427 antigen Substances 0.000 abstract 4
- 102000036639 antigens Human genes 0.000 abstract 4
- 108091007433 antigens Proteins 0.000 abstract 4
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 230000002411 adverse Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000000984 immunochemical effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Process for affinity purification of antigens and antibodies that employs monoclonal antibodies having a high affinity for the antigen in a first environment and a low affinity in a second environment, the environments being ones in which the immuno-chemical properties of the antigens and antibodies are not adversely affected. Also described is a process for fractionating antisera to obtain an antibody fraction having similar antigen binding properties as exhibited by the environmentally sensitive monoclonal antibodies. The effect of varying pH in the binding capability of tow monoclonal antibodies for human growth hormone is shown in fig. 1.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36778182A | 1982-04-12 | 1982-04-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES521371A0 ES521371A0 (en) | 1984-05-16 |
ES8404858A1 true ES8404858A1 (en) | 1984-05-16 |
Family
ID=23448565
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES521371A Expired ES8404858A1 (en) | 1982-04-12 | 1983-04-11 | Method of affinity purification employing monoclonal antibodies. |
ES528000A Expired ES8506902A1 (en) | 1982-04-12 | 1983-12-13 | Method of affinity purification employing monoclonal antibodies. |
ES528001A Expired ES8706964A1 (en) | 1982-04-12 | 1983-12-13 | Method of affinity purification employing monoclonal antibodies. |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES528000A Expired ES8506902A1 (en) | 1982-04-12 | 1983-12-13 | Method of affinity purification employing monoclonal antibodies. |
ES528001A Expired ES8706964A1 (en) | 1982-04-12 | 1983-12-13 | Method of affinity purification employing monoclonal antibodies. |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0105359A4 (en) |
AT (1) | AT393386B (en) |
AU (1) | AU622099B2 (en) |
CA (1) | CA1209907A (en) |
CH (1) | CH672028A5 (en) |
DK (1) | DK192191A (en) |
ES (3) | ES8404858A1 (en) |
FI (1) | FI88403C (en) |
GB (1) | GB2128630B (en) |
IT (1) | IT1219779B (en) |
WO (1) | WO1983003678A1 (en) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8328918D0 (en) * | 1983-10-28 | 1983-11-30 | Unilever Plc | Alkaline phosphatase |
GB8331071D0 (en) * | 1983-11-22 | 1983-12-29 | Karayiannis P | Assay for dna/rna |
US4666865A (en) * | 1984-01-13 | 1987-05-19 | Centocor, Inc. | Immunoassay for biologically active human interferon-gamma employing unique monoclonal antibodies |
US4789631A (en) * | 1984-02-17 | 1988-12-06 | Synbiotics Corporation | Immunoassay for anti-dirofilaria immitis antibody |
GB8426468D0 (en) * | 1984-10-19 | 1984-11-28 | Technology Licence Co Ltd | Monoclonal antibodies |
AU5259786A (en) * | 1985-01-30 | 1986-08-07 | United States Of America, The | Monoclonal antibodies against chlamydial genes specific lipopolysaccharide |
US4859611A (en) * | 1985-02-28 | 1989-08-22 | Massachusetts Institute Of Technology | Affinity column and process for detection of low molecular weight toxic substances |
US4818687A (en) * | 1985-02-28 | 1989-04-04 | Massachusetts Institute Of Technology | Affinity column and process for detection of low molecular weight toxic substances |
IL75828A (en) * | 1985-07-17 | 1991-06-10 | Univ Ramot | Immobilization by biologically active proteins |
NZ218336A (en) * | 1985-12-09 | 1991-08-27 | Kirin Amgen Inc | Monoclonal antibodies to human pluripotent granulocyte colony stimulating factor (hpg-csf) |
US5175255A (en) * | 1987-03-23 | 1992-12-29 | Amgen Inc. | Methods for purification of platelet-derived growth factor |
US5190859A (en) * | 1987-02-26 | 1993-03-02 | Dana-Farber Cancer Institute, Inc. | Purification of LFA-3 |
SE468653B (en) * | 1987-03-06 | 1993-02-22 | Perstorp Biolytica | ADSORBENT FOR ISOCRATIC AFFINITY CHROMATOGRAPHY |
JPS6463599A (en) * | 1987-05-01 | 1989-03-09 | Xoma Corp | Purification of immunoglobulin by selective anti-idiotype bond |
EP0371049B1 (en) * | 1987-07-14 | 1992-06-03 | The Victoria University Of Manchester | Method for the diagnosis of infections with detection of lipopolysaccharide antigens |
CA1323567C (en) * | 1987-10-05 | 1993-10-26 | Gene R. Nathans | Method for purification of antibodies |
US5582862A (en) * | 1988-04-04 | 1996-12-10 | General Hospital Corporation | Antibodies that bind to α2-antiplasmin crosslinked to fibrin which do not inhibit plasma α2-antiplasmin |
US5372812A (en) * | 1988-04-04 | 1994-12-13 | The General Hospital Corporation | Composition and method for acceleration of clot lysis |
WO1998012329A2 (en) * | 1996-09-20 | 1998-03-26 | The General Hospital Corporation | Composition and method for enhancing fibrinolysis using antibodies to alpha-2-antiplasmin |
DK2275443T3 (en) * | 2008-04-11 | 2016-02-08 | Chugai Pharmaceutical Co Ltd | Antigen-binding molecule capable of repetitively binding to two or more antigen molecules |
KR102385507B1 (en) | 2010-11-30 | 2022-04-12 | 추가이 세이야쿠 가부시키가이샤 | Antigen-binding molecule capable of binding to plurality of antigen molecules repeatedly |
CN103492565B (en) | 2011-02-25 | 2021-01-29 | 中外制药株式会社 | Fc gamma RIIb specific Fc antibodies |
EP3939996A1 (en) | 2011-09-30 | 2022-01-19 | Chugai Seiyaku Kabushiki Kaisha | Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities |
US20150056182A1 (en) | 2011-11-30 | 2015-02-26 | Chugai Seiyaku Kabushiki Kaisha | Drug containing carrier into cell for forming immune complex |
SG10201710322VA (en) | 2014-12-19 | 2018-02-27 | Chugai Pharmaceutical Co Ltd | Anti-c5 antibodies and methods of use |
TWI831538B (en) | 2014-12-19 | 2024-02-01 | 日商中外製藥股份有限公司 | ANTI-MYOSTATIN ANTIBODIES, POLYPEPTIDES CONTAINING VARIANT Fc REGIONs, AND METHODS OF USE |
EP3394098A4 (en) | 2015-12-25 | 2019-11-13 | Chugai Seiyaku Kabushiki Kaisha | Anti-myostatin antibodies and methods of use |
IL264626B (en) | 2016-08-05 | 2022-07-01 | Chugai Pharmaceutical Co Ltd | Composition for prophylaxis or treatment of il-8 related diseases |
WO2018139623A1 (en) | 2017-01-30 | 2018-08-02 | Chugai Seiyaku Kabushiki Kaisha | Anti-sclerostin antibodies and methods of use |
CN110724204B (en) * | 2019-11-18 | 2021-10-22 | 广东菲鹏制药股份有限公司 | Method for purifying Fc fusion protein |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4172124A (en) * | 1978-04-28 | 1979-10-23 | The Wistar Institute | Method of producing tumor antibodies |
EP0059598B1 (en) * | 1981-02-26 | 1985-07-17 | Unilever Plc | A process and apparatus for the recovery of immunoglobulines |
US4474893A (en) * | 1981-07-01 | 1984-10-02 | The University of Texas System Cancer Center | Recombinant monoclonal antibodies |
US4361509A (en) * | 1981-12-14 | 1982-11-30 | Scripps Clinic And Research Foundation | Ultrapurification of factor VIII using monoclonal antibodies |
-
1983
- 1983-04-11 ES ES521371A patent/ES8404858A1/en not_active Expired
- 1983-04-11 CA CA000425555A patent/CA1209907A/en not_active Expired
- 1983-04-12 WO PCT/US1983/000524 patent/WO1983003678A1/en not_active Application Discontinuation
- 1983-04-12 GB GB08332644A patent/GB2128630B/en not_active Expired
- 1983-04-12 CH CH6699/83A patent/CH672028A5/de not_active IP Right Cessation
- 1983-04-12 IT IT20550/83A patent/IT1219779B/en active
- 1983-04-12 AT AT9019/83A patent/AT393386B/en not_active IP Right Cessation
- 1983-04-12 EP EP19830901671 patent/EP0105359A4/en not_active Withdrawn
- 1983-12-09 FI FI834530A patent/FI88403C/en not_active IP Right Cessation
- 1983-12-13 ES ES528000A patent/ES8506902A1/en not_active Expired
- 1983-12-13 ES ES528001A patent/ES8706964A1/en not_active Expired
-
1987
- 1987-05-06 AU AU72569/87A patent/AU622099B2/en not_active Ceased
-
1991
- 1991-11-26 DK DK192191A patent/DK192191A/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
ATA901983A (en) | 1991-03-15 |
FI88403B (en) | 1993-01-29 |
DK192191D0 (en) | 1991-11-26 |
GB2128630A (en) | 1984-05-02 |
ES528001A0 (en) | 1987-07-01 |
ES528000A0 (en) | 1985-08-01 |
CH672028A5 (en) | 1989-10-13 |
GB8332644D0 (en) | 1984-01-11 |
ES521371A0 (en) | 1984-05-16 |
ES8706964A1 (en) | 1987-07-01 |
FI834530A (en) | 1983-12-09 |
EP0105359A4 (en) | 1984-08-20 |
AU622099B2 (en) | 1992-04-02 |
IT8320550A0 (en) | 1983-04-12 |
ES8506902A1 (en) | 1985-08-01 |
FI88403C (en) | 1993-05-10 |
GB2128630B (en) | 1986-08-28 |
FI834530A0 (en) | 1983-12-09 |
IT1219779B (en) | 1990-05-24 |
DK192191A (en) | 1991-11-26 |
CA1209907A (en) | 1986-08-19 |
AU7256987A (en) | 1987-09-03 |
AT393386B (en) | 1991-10-10 |
WO1983003678A1 (en) | 1983-10-27 |
EP0105359A1 (en) | 1984-04-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES8706964A1 (en) | Method of affinity purification employing monoclonal antibodies. | |
ES8206856A1 (en) | Method for the determination of antigens with the aid of two or more monoclonal antibodies. | |
AU1964883A (en) | Monoclonal antibody immunoassay | |
ES2106195T3 (en) | IGG AGLYCOSILATED ANTIBODY ANTI CD3. | |
EP0098162A3 (en) | Monoclonal antibodies specific to carcinoembryonic antigen | |
DK0432249T3 (en) | Monoclonal antibodies to the beta chain of the leukocyte adhesion receptor, methods for producing these antibodies and their use | |
MY101457A (en) | Toxins and antibodies of c.difficile. | |
AU564575B2 (en) | Polyvalent endotoxin specific antibodies | |
AU567681B2 (en) | Immunoassay involving monoclonal igm antibody | |
MY100328A (en) | Toxins and antibodies of c.difficile | |
EP0287397A3 (en) | Monoclonal antibody specific to human pancreatic phospholipase a2 | |
CA2054689A1 (en) | An immunoassay method for determining the specificity of binding of a monoclonal antibody to an antigen | |
ES8401261A1 (en) | Procedure for immunological determinations using two antibodies, the second antibody being purified by affinity chromatography, and supports therefor. | |
ES8707563A1 (en) | Its production and use. | |
EP0186371A3 (en) | Monoclonal antibodies specific to antigens of hepatitis b virus | |
AU2900184A (en) | Membrane antigens generated from pooled cells and monoclonal antibodies | |
EP0089771A3 (en) | Atcc hb8116 and its monoclonal anti-h-y antibody, hyclonalan | |
AU2805884A (en) | Monoclonal antibodies against tumor antigens and method | |
DE3174878D1 (en) | Immunoassay utilizing monoclonal high affinity igm antibodies | |
AU1550783A (en) | Method of affinity purification employing monoclonal antibodies | |
CA2178504A1 (en) | Immunoassays for prostate specific antigen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970401 |