ES8107311A1 - Un metodo para detectar sangre oculta en un especimen huma- no. - Google Patents

Un metodo para detectar sangre oculta en un especimen huma- no.

Info

Publication number
ES8107311A1
ES8107311A1 ES497544A ES497544A ES8107311A1 ES 8107311 A1 ES8107311 A1 ES 8107311A1 ES 497544 A ES497544 A ES 497544A ES 497544 A ES497544 A ES 497544A ES 8107311 A1 ES8107311 A1 ES 8107311A1
Authority
ES
Spain
Prior art keywords
aqueous
membrane
occult blood
disclosed
quantifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES497544A
Other languages
English (en)
Other versions
ES497544A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
Original Assignee
Baylor College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
Publication of ES497544A0 publication Critical patent/ES497544A0/es
Publication of ES8107311A1 publication Critical patent/ES8107311A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/725—Haemoglobin using peroxidative activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO PARA DETECTAR HEMOGLOBINA EN ESPECIMENES HUMANOS, COMO HECES Y ORINA. SE REALIZA UNA SUSPENSION AL 1 POR 100 DE HECES EN UNA SOLUCION ACUOSA TAMPON DE GLICINA. SE TRATA CON LISOZIMA, EN LA CANTIDAD DE 1 MG/ML, PARA DIGERIR LOS POLISACARIDOS PRESENTES. SE ACIDIFICA ESTA MUESTRA CON ACIDO ACETICO O CITRICO EN UNA PROPORCION DEL 1 POR 100. SE SEPARA EN PORCIONES LIQUIDA Y SOLIDA, HACIENDOLA PASAR POR UN CLASIFICADOR ELECTROPOSITIVO CON POROSIDAD ENTRE 0,65 Y 2 MICRAS. EL LIQUIDO CLARIFICADO SE PASA POR UNA MEMBRANA ELECTRONEGATIVA, CON POROSIDAD DE 1 MICRA, CAPAZ DE ABSORBER HEMOGLOBINA. SE LAVA LA MEMBRANA CON UNA COMPOSICION DE ACIDO ACETICO AL 1 POR 100 PARA RETIRAR LOS FLUIDOS FECALES RESIDUALES. SE TRATA LA MEMBRANA CON UN INDICADOR DE PEROXIDASA A BASE DE ORTO-TOLIDINA Y PEROXIDO DE HIDROGENO. COMPARANDO EL COLOR CON UN PATRON DE COLORES DE CANTIDADES CONOCIDAS DE HEMOGLOBINA SE DETERMINA LA CANTIDAD DE HEMOGLOBINA. EN EL CASO DE LA ORINA NO ES NECESARIA LA SUSPENSION Y DIGESTIONPOR ENZIMA PARA PREPARAR LA MUESTRA. ESTE METODO SE UTILIZA PARA DIAGNOSTICO PRECOZ DE CANCER DE COLON.
ES497544A 1979-12-10 1980-12-09 Un metodo para detectar sangre oculta en un especimen huma- no. Expired ES8107311A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/101,722 US4277250A (en) 1979-12-10 1979-12-10 Methods for detecting and quantifying occult blood

Publications (2)

Publication Number Publication Date
ES497544A0 ES497544A0 (es) 1981-09-16
ES8107311A1 true ES8107311A1 (es) 1981-09-16

Family

ID=22286065

Family Applications (1)

Application Number Title Priority Date Filing Date
ES497544A Expired ES8107311A1 (es) 1979-12-10 1980-12-09 Un metodo para detectar sangre oculta en un especimen huma- no.

Country Status (6)

Country Link
US (1) US4277250A (es)
EP (1) EP0030388A3 (es)
JP (1) JPS5693045A (es)
BR (1) BR8008073A (es)
ES (1) ES8107311A1 (es)
NO (1) NO803479L (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4333734A (en) * 1980-01-18 1982-06-08 Sloan-Kettering Institute For Cancer Research Diagnostic device for fecal occult blood and method of use
US4956300A (en) * 1982-01-05 1990-09-11 Helena Laboratories Corporation Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
PH23572A (en) * 1982-05-28 1989-09-11 Smithkline Diagnostics Inc Specimen test slide for occult blood testing
US4486536A (en) * 1982-05-28 1984-12-04 Smithkline Diagnostics, Inc. Specimen slide for occult blood testing
US4493892A (en) * 1982-12-20 1985-01-15 Memorial Hospital For Cancer & Allied Diseases Solvent system for peroxidase inhibitor reagent for fecal occult blood determinations
CA1216215A (en) * 1983-03-01 1987-01-06 Henry J. Wells Peroxidase activity detection composition
US5702913A (en) * 1983-12-21 1997-12-30 Helena Laboratories Corporation Chromgen-reagent test system
US5273888A (en) * 1984-01-16 1993-12-28 Helena Laboratories Corporation Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
NO157437C (no) * 1985-08-30 1988-03-16 Scandinavian Analytical Produc Proeveinnretning for bestemmelse av blod i faeces.
EP0281251A3 (en) * 1987-02-04 1988-09-28 International Immunoassay Laboratories, Inc. A method for preparing a fecal sample composition for immunoassay testing
US5198365A (en) * 1987-02-04 1993-03-30 International Immunoassay Laboratories, Inc. Fecal sample immunoassay method testing for hemoglobin
JPH0684970B2 (ja) * 1987-03-31 1994-10-26 株式会社京都医科学研究所 糞便中の潜血検出方法
US5081040A (en) * 1987-06-29 1992-01-14 Helena Laboratories Corporation Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes
US5182191A (en) * 1988-10-14 1993-01-26 Pacific Biotech, Inc. Occult blood sampling device and assay
US5196167A (en) * 1989-04-04 1993-03-23 Helena Laboratories Corporation Fecal occult blood test product with positive and negative controls
US5217874A (en) * 1989-04-04 1993-06-08 Helena Laboratories Corporation Fecal occult blood test product with positive and negative controls
US20060154276A1 (en) 2004-05-13 2006-07-13 Prometheus Laboratories Inc. Methods of diagnosing inflammatory bowel disease
EP2359137A4 (en) 2008-11-11 2012-05-30 Prometheus Lab Inc METHOD FOR PREDICTING INFLAMMATORY ENDURANCE (IBD) DUE TO SEROLOGICAL MARKERS
EP2419529B1 (en) 2009-04-14 2015-05-20 Nestec S.A. Inflammatory bowel disease prognostics
CA2766157A1 (en) 2009-06-25 2010-12-29 Hua Gong Methods for diagnosing irritable bowel syndrome
WO2011060098A1 (en) 2009-11-10 2011-05-19 Prometheus Laboratories Inc. Methods for predicting post-surgery risk associated with ileal pouch-anal anastomosis
WO2013059732A1 (en) 2011-10-21 2013-04-25 Nestec S.A. Methods for improving inflammatory bowel disease diagnosis
US9091682B1 (en) 2014-05-01 2015-07-28 Steven M Hacker Tissue specimen bottle with color indicator in lid verifying and confirming presence of human tissue or blood contained in specimen bottle
EP3423830A4 (en) 2015-11-06 2020-04-15 Vetica Labs, Inc. METHOD FOR DETECTING INFLAMMATORY MARKERS AND TREATING INFLAMMATORY DISEASES IN PETS
CN110940639B (zh) * 2018-12-24 2023-03-07 河北省动物疫病预防控制中心 一种死宰羊肉的鉴定方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3350174A (en) * 1964-01-08 1967-10-31 Miles Lab Process of determining hemoglobin separated from peroxidase inhibitors in urine
US3919044A (en) * 1973-03-28 1975-11-11 Armour Pharma Processes for concentrating and purifying viruses and viral antigens
CA1056746A (en) * 1974-09-06 1979-06-19 Chung J. Lai Biologically active membrane material
US4005984A (en) * 1975-05-16 1977-02-01 Reese Fell Alsop Diagnostic composition and method of using the same
JPS5263794A (en) * 1975-11-21 1977-05-26 Shionogi Seiyaku Kk Test piece for latent blood
US4200690A (en) * 1976-12-16 1980-04-29 Millipore Corporation Immunoassay with membrane immobilized antibody
US4142856A (en) * 1977-12-02 1979-03-06 Isolab, Incorporated Method to determine a diagnostic indicator of blood sugar condition, and, a liquid chromatographic microcolumn therefor
US4138214A (en) * 1977-12-19 1979-02-06 American Home Products Corporation Diagnostic test utilizing human chorionic gonadotropin
US4175923A (en) * 1978-06-26 1979-11-27 Friend William G Method and apparatus for occult blood testing in the home
DE2853300C3 (de) * 1978-12-09 1982-12-02 Röhm GmbH, 6100 Darmstadt Schnelldiagnostica zur diagnostischen Erfassung von okkultem Blut

Also Published As

Publication number Publication date
EP0030388A2 (en) 1981-06-17
ES497544A0 (es) 1981-09-16
US4277250A (en) 1981-07-07
JPS5693045A (en) 1981-07-28
BR8008073A (pt) 1981-06-30
EP0030388A3 (en) 1982-01-20
NO803479L (no) 1981-06-11

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19980504