GB2280754A - Electrochemical detection of DNA hybridisation - Google Patents

Electrochemical detection of DNA hybridisation

Info

Publication number
GB2280754A
GB2280754A GB9419245A GB9419245A GB2280754A GB 2280754 A GB2280754 A GB 2280754A GB 9419245 A GB9419245 A GB 9419245A GB 9419245 A GB9419245 A GB 9419245A GB 2280754 A GB2280754 A GB 2280754A
Authority
GB
United Kingdom
Prior art keywords
hybridisation
signal
dna
dna hybridisation
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9419245A
Other versions
GB2280754B (en
GB9419245D0 (en
Inventor
Geoffrey Frank Hall
Jennifer Maeve Hall
Irving John Higgins
Joseph Victor Bannister
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Environmental and Medical Products Ltd
Original Assignee
Environmental and Medical Products Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Environmental and Medical Products Ltd filed Critical Environmental and Medical Products Ltd
Publication of GB9419245D0 publication Critical patent/GB9419245D0/en
Publication of GB2280754A publication Critical patent/GB2280754A/en
Application granted granted Critical
Publication of GB2280754B publication Critical patent/GB2280754B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Abstract

This invention relates to a method and apparatus for detecting DNA hybridisation and may be suitable for the detection of genetic defects. The invention provides a method of DNA hybridisation detection without the use of radioactive isotopes and includes the steps of: denaturing a specimen of DNA; contacting the specimen with an electrochemical electrode; measuring a reference polarographic signal characteristic of ssDNA; contacting the sample with an oligonucleotide probe under conditions where hybridisation may occur; removing any excess reagents; obtaining a polarographic signal from the electrode and comparing the signal with the reference signal. <IMAGE>
GB9419245A 1992-03-27 1993-03-26 Electrochemical detection of DNA hybridisation Expired - Fee Related GB2280754B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929206671A GB9206671D0 (en) 1992-03-27 1992-03-27 Electrochemical detection of dna hybridisation
PCT/GB1993/000631 WO1993020230A1 (en) 1992-03-27 1993-03-26 Electrochemical detection of dna hybridisation

Publications (3)

Publication Number Publication Date
GB9419245D0 GB9419245D0 (en) 1994-11-16
GB2280754A true GB2280754A (en) 1995-02-08
GB2280754B GB2280754B (en) 1995-11-01

Family

ID=10712937

Family Applications (2)

Application Number Title Priority Date Filing Date
GB929206671A Pending GB9206671D0 (en) 1992-03-27 1992-03-27 Electrochemical detection of dna hybridisation
GB9419245A Expired - Fee Related GB2280754B (en) 1992-03-27 1993-03-26 Electrochemical detection of DNA hybridisation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB929206671A Pending GB9206671D0 (en) 1992-03-27 1992-03-27 Electrochemical detection of dna hybridisation

Country Status (2)

Country Link
GB (2) GB9206671D0 (en)
WO (1) WO1993020230A1 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5871918A (en) * 1996-06-20 1999-02-16 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
US6180346B1 (en) 1995-06-27 2001-01-30 The Universtiy Of North Carolina At Chapel Hill Electropolymerizable film, and method of making and use thereof
US6132971A (en) * 1995-06-27 2000-10-17 The University Of North Carolina At Chapel Hill Microelectronic device
US6387625B1 (en) 1995-06-27 2002-05-14 The University Of North Carolina At Chapel Hill Monolayer and electrode for detecting a label-bearing target and method of use thereof
US6346387B1 (en) 1995-06-27 2002-02-12 Xanthon, Inc. Detection of binding reactions using labels detected by mediated catalytic electrochemistry
CN1192249A (en) * 1995-06-27 1998-09-02 查珀尔希尔北卡罗来纳大学 Electrochemical detection of nucleic acid hybridization
US6361951B1 (en) 1995-06-27 2002-03-26 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
US6127127A (en) * 1995-06-27 2000-10-03 The University Of North Carolina At Chapel Hill Monolayer and electrode for detecting a label-bearing target and method of use thereof
US5968745A (en) * 1995-06-27 1999-10-19 The University Of North Carolina At Chapel Hill Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof
US6689265B2 (en) 1995-10-11 2004-02-10 Therasense, Inc. Electrochemical analyte sensors using thermostable soybean peroxidase
US5665222A (en) * 1995-10-11 1997-09-09 E. Heller & Company Soybean peroxidase electrochemical sensor
EP1055004A2 (en) * 1998-02-20 2000-11-29 Wolfbeis, Otto Samuel Device for detecting oligonucleotide and/or polynucleotide hybridization
EP2273270A1 (en) 1998-08-28 2011-01-12 febit holding GmbH Method and apparatus for the preparation and/or analysis of biochemical carriers
DE19940647C2 (en) * 1999-08-26 2002-11-28 November Ag Molekulare Medizin Method for the detection and quantification of first biopolymers in a liquid
FR2805545B1 (en) * 2000-02-24 2002-05-17 Argene Sa ELECTROCHEMICAL METHOD FOR DETECTION OF NUCLEIC ACIDS
DE10051396A1 (en) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh An integrated synthesis and identification of an analyte, comprises particles immobilized at a carrier to be coupled to receptors in a structured pattern to give receptor arrays for biochemical reactions
US7202028B2 (en) 2001-09-24 2007-04-10 The University Of North Carolina At Chapel Hill Methods for the electrochemical detection of multiple target compounds
WO2004023128A1 (en) * 2002-09-05 2004-03-18 Unisearch Limited Detection of target nucleic acid molecules by alteration of reaction of a redox species following hybridization with immoblized capture nucleic acid molecules
WO2007006179A1 (en) * 2005-07-14 2007-01-18 Blincofa Biomedical Technology Corp. A graphite electrode used in detection device for analyzing trace amount of organic substances and its manufacturing method
ES2310125B1 (en) 2007-05-25 2010-01-07 Universidad Autonoma De Madrid METHOD FOR THE ELECTROCHEMICAL DETECTION OF NUCLEIC ACID SEQUENCES.
CN107988318B (en) * 2017-11-24 2021-05-25 杭州电子科技大学 Method for rapidly detecting nucleic acid based on electrochemical potential pretreatment technology and application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0149339A2 (en) * 1983-12-16 1985-07-24 MediSense, Inc. Assay for nucleic acids
DE3513168A1 (en) * 1985-04-12 1986-10-16 Thomas 8000 München Dandekar BIOSENSOR CONSISTING OF A SEMICONDUCTOR BASED ON SILICON OR CARBON-BASED (ELECTRONIC PART) AND NUCLEIN BASE (OR. OTHER BIOL. MONOMERS)
WO1987002066A1 (en) * 1985-09-26 1987-04-09 The University Of Southern Mississippi Piezoelectric device for detection of polynucleotide hybridization

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0149339A2 (en) * 1983-12-16 1985-07-24 MediSense, Inc. Assay for nucleic acids
DE3513168A1 (en) * 1985-04-12 1986-10-16 Thomas 8000 München Dandekar BIOSENSOR CONSISTING OF A SEMICONDUCTOR BASED ON SILICON OR CARBON-BASED (ELECTRONIC PART) AND NUCLEIN BASE (OR. OTHER BIOL. MONOMERS)
WO1987002066A1 (en) * 1985-09-26 1987-04-09 The University Of Southern Mississippi Piezoelectric device for detection of polynucleotide hybridization

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
& BULL CHEM SOC JPN 1978, 51(4), 1235-6 PY. *
BIOELECTROCHEM. BIOENERG. 1981, 8(4), 437-49. *
CHEMICAL ABSTRACTS, Vol 89, No. 1, 1987, page 174, No. 1823v *
CHEMICAL ABSTRACTS, Vol 94, No 23, 1981, page 271, No.187973a & STUD. BIOPHYS. 1981, 82(1), 17-26 PY *

Also Published As

Publication number Publication date
WO1993020230A1 (en) 1993-10-14
GB2280754B (en) 1995-11-01
GB9206671D0 (en) 1992-05-13
GB9419245D0 (en) 1994-11-16

Similar Documents

Publication Publication Date Title
GB2280754A (en) Electrochemical detection of DNA hybridisation
DE69425158D1 (en) OPTICAL SAMPLE ANALYSIS SYSTEM AND METHOD
NZ306051A (en) Testing using electrochemiluminescence
ATE172030T1 (en) METHOD AND DETECTOR FOR SEPARATION PROCESSES
TW368400B (en) Sample collection apparatus and kit with the same and detecting method using the same
MY107650A (en) Method of estimating property and / or composition data of a test sample
ZA978630B (en) Method for collecting samples of liquid specimens for analytical testing.
BG104275A (en) Method and device for express analysis of tested parameters in biological samples
DE69532953T8 (en) MOLECULAR ANALYZER AND METHOD FOR ITS USE
DE69711177D1 (en) SYSTEM FOR DETERMINING AT LEAST ONE PARAMETER AT LEAST ONE SAMPLE OF A PHYSIOLOGICAL LIQUID, AND CASSETTE THEREFOR
ZA965271B (en) Flow cell sensor apparatus and optical detection device for analytical measurements on flowable samples and methods of carrying out analytical and optical analytical measurements on flowable samples
DE3377362D1 (en) Method and apparatus for microbial identification (1111111)
DE59610100D1 (en) DEVICE FOR EXAMINING LIQUID SAMPLES
EP1211325A3 (en) Apparatus and cartridge for detecting biopolymers
ATE153764T1 (en) APPARATUS AND METHOD FOR PERFORMING BIOLOGICAL ASSAYS CONTAINING A PARTICLE SIEVING SYSTEM
US6251245B1 (en) Detecting and analyzing apparatus for positive ions and negative ions in a liquid
EP1455177A3 (en) A sample testing device and method
WO1998052012A3 (en) Measuring device and method for cleaning contaminated areas of a measuring device
GB2127977A (en) Method and sensor for the voltametric determination of oxygen
ATE464554T1 (en) METHOD AND DEVICE FOR TREATING SAMPLES
SUGIMOTO et al. Development of Automatic Inspection System Using a High Resolution Image Processor for Inclusion of Steel Products
Stottmeister et al. Some Aspects of the Isolation and Concentration of Volatile Organic Micropollutants From Water.
WEISCHEDEL Electromagnetic inspection of wire ropes using sensor arrays(Final Report, 16 Jun. 1983- 15 Jun. 1985)
ITRM980754A0 (en) METHOD FOR THE MEASUREMENT OF AN ANALYTE IN NON-ISOTHERMAL CONDITIONS AND BIOSENSOR APPARATUS FOR ITS EXECUTION

Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19970326