ES546597A0 - Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces. - Google Patents

Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces.

Info

Publication number
ES546597A0
ES546597A0 ES546597A ES546597A ES546597A0 ES 546597 A0 ES546597 A0 ES 546597A0 ES 546597 A ES546597 A ES 546597A ES 546597 A ES546597 A ES 546597A ES 546597 A0 ES546597 A0 ES 546597A0
Authority
ES
Spain
Prior art keywords
transformed
hospedant
sacharomyces
genre
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES546597A
Other languages
English (en)
Other versions
ES8802251A1 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24591220&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES546597(A0) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of ES546597A0 publication Critical patent/ES546597A0/es
Publication of ES8802251A1 publication Critical patent/ES8802251A1/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/68Stabilisation of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01006Galactokinase (2.7.1.6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0101Oligo-1,6-glucosidase (3.2.1.10), i.e. sucrase
ES546597A 1984-08-31 1985-08-30 Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces. Expired ES8802251A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US64597384A 1984-08-31 1984-08-31

Publications (2)

Publication Number Publication Date
ES546597A0 true ES546597A0 (es) 1988-04-16
ES8802251A1 ES8802251A1 (es) 1988-04-16

Family

ID=24591220

Family Applications (2)

Application Number Title Priority Date Filing Date
ES546597A Expired ES8802251A1 (es) 1984-08-31 1985-08-30 Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces.
ES554327A Expired ES8706825A1 (es) 1984-08-31 1986-04-24 Un procedimiento para preparar un plasmido recombinante

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES554327A Expired ES8706825A1 (es) 1984-08-31 1986-04-24 Un procedimiento para preparar un plasmido recombinante

Country Status (13)

Country Link
EP (1) EP0173668B2 (es)
JP (1) JPS6170981A (es)
KR (1) KR870002261A (es)
AT (1) ATE43637T1 (es)
AU (1) AU599574B2 (es)
CA (1) CA1263617A (es)
DE (1) DE3570694D1 (es)
DK (1) DK391185A (es)
ES (2) ES8802251A1 (es)
GR (1) GR852105B (es)
IL (1) IL76190A0 (es)
PT (1) PT81052B (es)
ZA (1) ZA856645B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ216353A (en) * 1985-06-05 1988-05-30 Univ Kentucky Res Found Manufacture of lac + fungi
EP0257115A1 (en) * 1986-08-21 1988-03-02 Heineken Technisch Beheer B.V. Amylolytic enzyme producing microorganisms, constructed by recombinant DNA technology, and their use in fermentation processes
US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
EP0260404B1 (en) * 1986-08-21 1995-05-31 VAN DEN BERG, Robert Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes
AU4005289A (en) * 1988-08-25 1990-03-01 Smithkline Beecham Corporation Recombinant saccharomyces
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0105149B1 (en) * 1982-08-16 1990-05-16 Science and Technology Agency, Minister's Secretariat, Director of Finance Division Recombinant plasmid containing hepatitis b virus gene, yeast transformed with said recombinant plasmid, and production of hepatitis b virus surface antigen
WO1984004538A1 (en) * 1983-05-19 1984-11-22 Unilever Nv Improvements in the expression of newly introduced genes in yeast cells

Also Published As

Publication number Publication date
EP0173668A1 (en) 1986-03-05
CA1263617A (en) 1989-12-05
ATE43637T1 (de) 1989-06-15
KR870002261A (ko) 1987-03-30
AU599574B2 (en) 1990-07-26
DK391185D0 (da) 1985-08-28
DE3570694D1 (en) 1989-07-06
ES554327A0 (es) 1987-07-01
JPS6170981A (ja) 1986-04-11
ES8706825A1 (es) 1987-07-01
GR852105B (es) 1985-12-24
PT81052B (pt) 1988-01-22
AU4682685A (en) 1986-03-06
PT81052A (en) 1985-09-01
EP0173668B2 (en) 1992-07-15
IL76190A0 (en) 1985-12-31
ZA856645B (en) 1986-07-30
ES8802251A1 (es) 1988-04-16
EP0173668B1 (en) 1989-05-31
DK391185A (da) 1986-03-01

Similar Documents

Publication Publication Date Title
ES2061433T3 (es) Metodo para la expresion a alto nivel de polipeptidos en levadura del genero pichia.
EP0185444A3 (en) Cloning and expression of htlv-iii dna
DE3586333D1 (de) Expressionsvektoren fuer hirudin, transformierte zellen und verfahren zur herstellung von hirudin.
DK496485D0 (da) Transformation af gaer-stammer af slaegten pichia
ATE54167T1 (de) Promotoren fuer die expression von fremden genen in hefe, plasmide, die diese promotoren enthalten, sowie deren verwendung zur herstellung von polypeptiden.
GB2007676A (en) Method and means for microbial polypeptide expression
DK0778348T3 (da) Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr
EP0362179A3 (en) Recombinant saccharomyces
ES546597A0 (es) Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces.
SE8101665L (sv) Polypeptidframstellning
ES2061524T3 (es) Un vector plasmidico para la expresion en bacillus y empleado para la clonacion del gen estructural que codifica la hormona de crecimiento humana y un metodo para la produccion de la hormona.
MX9308031A (es) Procedimiento para la produccion de polipeptidos.
ATE336585T1 (de) Modifikation von kryptischen splicemotiven heterologer gene zur expression in pilzen
KR890005262A (ko) 효모와 대장균의 두 가지 프로모터 활성을 가진 신규 dna(yep1) 및 그 분리방법

Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960506