KR870002261A - 복합효모균 - Google Patents

복합효모균 Download PDF

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KR870002261A
KR870002261A KR1019850006285A KR850006285A KR870002261A KR 870002261 A KR870002261 A KR 870002261A KR 1019850006285 A KR1019850006285 A KR 1019850006285A KR 850006285 A KR850006285 A KR 850006285A KR 870002261 A KR870002261 A KR 870002261A
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candida
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dna sequence
functional dna
yeast
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앤젤 고맨 제시카
콜틴 이갈
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리챠드 도미니크 포기오
스미스 클라인 베크만 코퍼레이션
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    • C12Y302/0101—Oligo-1,6-glucosidase (3.2.1.10), i.e. sucrase

Abstract

내용 없음

Description

복합효모균
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (30)

  1. 칸디다군으로 부터 얻은 기능 DNA 축차를 사용하여 효모균이 숙주미생물을 전형시키는 과정을 포함하여 동 전형된 숙주 미생물을 생성하는 방법.
  2. 기능 DNA 축차를 칸디다 백체, 칸디다 트로피칼리스, 칸디다 스텔라토이디아, 칸디다 프슈도트로피 칼리스, 칸디다 파라프실로시스 또는 칸디다 킬리에르몬디, 칸디다 유틸리스 또는 칸디다 크루이시등으로 부터 얻은 청구범위 1의 방법.
  3. 칸디다 백체로 부터 기능 DNA 축차를 얻는 청구범위 2의 방법.
  4. 기능 DNA 축차가 완전 유전자 표상 단위, 구조유전자, 촉진제 또는 복제부를 포함하는 청구범위 1의 방법.
  5. trpl 유전자, his3 유전자, 항-진균제 비노밀에 대한 저항을 부여하는 유전자, 탄소 소스 소르비톨상에서의 증식을 가능케하는 기능 DNA 축차, 용해성 전분 상에서의 증식을 가능케 하는 기능 DNA 축차,이소말토 제상에서의 증식을 가능케하는 기능 DNA 축차 또는 갈락토키나제 유전자로 부터 기능 DNA 축차를 얻는 청구범위 4의 방법.
  6. 세레비지어 효모균종에 속하는 청구범위 1의 방법
  7. 복합 플래시미드를 함유하는 경우로서, 동 플래스미드가 복제부 및 칸디다균으로부터 얻은 기능 DNA 축차를 포함하는 청구범위 1의 방법.
  8. 복제부가 효모균 또는 칸디다균으로부터 얻어지는 청구범위 7의 방법
  9. 복합 플래스미드가 효모균-대장균 이동매질인 경우의 청구범위 7의 방법
  10. 이동매질이 YEP13유도체인 청구범위 9의 방법
  11. 복제부 및 칸디다균으로부터 얻은 기능 DNA 축차를 함유하고 있으며, 동 복제부를 직접 또는 간접적으로 기능 DNA 축차와 결찰시키는 것을 포함하는 복합 플래스미드의 생성방법.
  12. 복제부가 효모균 또는 칸디다균으로 부터 얻어지는 청구범위 11의 방법.
  13. 기능 DNA 축차가 칸디다 백체, 칸디다 트로피칼리스, 칸디다 스텔라토 이디아, 칸디다 프슈도트로 피칼리스, 칸디다 파라프실로시스, 킨디다 킬리에르몬디, 칸디다 유틸리스 또는 킨다다 크루이시 등으로 부터 얻어지는 청구범위 11의 방법
  14. 기능 DNA 축차가 칸디다 백체로 부터 얻어지는 청구범위 13의 방법.
  15. 기능 DNA 축차가 완전 유전자 표상 단위, 구조유전자, 조촉매 또는 복제부를 포함하는 청구범위 11의 방법.
  16. 기능 DNA 축차가 trp1 유전자, his3 유전자, 항-진균제 비노밀에 대한 저항을 부여하는 유전자, 탄소 소스 소르비톨에서의 증식을 가능케 하는 유전자, 글루코말라제 유전자, 이소말타제 유전자 또는 갈락토키나제 유전자등으로 부터 얻어지는 청구범위 15의 방법
  17. 효모균-대장균 이동 매질에 해당하는 청구범위 11의 방법.
  18. 이동매질이 YEP13 유도체인 청구범위 17의 방법.
  19. 기능 DNA 축차로써 효모균의 숙주미생물을 전형시키는 것, 기능 DNA 축차가 표상될 수 있는 적당한 조건에서 전형된 숙주를 배양하는 것을 포함하여 칸디다균으로 부터 얻은 기능 DNA 축차를 표상하는 방법.
  20. 청구범위 19의 방법에 따라 칸디다 백체, 트로피칼리스, 칸디다 스텔라토 이디아, 칸디다 프슈도트로 피칼리스, 칸디다 파라프실로시스, 킨디다 킬리에르몬디, 칸디다 유틸리스 또는 킨다다 크르이시 등으로 부터 기능 DNA 축차를 얻는 방법.
  21. 청구범위 20에 따라 기능 DNA 축차를 칸디다 백체로 부터 얻는 방법.
  22. 기능 DNA 축차가 완전 유전자 표상단위, 구조유전자, 촉진제 또는 복제부를 함유하는 경우의 청구범위 19에 따른 방법.
  23. 기능 DNA 축차가 trp1 유전자, his3 유전자, 항-진균제 비노밀에 대한 저항을 부여하는 유전자, 탄소 소스 소르비톨에서의 증식을 가능케 하는 유전자, 글루코아말라제 유전자, 이소말타제 유전자 또는 갈락토키나제 유전자등으로 부터 얻어지는 경우의 청구범위 22에 따른 방법.
  24. 숙주 미생물이 세레비지어 효모균에 속하는 경우의 청구범위 19에 따른 방법.
  25. 복제부가 들어있는 복합 플래시미드 및 칸디다균으로부터 얻은 기능 DNA 축차로써 전형을 일으키는 것을 포함하는 청구범위 19에 따른 방법.
  26. 복제부가 효모균 또는 칸디다균으로 부터 얻어지는 청구범위 25에 따른 방법.
  27. 복합 플래스미드가 대장균 이동매질인 경우의 청구범위 25에 따른 방법.
  28. 이동매질이 YEP13 유도체인 경우의 청구범위 27에 따른 방법.
  29. 그 기능을 통하여 칸디다균으로 얻어지는 DNA 축차를 특성화시키는 방법으로써, 가) 숙주미생물이 공지의 기능을 결여하고 있어서 DNA 축차로써 효모균의 숙주미생물을 전형시켜 칸디다균 DNA가 숙주미생물의 DNA를 있도록 하고 나) 전형된 숙주미생물에서 기능의 존재를 분석하는 방법.
  30. 직접 또는 간접으로 폴리펩티드를 조성하는 구조유전자에 위상적으로 및 적당한 배열로 흡착되어 있는 칸디다균으로 부터 얻은 촉진제가 들어있는 효모균의 전형된 숙주미생물의 배양을 포함하여, 효모균내의 폴리펩티드를 생성하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019850006285A 1984-08-31 1985-08-30 복합효모균 KR870002261A (ko)

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JP (1) JPS6170981A (ko)
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AT (1) ATE43637T1 (ko)
AU (1) AU599574B2 (ko)
CA (1) CA1263617A (ko)
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NZ216353A (en) * 1985-06-05 1988-05-30 Univ Kentucky Res Found Manufacture of lac + fungi
EP0257115A1 (en) * 1986-08-21 1988-03-02 Heineken Technisch Beheer B.V. Amylolytic enzyme producing microorganisms, constructed by recombinant DNA technology, and their use in fermentation processes
US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
EP0260404B1 (en) * 1986-08-21 1995-05-31 VAN DEN BERG, Robert Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes
AU4005289A (en) * 1988-08-25 1990-03-01 Smithkline Beecham Corporation Recombinant saccharomyces
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system

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EP0105149B1 (en) * 1982-08-16 1990-05-16 Science and Technology Agency, Minister's Secretariat, Director of Finance Division Recombinant plasmid containing hepatitis b virus gene, yeast transformed with said recombinant plasmid, and production of hepatitis b virus surface antigen
WO1984004538A1 (en) * 1983-05-19 1984-11-22 Unilever Nv Improvements in the expression of newly introduced genes in yeast cells

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EP0173668A1 (en) 1986-03-05
CA1263617A (en) 1989-12-05
ATE43637T1 (de) 1989-06-15
AU599574B2 (en) 1990-07-26
DK391185D0 (da) 1985-08-28
DE3570694D1 (en) 1989-07-06
ES554327A0 (es) 1987-07-01
JPS6170981A (ja) 1986-04-11
ES8706825A1 (es) 1987-07-01
GR852105B (ko) 1985-12-24
PT81052B (pt) 1988-01-22
AU4682685A (en) 1986-03-06
ES546597A0 (es) 1988-04-16
PT81052A (en) 1985-09-01
EP0173668B2 (en) 1992-07-15
IL76190A0 (en) 1985-12-31
ZA856645B (en) 1986-07-30
ES8802251A1 (es) 1988-04-16
EP0173668B1 (en) 1989-05-31
DK391185A (da) 1986-03-01

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