ES338445A1 - Manufacture of Purified Amyloglucosidase Preparations. - Google Patents

Manufacture of Purified Amyloglucosidase Preparations.

Info

Publication number
ES338445A1
ES338445A1 ES338445A ES338445A ES338445A1 ES 338445 A1 ES338445 A1 ES 338445A1 ES 338445 A ES338445 A ES 338445A ES 338445 A ES338445 A ES 338445A ES 338445 A1 ES338445 A1 ES 338445A1
Authority
ES
Spain
Prior art keywords
manufacture
solution
transglucosidase
amyloglucosidase
aqueous solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES338445A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Organon NV
Original Assignee
Organon NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Organon NV filed Critical Organon NV
Publication of ES338445A1 publication Critical patent/ES338445A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A process for the purification of amyloglucosidase preparations comprises the inactivation in situ, without removal, of transglucosidase contained in an aqueous solution of the impure enzyme by incubating the solution at a pH below 2À5 without adding auxiliary substances other than a pH regulating compound which does not cause precipitation of the transglucosidase and which does not have surface-active properties. The compound added may be hydrochloric, sulphuric, phosphoric, citric, oxalic, acetic or formic acid. The aqueous solution treated may comprise the entire fermentation broth in which the amyloglucosidase has been formed, a culture filtrate, or a solution of an enzyme concentrate.
ES338445A 1966-03-26 1967-03-25 Manufacture of Purified Amyloglucosidase Preparations. Expired ES338445A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL6604012A NL6604012A (en) 1966-03-26 1966-03-26

Publications (1)

Publication Number Publication Date
ES338445A1 true ES338445A1 (en) 1968-04-01

Family

ID=19796102

Family Applications (1)

Application Number Title Priority Date Filing Date
ES338445A Expired ES338445A1 (en) 1966-03-26 1967-03-25 Manufacture of Purified Amyloglucosidase Preparations.

Country Status (6)

Country Link
BE (1) BE696051A (en)
DK (1) DK113704B (en)
ES (1) ES338445A1 (en)
FR (1) FR1515697A (en)
GB (1) GB1180802A (en)
NL (1) NL6604012A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4532213A (en) * 1983-12-08 1985-07-30 Miles Laboratories, Inc. Recovery of acid fungal protease

Also Published As

Publication number Publication date
DK113704B (en) 1969-04-21
FR1515697A (en) 1968-03-01
NL6604012A (en) 1967-09-27
BE696051A (en) 1967-09-25
GB1180802A (en) 1970-02-11

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