GB1288843A - - Google Patents
Info
- Publication number
- GB1288843A GB1288843A GB1288843DA GB1288843A GB 1288843 A GB1288843 A GB 1288843A GB 1288843D A GB1288843D A GB 1288843DA GB 1288843 A GB1288843 A GB 1288843A
- Authority
- GB
- United Kingdom
- Prior art keywords
- broth
- benzylpenicillin
- lysozyme
- added
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/84—Penicillin amidase (3.5.1.11)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
1288843 Enzymes PFIZER CORP 15 April 1970 [12 July 1969] 18070/70 Heading C3H [Also in Divisions C2 and C6] The isolation yield of fermentation product, such as antibiotic or penicillin acylase, from a fermentation broth is increased by introducing protease, cellulase or lysozyme and, optionally, a chelating agent e.g. ethylenediamine tetraacetic acid or tris(hydroxy-ethyl) amino-methane, into the broth before isolating the product. The following enzymes are mentioned: proteases including bromelain, chymotrypsin, ficin, papain, pepsin and trypsin; cellulases; and various lysozymes. The enzyme and chelating agent should each be added in concentrations of at least 10 p.p.m. According to one of the examples, lysozyme is added at a level of 100 p.p.m. to a whole culture fermentation broth of Proteus rettgeri ATCC 9918. Quantitative penicillin acylase present is determined by the addition of benzylpenicillin which is hydrolysed to 6-amino penicillanic acid. The broth is filtered, adjusted to pH 2À5-3À0 with dilute hydrochloric acid and extracted with methyl isobutyl ketone to remove unconverted benzylpenicillin. The pH of the aqueous 6 - aminopenicillanic acid - containing phase is raised to pH 7À5 and phenylacetyl chloride is added. After 50 minutes at room temperature with continuous agitation, the pH is adjusted to 6À5 and the broth is bioassayed for benzylpenicillin. Significantly higher levels of penicillin acylase were obtained with the lysozyme treated broth.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5495569 | 1969-07-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1288843A true GB1288843A (en) | 1972-09-13 |
Family
ID=12985075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1288843D Expired GB1288843A (en) | 1969-07-12 | 1970-04-15 |
Country Status (3)
| Country | Link |
|---|---|
| ES (1) | ES381708A1 (en) |
| FR (1) | FR2051711B1 (en) |
| GB (1) | GB1288843A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10329819A1 (en) * | 2003-06-25 | 2005-01-20 | Technische Universität Dresden | Isolating organic compounds from cell cultures, useful for recovering chiral intermediates for pharmaceuticals, includes organic extraction, after incubation with hydrolase to destroy bioemulsifiers |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1548078A (en) * | 1977-03-14 | 1979-07-04 | Tate & Lyle Ltd | Process for the production of polysaccarides of controlled viscosity |
| US4119491A (en) * | 1977-05-16 | 1978-10-10 | Shell Oil Company | Enzyme-filtration clarification of xanthan gum polymer solution |
| EP0061250A3 (en) * | 1981-03-23 | 1983-09-28 | Biogen N.V. | Improved process for recovering products produced by host organisms |
-
1970
- 1970-04-15 GB GB1288843D patent/GB1288843A/en not_active Expired
- 1970-07-10 FR FR707025818A patent/FR2051711B1/fr not_active Expired
- 1970-07-11 ES ES381708A patent/ES381708A1/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10329819A1 (en) * | 2003-06-25 | 2005-01-20 | Technische Universität Dresden | Isolating organic compounds from cell cultures, useful for recovering chiral intermediates for pharmaceuticals, includes organic extraction, after incubation with hydrolase to destroy bioemulsifiers |
| DE10329819B4 (en) * | 2003-06-25 | 2006-11-02 | Technische Universität Dresden | Process for the extractive isolation of organic components from cell suspensions and cell-free media |
Also Published As
| Publication number | Publication date |
|---|---|
| ES381708A1 (en) | 1972-12-01 |
| FR2051711A1 (en) | 1971-04-09 |
| FR2051711B1 (en) | 1973-04-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| 48S | Specification amended (sect. 8/1949) | ||
| PCNP | Patent ceased through non-payment of renewal fee |