GB1288843A - - Google Patents

Info

Publication number
GB1288843A
GB1288843A GB1288843DA GB1288843A GB 1288843 A GB1288843 A GB 1288843A GB 1288843D A GB1288843D A GB 1288843DA GB 1288843 A GB1288843 A GB 1288843A
Authority
GB
United Kingdom
Prior art keywords
broth
benzylpenicillin
lysozyme
added
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of GB1288843A publication Critical patent/GB1288843A/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1288843 Enzymes PFIZER CORP 15 April 1970 [12 July 1969] 18070/70 Heading C3H [Also in Divisions C2 and C6] The isolation yield of fermentation product, such as antibiotic or penicillin acylase, from a fermentation broth is increased by introducing protease, cellulase or lysozyme and, optionally, a chelating agent e.g. ethylenediamine tetraacetic acid or tris(hydroxy-ethyl) amino-methane, into the broth before isolating the product. The following enzymes are mentioned: proteases including bromelain, chymotrypsin, ficin, papain, pepsin and trypsin; cellulases; and various lysozymes. The enzyme and chelating agent should each be added in concentrations of at least 10 p.p.m. According to one of the examples, lysozyme is added at a level of 100 p.p.m. to a whole culture fermentation broth of Proteus rettgeri ATCC 9918. Quantitative penicillin acylase present is determined by the addition of benzylpenicillin which is hydrolysed to 6-amino penicillanic acid. The broth is filtered, adjusted to pH 2À5-3À0 with dilute hydrochloric acid and extracted with methyl isobutyl ketone to remove unconverted benzylpenicillin. The pH of the aqueous 6 - aminopenicillanic acid - containing phase is raised to pH 7À5 and phenylacetyl chloride is added. After 50 minutes at room temperature with continuous agitation, the pH is adjusted to 6À5 and the broth is bioassayed for benzylpenicillin. Significantly higher levels of penicillin acylase were obtained with the lysozyme treated broth.
GB1288843D 1969-07-12 1970-04-15 Expired GB1288843A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5495569 1969-07-12

Publications (1)

Publication Number Publication Date
GB1288843A true GB1288843A (en) 1972-09-13

Family

ID=12985075

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1288843D Expired GB1288843A (en) 1969-07-12 1970-04-15

Country Status (3)

Country Link
ES (1) ES381708A1 (en)
FR (1) FR2051711B1 (en)
GB (1) GB1288843A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10329819A1 (en) * 2003-06-25 2005-01-20 Technische Universität Dresden Isolating organic compounds from cell cultures, useful for recovering chiral intermediates for pharmaceuticals, includes organic extraction, after incubation with hydrolase to destroy bioemulsifiers

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1548078A (en) * 1977-03-14 1979-07-04 Tate & Lyle Ltd Process for the production of polysaccarides of controlled viscosity
US4119491A (en) * 1977-05-16 1978-10-10 Shell Oil Company Enzyme-filtration clarification of xanthan gum polymer solution
EP0061250A3 (en) * 1981-03-23 1983-09-28 Biogen N.V. Improved process for recovering products produced by host organisms

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10329819A1 (en) * 2003-06-25 2005-01-20 Technische Universität Dresden Isolating organic compounds from cell cultures, useful for recovering chiral intermediates for pharmaceuticals, includes organic extraction, after incubation with hydrolase to destroy bioemulsifiers
DE10329819B4 (en) * 2003-06-25 2006-11-02 Technische Universität Dresden Process for the extractive isolation of organic components from cell suspensions and cell-free media

Also Published As

Publication number Publication date
ES381708A1 (en) 1972-12-01
FR2051711B1 (en) 1973-04-27
FR2051711A1 (en) 1971-04-09

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Legal Events

Date Code Title Description
PS Patent sealed
48S Specification amended (sect. 8/1949)
PCNP Patent ceased through non-payment of renewal fee