GB1184407A - Process for the Recovery of Glucose Oxidase - Google Patents

Process for the Recovery of Glucose Oxidase

Info

Publication number
GB1184407A
GB1184407A GB54669/68A GB5466968A GB1184407A GB 1184407 A GB1184407 A GB 1184407A GB 54669/68 A GB54669/68 A GB 54669/68A GB 5466968 A GB5466968 A GB 5466968A GB 1184407 A GB1184407 A GB 1184407A
Authority
GB
United Kingdom
Prior art keywords
glucose oxidase
nov
precipitate
recovery
vacuumconcentrated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB54669/68A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19671642592 external-priority patent/DE1642592C/en
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of GB1184407A publication Critical patent/GB1184407A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1,184,407. Glucose oxidase. BOEHRINGER MANNHEIM G.m.b.H. 18 Nov., 1968 [20 Nov., 1967], No.54669/68. Heading C3H. Pure glucose oxidase is recovered by adjusting the pH of an aqueous solution of impure glucose oxidase to 6À5-7À5 and while maintaining the temperature between 15‹ C. and 25‹ C. adding methanol to a concentration of 58-62% by volume and separating the precipitate. The glucose oxidase may be that produced by Penicillium notatum or Aspergillus niger. The precipitate may be mixed with a filter acid, filtered off, and extracted with water, and the aqueous extract vacuumconcentrated and lyophilized.
GB54669/68A 1967-11-20 1968-11-18 Process for the Recovery of Glucose Oxidase Expired GB1184407A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19671642592 DE1642592C (en) 1967-11-20 1967-11-20 Process for the production of glucose oxidase which is practically free of extraneous oxidase

Publications (1)

Publication Number Publication Date
GB1184407A true GB1184407A (en) 1970-03-18

Family

ID=5684196

Family Applications (1)

Application Number Title Priority Date Filing Date
GB54669/68A Expired GB1184407A (en) 1967-11-20 1968-11-18 Process for the Recovery of Glucose Oxidase

Country Status (3)

Country Link
US (1) US3645851A (en)
GB (1) GB1184407A (en)
SE (1) SE351852B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021127479A1 (en) * 2019-12-18 2021-06-24 Danisco Us Inc Stabilization of oxidases by drying under reduced partial oxygen pressure

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2308013A1 (en) * 1973-02-17 1974-09-12 Behringwerke Ag LOW CATALASE GLUCOSE OXIDASE AND METHOD FOR OBTAINING IT
US5270194A (en) * 1989-08-31 1993-12-14 Instrumentation Laboratory Spa Stabilized glucose oxidase from Aspergillus Niger

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2977287A (en) * 1957-11-25 1961-03-28 Ethicon Inc Enzyme and method for preparation
US2926122A (en) * 1958-02-24 1960-02-23 Dawe S Lab Inc Method of preparing glucose oxidase
US3136703A (en) * 1958-04-22 1964-06-09 Ortho Pharma Corp Method for obtaining fibrinolysin
NL259609A (en) * 1959-12-31
US3269918A (en) * 1964-04-20 1966-08-30 Miles Lab Process for the production of purified glucose oxidase
US3265587A (en) * 1964-05-25 1966-08-09 Miles Lab Process of purifying glucose oxidase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021127479A1 (en) * 2019-12-18 2021-06-24 Danisco Us Inc Stabilization of oxidases by drying under reduced partial oxygen pressure

Also Published As

Publication number Publication date
US3645851A (en) 1972-02-29
DE1642592A1 (en) 1971-05-06
SE351852B (en) 1972-12-11

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years