GB1156900A - Process for the Isolation of Enzymes - Google Patents
Process for the Isolation of EnzymesInfo
- Publication number
- GB1156900A GB1156900A GB2842268A GB2842268A GB1156900A GB 1156900 A GB1156900 A GB 1156900A GB 2842268 A GB2842268 A GB 2842268A GB 2842268 A GB2842268 A GB 2842268A GB 1156900 A GB1156900 A GB 1156900A
- Authority
- GB
- United Kingdom
- Prior art keywords
- protein
- enzyme
- enzymes
- tannin
- precipitation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1,156,900. Isolating enzymes. ARZNEIMITTELWERK DRESDEN VEB. 14 June, 1968, No. 28422/68. Heading C3H. In a process for the isolation of microbially produced enzymes by precipitation with tannin, a biological fermentation solution is mixed with such an amount of soluble precipitatable protein that in the culture filtrate a coprecipitation of the said protein and the enzyme is achieved or the precipitation of the enzyme is completed, the specific activity of the isolated enzyme concentrate being adjusted by alteration of the amount of protein added. The added protein may be casein, milk, dried milk, whey, peptone, gelatin, oxygelatin or maize steep liquor. The co-precipitate may be freed from tannin with acetone. Examples of isolating protease and amylase from Aspergillus and Streptomyces species are given.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2842268A GB1156900A (en) | 1968-06-14 | 1968-06-14 | Process for the Isolation of Enzymes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2842268A GB1156900A (en) | 1968-06-14 | 1968-06-14 | Process for the Isolation of Enzymes |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1156900A true GB1156900A (en) | 1969-07-02 |
Family
ID=10275386
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2842268A Expired GB1156900A (en) | 1968-06-14 | 1968-06-14 | Process for the Isolation of Enzymes |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1156900A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2193029A1 (en) * | 1972-07-13 | 1974-02-15 | Henkel & Cie Gmbh | Protease pptn - with lactalbumin or skim milk powder as stabilising agent |
FR2397422A1 (en) * | 1977-07-12 | 1979-02-09 | Tanabe Seiyaku Co | PRODUCT CONTAINING IMMOBILIZED AMINOACYLASE AND ITS PREPARATION |
ES2037603A1 (en) * | 1990-11-10 | 1993-06-16 | Roehm Gmbh | Preparations containing protease-tannin complex |
US6451586B1 (en) | 1990-11-10 | 2002-09-17 | Roehm Gmbh & Co Kg | Enzyme preparation containing protease |
CN109879845A (en) * | 2019-02-01 | 2019-06-14 | 广州柿宝生物科技有限公司 | Process for extracting flavone from persimmon calyx |
-
1968
- 1968-06-14 GB GB2842268A patent/GB1156900A/en not_active Expired
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2193029A1 (en) * | 1972-07-13 | 1974-02-15 | Henkel & Cie Gmbh | Protease pptn - with lactalbumin or skim milk powder as stabilising agent |
FR2397422A1 (en) * | 1977-07-12 | 1979-02-09 | Tanabe Seiyaku Co | PRODUCT CONTAINING IMMOBILIZED AMINOACYLASE AND ITS PREPARATION |
ES2037603A1 (en) * | 1990-11-10 | 1993-06-16 | Roehm Gmbh | Preparations containing protease-tannin complex |
US6451586B1 (en) | 1990-11-10 | 2002-09-17 | Roehm Gmbh & Co Kg | Enzyme preparation containing protease |
CN109879845A (en) * | 2019-02-01 | 2019-06-14 | 广州柿宝生物科技有限公司 | Process for extracting flavone from persimmon calyx |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |