ES2776157T3 - Citómetro de clasificación de flujo, método y medios legibles por computadora - Google Patents
Citómetro de clasificación de flujo, método y medios legibles por computadora Download PDFInfo
- Publication number
- ES2776157T3 ES2776157T3 ES13706356T ES13706356T ES2776157T3 ES 2776157 T3 ES2776157 T3 ES 2776157T3 ES 13706356 T ES13706356 T ES 13706356T ES 13706356 T ES13706356 T ES 13706356T ES 2776157 T3 ES2776157 T3 ES 2776157T3
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- Spain
- Prior art keywords
- drop
- drops
- fluid stream
- unwanted
- droplet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 49
- 239000012530 fluid Substances 0.000 claims abstract description 127
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims description 94
- 238000012545 processing Methods 0.000 claims description 14
- 230000007935 neutral effect Effects 0.000 claims description 5
- 230000015654 memory Effects 0.000 description 20
- 238000010586 diagram Methods 0.000 description 14
- 238000004891 communication Methods 0.000 description 9
- 230000006870 function Effects 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000005286 illumination Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 201000005569 Gout Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000007723 transport mechanism Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
- G01N15/1492—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties within droplets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1021—Measuring mass of individual particles
Landscapes
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261597022P | 2012-02-09 | 2012-02-09 | |
| PCT/US2013/025308 WO2013119924A1 (en) | 2012-02-09 | 2013-02-08 | Sorting flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2776157T3 true ES2776157T3 (es) | 2020-07-29 |
Family
ID=47750827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13706356T Active ES2776157T3 (es) | 2012-02-09 | 2013-02-08 | Citómetro de clasificación de flujo, método y medios legibles por computadora |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10190959B2 (h) |
| EP (1) | EP2812671B1 (h) |
| JP (1) | JP6192665B2 (h) |
| CN (1) | CN104204768B (h) |
| AU (1) | AU2013216878B2 (h) |
| ES (1) | ES2776157T3 (h) |
| IN (1) | IN2014DN06692A (h) |
| SG (1) | SG11201404780VA (h) |
| WO (1) | WO2013119924A1 (h) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012094325A2 (en) * | 2011-01-03 | 2012-07-12 | Cytonome/St. Llc | Method and apparatus for monitoring and optimizing particle sorting |
| EP2681567B1 (en) | 2011-03-03 | 2021-04-28 | Life Technologies Corporation | Sampling probes |
| US10309891B2 (en) * | 2013-10-16 | 2019-06-04 | Sony Corporation | Particle sorting apparatus, particle sorting method, and program |
| JP6136843B2 (ja) * | 2013-10-17 | 2017-05-31 | ソニー株式会社 | 粒子分取装置、粒子分取方法及びプログラム |
| US20150160246A1 (en) | 2013-12-11 | 2015-06-11 | Analiza, Inc. | Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells |
| US9709556B2 (en) | 2013-12-11 | 2017-07-18 | Analiza, Inc. | Devices and methods for determining and/or isolating circulating cancer cells |
| USD787701S1 (en) * | 2014-03-21 | 2017-05-23 | Intellicyt Corporation | Flow cytometer system shelf unit |
| JP2016080563A (ja) * | 2014-10-20 | 2016-05-16 | 日本光電工業株式会社 | 分析システム、分析装置 |
| US10091279B2 (en) | 2015-05-27 | 2018-10-02 | FlowJo, LLC | Wireless connected laboratory |
| JP6922901B2 (ja) * | 2016-05-17 | 2021-08-18 | ソニーグループ株式会社 | 粒子分取装置及び粒子分取方法 |
| CN109863384B (zh) | 2016-06-10 | 2022-10-28 | 加利福尼亚大学董事会 | 基于图像的细胞分选系统和方法 |
| US20190257736A1 (en) * | 2016-06-21 | 2019-08-22 | Synelixis AB | System and Method for Precision Deposition of Liquid Droplets |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| EP4215616B1 (en) | 2017-05-18 | 2024-09-04 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| CN112136033B (zh) * | 2018-04-27 | 2024-10-15 | 贝克顿·迪金森公司 | 具有气溶胶含量受控的封闭式液滴分选仪的流式细胞仪及其使用方法 |
| EP3921622A4 (en) | 2019-02-08 | 2022-11-09 | Becton, Dickinson and Company | DECISION MODULES FOR SORTING DROPLET, SYSTEMS AND METHODS OF USE THEREOF |
| CN109916803A (zh) * | 2019-02-27 | 2019-06-21 | 苏州朗如精密机械科技有限公司 | 一种流式细胞分析仪样本分选检测模块及其全自动分选检测方法 |
| EP3956649A4 (en) | 2019-04-19 | 2023-01-04 | Becton, Dickinson and Company | Subsampling flow cytometric event data |
| CN112730408B (zh) * | 2020-12-24 | 2024-07-09 | 贝克曼库尔特生物科技（苏州）有限公司 | 液流检测系统和液流检测方法以及分选装置 |
| CN115824736B (zh) * | 2022-11-10 | 2023-10-20 | 东西分析仪器(天津)有限公司 | 一种微量样品点样头 |
| CN115962995B (zh) * | 2022-12-26 | 2023-07-04 | 中国科学院基础医学与肿瘤研究所(筹) | 一种全自动微生物染色制片装置 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4347935A (en) * | 1979-05-16 | 1982-09-07 | The United States Of America As Represented By The United States Department Of Energy | Method and apparatus for electrostatically sorting biological cells |
| JPS62167478A (ja) * | 1985-11-29 | 1987-07-23 | Shimadzu Corp | 粒子分取装置 |
| US4981580A (en) * | 1989-05-01 | 1991-01-01 | Coulter Corporation | Coincidence arbitration in a flow cytomery sorting system |
| US5199576A (en) * | 1991-04-05 | 1993-04-06 | University Of Rochester | System for flexibly sorting particles |
| US5483469A (en) | 1993-08-02 | 1996-01-09 | The Regents Of The University Of California | Multiple sort flow cytometer |
| US5617911A (en) | 1995-09-08 | 1997-04-08 | Aeroquip Corporation | Method and apparatus for creating a free-form three-dimensional article using a layer-by-layer deposition of a support material and a deposition material |
| US6211477B1 (en) * | 1998-02-26 | 2001-04-03 | Becton Dickinson And Company | Electrostatic deceleration system for flow cytometer |
| US6281018B1 (en) | 1998-02-26 | 2001-08-28 | Coulter International Corp. | Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions |
| US6248590B1 (en) * | 1998-02-27 | 2001-06-19 | Cytomation, Inc. | Method and apparatus for flow cytometry |
| US6079836A (en) * | 1998-07-20 | 2000-06-27 | Coulter International Corp. | Flow cytometer droplet break-off location adjustment mechanism |
| US20030211009A1 (en) * | 2001-05-18 | 2003-11-13 | Buchanan Kris S. | Rapid multi-material sample input system |
| EP1468266A4 (en) * | 2002-01-22 | 2009-03-11 | Beckman Coulter Inc | ENVIRONMENTALLY COMPLETED SYSTEM FOR A FLOW CYTOMETER |
| US20110177547A1 (en) | 2004-12-10 | 2011-07-21 | Arryx, Inc. | Particle Sorting Using Fluid Streams |
| US8290625B2 (en) * | 2007-04-04 | 2012-10-16 | Beckman Coulter, Inc. | Flow cytometer sorter |
| US7691636B2 (en) | 2007-05-23 | 2010-04-06 | Beckman Coulter, Inc. | Method and apparatus for compensating for variations in particle trajectories in electrostatic sorter for flowcell cytometer |
| JP4990746B2 (ja) | 2007-12-14 | 2012-08-01 | ベイバイオサイエンス株式会社 | 液体フローに含まれる生物学的粒子を分別する装置ならびにその方法 |
| US8140300B2 (en) * | 2008-05-15 | 2012-03-20 | Becton, Dickinson And Company | High throughput flow cytometer operation with data quality assessment and control |
| JP5446563B2 (ja) | 2009-08-06 | 2014-03-19 | ソニー株式会社 | 微小粒子分取装置、および該微小粒子分取装置を用いたフローサイトメーター |
| JP2011237201A (ja) * | 2010-05-06 | 2011-11-24 | Sony Corp | 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール |
| US8705031B2 (en) * | 2011-02-04 | 2014-04-22 | Cytonome/St, Llc | Particle sorting apparatus and method |
| US9897530B2 (en) * | 2011-08-25 | 2018-02-20 | Sony Corporation | Compensation of motion-related error in a stream of moving micro-entities |
-
2013
- 2013-02-08 US US14/377,623 patent/US10190959B2/en active Active
- 2013-02-08 EP EP13706356.6A patent/EP2812671B1/en active Active
- 2013-02-08 ES ES13706356T patent/ES2776157T3/es active Active
- 2013-02-08 SG SG11201404780VA patent/SG11201404780VA/en unknown
- 2013-02-08 WO PCT/US2013/025308 patent/WO2013119924A1/en active Application Filing
- 2013-02-08 AU AU2013216878A patent/AU2013216878B2/en active Active
- 2013-02-08 CN CN201380018973.1A patent/CN104204768B/zh active Active
- 2013-02-08 IN IN6692DEN2014 patent/IN2014DN06692A/en unknown
- 2013-02-08 JP JP2014556717A patent/JP6192665B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2812671B1 (en) | 2019-12-25 |
| AU2013216878B2 (en) | 2016-09-29 |
| US20150050688A1 (en) | 2015-02-19 |
| CN104204768A (zh) | 2014-12-10 |
| AU2013216878A1 (en) | 2014-08-28 |
| WO2013119924A1 (en) | 2013-08-15 |
| JP2015507204A (ja) | 2015-03-05 |
| EP2812671A1 (en) | 2014-12-17 |
| US10190959B2 (en) | 2019-01-29 |
| IN2014DN06692A (h) | 2015-05-22 |
| CN104204768B (zh) | 2018-07-10 |
| JP6192665B2 (ja) | 2017-09-06 |
| SG11201404780VA (en) | 2014-09-26 |
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