ES2741956T3 - Aislamiento de ácidos nucleicos - Google Patents
Aislamiento de ácidos nucleicos Download PDFInfo
- Publication number
- ES2741956T3 ES2741956T3 ES14705753T ES14705753T ES2741956T3 ES 2741956 T3 ES2741956 T3 ES 2741956T3 ES 14705753 T ES14705753 T ES 14705753T ES 14705753 T ES14705753 T ES 14705753T ES 2741956 T3 ES2741956 T3 ES 2741956T3
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- Prior art keywords
- nucleic acid
- sample
- lysate
- composition
- liquefaction
- Prior art date
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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| CN111500680B (zh) | 2013-06-25 | 2024-04-16 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
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| EP3782731A1 (en) * | 2015-06-10 | 2021-02-24 | Biocartis N.V. | Improved detection of methylated dna |
| CN105861659A (zh) * | 2016-04-13 | 2016-08-17 | 武汉康录生物技术有限公司 | 一种用于荧光原位杂交ffpe组织切片的预处理液及预处理方法 |
| EP3586129B1 (en) | 2017-03-28 | 2023-07-12 | Phase Scientific International, Ltd. | Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy |
| SG11201909324WA (en) | 2017-06-01 | 2019-11-28 | Yin To Chiu | Phase separation behavior modifying agents for aqueous two-phase separation within porous material |
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| US20200318153A1 (en) * | 2017-09-01 | 2020-10-08 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for dental and oral diseases |
| US20200208137A1 (en) | 2017-09-18 | 2020-07-02 | Yin To Chiu | Method for Using Aqueous Two-Phase System for the Isolation, Purification and/or Concentration of Short Nucleic Acid Fragments |
| EP3740588A4 (en) | 2018-01-19 | 2021-10-20 | Phase Scientific International, Ltd. | METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM |
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| EP4752521A3 (en) | 2019-12-23 | 2026-08-12 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
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| EP4164796A4 (en) | 2020-06-10 | 2024-03-06 | 10x Genomics, Inc. | Fluid delivery methods |
| CN116194590A (zh) | 2020-09-18 | 2023-05-30 | 10X基因组学有限公司 | 样品处理设备和图像配准方法 |
| KR20230098259A (ko) | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
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| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
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| US5538870A (en) | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
| JP4503712B2 (ja) | 1996-09-09 | 2010-07-14 | 株式会社島津製作所 | 核酸合成法 |
| US6469159B1 (en) | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US20030134292A1 (en) | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| CN1922331A (zh) * | 2003-10-16 | 2007-02-28 | 第三次浪潮技术公司 | 体液的直接核酸检测 |
| CN1918291A (zh) * | 2003-12-24 | 2007-02-21 | 3M创新有限公司 | 使用微流体装置和沉降试剂分离核酸的方法和试剂盒 |
| JP5086250B2 (ja) | 2005-06-23 | 2012-11-28 | ビオカルティ ソシエテ アノニム | 自動医療診断用のカートリッジ、システム及び方法 |
| CN101213022B (zh) | 2005-06-30 | 2010-06-09 | 拜欧卡蒂斯股份公司 | 自动医学诊断盒体 |
| CA2680801A1 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation and/or stabilizationof a cell and/or macromolecule |
| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
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| EP2454378B1 (en) | 2009-07-17 | 2015-12-23 | Canon U.S. Life Sciences, Inc. | Methods and systems for dna isolation on a microfluidic device |
| WO2011094577A2 (en) * | 2010-01-29 | 2011-08-04 | Micronics, Inc. | Sample-to-answer microfluidic cartridge |
| GB2483858A (en) | 2010-09-21 | 2012-03-28 | Univ Hull | Amplifying nucleic acids using microfluidic device to perform PRC |
| US20120149088A1 (en) | 2010-11-30 | 2012-06-14 | Life Technologies Corporation | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples |
| CN102146112B (zh) * | 2011-01-25 | 2013-07-10 | 天根生化科技（北京）有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
| WO2013002354A1 (ja) | 2011-06-29 | 2013-01-03 | 株式会社ダナフォーム | 生体試料の前処理方法、ｒｎａの検出方法及び前処理キット |
| WO2013020089A2 (en) * | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
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| EP2958997B1 (en) | 2019-07-17 |
| CA2901641C (en) | 2021-02-09 |
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| CN105164259B (zh) | 2018-02-27 |
| JP2016507242A (ja) | 2016-03-10 |
| CA2901641A1 (en) | 2014-08-28 |
| US10196673B2 (en) | 2019-02-05 |
| JP6567427B2 (ja) | 2019-08-28 |
| EP2958997A1 (en) | 2015-12-30 |
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