ES2735015T3 - Composiciones y métodos para secuenciar polinucleótidos - Google Patents
Composiciones y métodos para secuenciar polinucleótidos Download PDFInfo
- Publication number
- ES2735015T3 ES2735015T3 ES14830903T ES14830903T ES2735015T3 ES 2735015 T3 ES2735015 T3 ES 2735015T3 ES 14830903 T ES14830903 T ES 14830903T ES 14830903 T ES14830903 T ES 14830903T ES 2735015 T3 ES2735015 T3 ES 2735015T3
- Authority
- ES
- Spain
- Prior art keywords
- polynucleotide
- pore
- translocation
- sequence
- levels
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Food Science & Technology (AREA)
- Nanotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361909316P | 2013-11-26 | 2013-11-26 | |
| PCT/US2014/067582 WO2015081178A1 (en) | 2013-11-26 | 2014-11-26 | Compositions and methods for polynucleotide sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2735015T3 true ES2735015T3 (es) | 2019-12-13 |
Family
ID=52424089
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14830903T Active ES2735015T3 (es) | 2013-11-26 | 2014-11-26 | Composiciones y métodos para secuenciar polinucleótidos |
| ES19161148T Active ES2958715T3 (es) | 2013-11-26 | 2014-11-26 | Composiciones y métodos para la secuenciación de polinucleótidos |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES19161148T Active ES2958715T3 (es) | 2013-11-26 | 2014-11-26 | Composiciones y métodos para la secuenciación de polinucleótidos |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US9689033B2 (enExample) |
| EP (3) | EP3074534B1 (enExample) |
| JP (4) | JP6800749B2 (enExample) |
| CN (2) | CN105934522B (enExample) |
| AU (3) | AU2014354726B2 (enExample) |
| CA (2) | CA3157586C (enExample) |
| DK (2) | DK3556869T3 (enExample) |
| ES (2) | ES2735015T3 (enExample) |
| FI (1) | FI3556869T3 (enExample) |
| LT (1) | LT3074534T (enExample) |
| SI (1) | SI3074534T1 (enExample) |
| WO (2) | WO2015081211A2 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| EP3663412B1 (en) * | 2011-09-23 | 2022-03-09 | Oxford Nanopore Technologies PLC | Analysis of a polymer comprising polymer units by means of translocation through a nanopore |
| EP3736339B1 (en) | 2012-02-16 | 2022-07-27 | Oxford Nanopore Technologies plc | Analysis of measurements of a polymer |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| ES2735015T3 (es) * | 2013-11-26 | 2019-12-13 | Illumina Inc | Composiciones y métodos para secuenciar polinucleótidos |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| US10359395B2 (en) | 2014-02-19 | 2019-07-23 | University Of Washington | Nanopore-based analysis of protein characteristics |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| CN119899249A (zh) | 2014-09-01 | 2025-04-29 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| EP3971300B1 (en) | 2014-10-16 | 2024-06-05 | Oxford Nanopore Technologies plc | Sorting of polymers |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| WO2017027518A1 (en) * | 2015-08-10 | 2017-02-16 | Stratos Genomics, Inc. | Single molecule nucleic acid sequencing with molecular sensor complexes |
| CA3002886A1 (en) * | 2015-10-21 | 2017-04-27 | F. Hoffmann-La Roche Ag | Use of fluoropolymers as a hydrophobic layer to support lipid bilayer formation for nanopore based dna sequencing |
| KR102222191B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| CN118272512A (zh) | 2016-04-06 | 2024-07-02 | 牛津纳米孔科技公开有限公司 | 突变体孔 |
| WO2017180141A1 (en) | 2016-04-14 | 2017-10-19 | Lockheed Martin Corporation | Selective interfacial mitigation of graphene defects |
| US10317392B2 (en) * | 2016-06-23 | 2019-06-11 | Roche Sequencing Solutions, Inc. | Formation and calibration of nanopore sequencing cells |
| US10648027B2 (en) | 2016-08-08 | 2020-05-12 | Roche Sequencing Solutions, Inc. | Basecalling for stochastic sequencing processes |
| PL3529380T3 (pl) | 2016-10-19 | 2022-10-24 | Illumina, Inc. | Sposoby chemicznej ligacji kwasów nukleinowych |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| EP3645552B1 (en) | 2017-06-30 | 2023-06-28 | Vib Vzw | Novel protein pores |
| EP3682020B1 (en) * | 2017-09-15 | 2025-09-03 | Illumina, Inc. | Sequence-detection system |
| WO2019055856A1 (en) * | 2017-09-15 | 2019-03-21 | Illumina, Inc. | ACCORDING AND CALIBRATION CHARACTERISTICS OF A SEQUENCE DETECTION SYSTEM |
| US10310959B2 (en) | 2017-11-07 | 2019-06-04 | Bank Of America Corporation | Pre-deployment validation system using intelligent databases |
| WO2019243421A1 (en) * | 2018-06-21 | 2019-12-26 | F. Hoffmann-La Roche Ag | Tunneling junctions for sequencing |
| EP3814529A1 (en) * | 2018-06-26 | 2021-05-05 | Electronic Biosciences Inc. | Controlled nanopore translocation utilizing extremophilic replication proteins |
| MY204234A (en) | 2018-08-27 | 2024-08-16 | Regeneron Pharma | Use of raman spectroscopy in downstream purification |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| WO2020084705A1 (ja) * | 2018-10-24 | 2020-04-30 | 株式会社日立ハイテク | 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法 |
| GB201907244D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| IL291787A (en) | 2019-09-29 | 2022-07-01 | Qitan Tech Ltd Beijing | mmup monomer variant and its application |
| IL293024B1 (en) * | 2019-11-19 | 2026-07-01 | Univ Groningen | Artificial insemination, methods and uses |
| NL2024579B1 (en) * | 2019-12-24 | 2021-09-06 | Univ Delft Tech | Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes |
| CN114807317A (zh) * | 2021-01-22 | 2022-07-29 | 上海羿鸣生物科技有限公司 | 一种优化的dna线性扩增方法及试剂盒 |
| GB202107192D0 (en) * | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| CN113322180B (zh) * | 2021-05-31 | 2022-07-15 | 中国科学院物理研究所 | 基于纳米孔测序的力谱分析方法和分析装置 |
| WO2023049682A1 (en) | 2021-09-22 | 2023-03-30 | Illumina, Inc. | Sequencing polynucleotides using nanopores |
| US20230298693A1 (en) * | 2022-02-28 | 2023-09-21 | Rajant Health Incorporated | Alignment-free variant calling |
| CN120435657A (zh) | 2022-10-28 | 2025-08-05 | 格罗宁根大学 | 蛋白质的基于纳米孔的分析 |
| WO2024220969A2 (en) * | 2023-04-21 | 2024-10-24 | Arizona Board Of Regents On Behalf Of Arizona State University | Novel transmembrane sensors and method of characterization for lysis-free detection of intracellular targets |
| WO2026073207A1 (en) | 2024-09-30 | 2026-04-02 | Illumina, Inc. | Affinity-binder-based assay compositions and methods |
| CN119639558B (zh) * | 2024-12-13 | 2025-09-26 | 杭州海兰时生物科技有限责任公司 | 一种用于单细胞测序的液滴融合系统 |
| WO2026143014A1 (en) | 2024-12-23 | 2026-07-02 | Illumina, Inc. | Affinity-binder-based assay compositions and methods for spatial proteomics |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
| US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
| US5637684A (en) | 1994-02-23 | 1997-06-10 | Isis Pharmaceuticals, Inc. | Phosphoramidate and phosphorothioamidate oligomeric compounds |
| US5508179A (en) | 1994-03-18 | 1996-04-16 | Bio-Rad Laboratories, Inc. | Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions |
| US5795782A (en) * | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| EP1192103A1 (en) | 1999-06-22 | 2002-04-03 | President And Fellows of Harvard College | Control of solid state dimensional features |
| US6913884B2 (en) | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| JP2003521252A (ja) | 2000-02-07 | 2003-07-15 | イルミナ インコーポレイテッド | ユニバーサルプライミングを用いる核酸検出方法 |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| EP2122344B8 (en) | 2007-02-20 | 2019-08-21 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor system |
| US20110229877A1 (en) * | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| DE202009019021U1 (de) | 2008-09-22 | 2015-09-24 | University Of Washington | Msp-nanoporen |
| GB0905140D0 (en) * | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| EP2614156B1 (en) | 2010-09-07 | 2018-08-01 | The Regents of The University of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| WO2013016486A1 (en) | 2011-07-27 | 2013-01-31 | The Board Of Trustees Of The University Of Illinois | Nanopore sensors for biomolecular characterization |
| CN104039979B (zh) * | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| CA2861808C (en) | 2011-12-29 | 2021-02-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| JP5809572B2 (ja) | 2012-01-30 | 2015-11-11 | ルネサスエレクトロニクス株式会社 | 半導体装置 |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| GB2559073A (en) * | 2012-06-08 | 2018-07-25 | Pacific Biosciences California Inc | Modified base detection with nanopore sequencing |
| AU2013291763B2 (en) * | 2012-07-19 | 2019-06-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| ES2735015T3 (es) * | 2013-11-26 | 2019-12-13 | Illumina Inc | Composiciones y métodos para secuenciar polinucleótidos |
-
2014
- 2014-11-26 ES ES14830903T patent/ES2735015T3/es active Active
- 2014-11-26 EP EP14830903.2A patent/EP3074534B1/en active Active
- 2014-11-26 FI FIEP19161148.2T patent/FI3556869T3/fi active
- 2014-11-26 CN CN201480064725.5A patent/CN105934522B/zh active Active
- 2014-11-26 WO PCT/US2014/067639 patent/WO2015081211A2/en not_active Ceased
- 2014-11-26 EP EP19161148.2A patent/EP3556869B1/en active Active
- 2014-11-26 DK DK19161148.2T patent/DK3556869T3/da active
- 2014-11-26 JP JP2016521718A patent/JP6800749B2/ja active Active
- 2014-11-26 CA CA3157586A patent/CA3157586C/en active Active
- 2014-11-26 CN CN202110859489.8A patent/CN113528632A/zh active Pending
- 2014-11-26 AU AU2014354726A patent/AU2014354726B2/en active Active
- 2014-11-26 DK DK14830903.2T patent/DK3074534T3/da active
- 2014-11-26 WO PCT/US2014/067582 patent/WO2015081178A1/en not_active Ceased
- 2014-11-26 SI SI201431264T patent/SI3074534T1/sl unknown
- 2014-11-26 US US14/554,741 patent/US9689033B2/en active Active
- 2014-11-26 CA CA2926871A patent/CA2926871C/en active Active
- 2014-11-26 ES ES19161148T patent/ES2958715T3/es active Active
- 2014-11-26 EP EP23188555.9A patent/EP4282983A3/en active Pending
- 2014-11-26 LT LTEP14830903.2T patent/LT3074534T/lt unknown
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2017
- 2017-05-26 US US15/606,354 patent/US10364462B2/en active Active
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2019
- 2019-06-12 US US16/439,022 patent/US11041196B2/en active Active
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2020
- 2020-04-14 AU AU2020202489A patent/AU2020202489B2/en active Active
- 2020-11-25 JP JP2020195453A patent/JP7349972B2/ja active Active
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2021
- 2021-05-25 US US17/329,482 patent/US11879155B2/en active Active
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2022
- 2022-09-16 AU AU2022231786A patent/AU2022231786B2/en active Active
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2023
- 2023-05-30 JP JP2023088480A patent/JP2023116553A/ja active Pending
- 2023-12-08 US US18/533,863 patent/US20240167085A1/en active Pending
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2025
- 2025-02-05 JP JP2025017268A patent/JP2025072503A/ja active Pending
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