ES2680196A1 - Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables - Google Patents
Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables Download PDFInfo
- Publication number
- ES2680196A1 ES2680196A1 ES201730113A ES201730113A ES2680196A1 ES 2680196 A1 ES2680196 A1 ES 2680196A1 ES 201730113 A ES201730113 A ES 201730113A ES 201730113 A ES201730113 A ES 201730113A ES 2680196 A1 ES2680196 A1 ES 2680196A1
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- polypeptides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables. La invención se refiere a polipéptidos con actividad polisacárido monooxigenasa, a una célula hospedadora que los expresa, preferiblemente una célula de Myceliophthora thermophila que expresa de manera recombinante al menos uno de dichos polipéptidos, a una composición enzimática que comprende al menos uno de dichos polipéptidos, preferiblemente junto con otras enzimas celulolíticas, al uso de esta célula hospedadora, de al menos uno de los polipéptidos con actividad polisacárido monooxigenasa o de la composición enzimática para la degradación de biomasa celulósica y a un procedimiento para producir bioproductos, preferiblemente bioetanol, que comprende el uso de dicha célula hospedadora, de al menos uno de los polipéptidos de la invención o de la composición enzimática de la invención.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201730113A ES2680196B1 (es) | 2017-02-01 | 2017-02-01 | Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables |
EP18748611.3A EP3578648A4 (en) | 2017-02-01 | 2018-01-12 | POLYPEPTIDES WITH POLYSACCHARIDE MONOOXYGENASE ACTIVITY AND USE THEREOF FOR THE PRODUCTION OF FERMENTABLE SUGARS |
US16/481,815 US20200165580A1 (en) | 2017-02-01 | 2018-01-12 | Polypeptides with polysaccharide monooxygenase activity and use thereof for the production of fermentable sugars |
PCT/ES2018/070023 WO2018142002A1 (es) | 2017-02-01 | 2018-01-12 | Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201730113A ES2680196B1 (es) | 2017-02-01 | 2017-02-01 | Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2680196A1 true ES2680196A1 (es) | 2018-09-04 |
ES2680196B1 ES2680196B1 (es) | 2019-07-30 |
Family
ID=63039372
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201730113A Expired - Fee Related ES2680196B1 (es) | 2017-02-01 | 2017-02-01 | Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables |
Country Status (4)
Country | Link |
---|---|
US (1) | US20200165580A1 (es) |
EP (1) | EP3578648A4 (es) |
ES (1) | ES2680196B1 (es) |
WO (1) | WO2018142002A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR112013002377B1 (pt) * | 2010-08-06 | 2020-12-29 | Novozymes A/S | método para degradar ou hidrolisar um polissacarídeo, para produzir sacarídeos solúveis e para produzir uma substância orgânica, e, processo para produzir um produto de fermentação |
US9150842B2 (en) * | 2011-03-09 | 2015-10-06 | Novozymes A/S | Methods of increasing the cellulolytic enhancing activity of a polypeptide |
US20150210991A1 (en) * | 2012-09-19 | 2015-07-30 | Novozymes, Inc. | Methods For Enhancing The Degradation Or Conversion Of Cellulosic Material |
BR112015029081A2 (pt) * | 2013-05-20 | 2017-09-19 | Abengoa Bioenergia Nuevas Tecnologias Sa | Célula hospedeira myceliophthora thermophila expressando uma enzima beta-xilosidase recombinante, enzima beta-xilosidase recombinante expressada por meio da mesma, composição, uso de célula hospedeira, enzima beta-xilosidase ou composição e métodos de produção de açúcares fermentescíveis e de produção de bioproduto a partir de biomassa |
US10479983B2 (en) * | 2013-07-29 | 2019-11-19 | Danisco Us Inc | Variant enzymes |
EP3315609A1 (en) * | 2015-06-23 | 2018-05-02 | Abengoa Bioenergía Nuevas Tecnologías, S. A. | Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity |
-
2017
- 2017-02-01 ES ES201730113A patent/ES2680196B1/es not_active Expired - Fee Related
-
2018
- 2018-01-12 EP EP18748611.3A patent/EP3578648A4/en not_active Withdrawn
- 2018-01-12 WO PCT/ES2018/070023 patent/WO2018142002A1/es unknown
- 2018-01-12 US US16/481,815 patent/US20200165580A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2018142002A1 (es) | 2018-08-09 |
US20200165580A1 (en) | 2020-05-28 |
EP3578648A1 (en) | 2019-12-11 |
EP3578648A4 (en) | 2020-12-09 |
ES2680196B1 (es) | 2019-07-30 |
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