ES2680196A1 - Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables - Google Patents

Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables Download PDF

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Publication number
ES2680196A1
ES2680196A1 ES201730113A ES201730113A ES2680196A1 ES 2680196 A1 ES2680196 A1 ES 2680196A1 ES 201730113 A ES201730113 A ES 201730113A ES 201730113 A ES201730113 A ES 201730113A ES 2680196 A1 ES2680196 A1 ES 2680196A1
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Prior art keywords
polypeptides
translation
production
machine
legally binding
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Granted
Application number
ES201730113A
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ES2680196B1 (es
Inventor
Lucía MARTÍN PÉREZ
Bruno DÍEZ GARCÍA
Francisco Manuel REYES SOSA
Noelia VALBUENA CRESPO
Antonio Javier MORENO PÉREZ
Dolores PÉREZ GÓMEZ
Ana Isabel PLATERO GÓMEZ
Sandra GAVALDÁ MARTÍN
Laura VIÑAS DE LA CRUZ
Javier ROCHA MARTÍN
Laura SÁNCHEZ ZAMORANO
María De Los Angeles BERMÚDEZ ALCÁNTARA
Laura LEDESMA GARCÍA
Juan Luis RAMOS MARTÍN
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Abengoa Bioenergia Nuevas Technologias SA
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Abengoa Bioenergia Nuevas Technologias SA
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Priority to ES201730113A priority Critical patent/ES2680196B1/es
Priority to US16/481,815 priority patent/US20200165580A1/en
Priority to EP18748611.3A priority patent/EP3578648A4/en
Priority to PCT/ES2018/070023 priority patent/WO2018142002A1/es
Publication of ES2680196A1 publication Critical patent/ES2680196A1/es
Application granted granted Critical
Publication of ES2680196B1 publication Critical patent/ES2680196B1/es
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables. La invención se refiere a polipéptidos con actividad polisacárido monooxigenasa, a una célula hospedadora que los expresa, preferiblemente una célula de Myceliophthora thermophila que expresa de manera recombinante al menos uno de dichos polipéptidos, a una composición enzimática que comprende al menos uno de dichos polipéptidos, preferiblemente junto con otras enzimas celulolíticas, al uso de esta célula hospedadora, de al menos uno de los polipéptidos con actividad polisacárido monooxigenasa o de la composición enzimática para la degradación de biomasa celulósica y a un procedimiento para producir bioproductos, preferiblemente bioetanol, que comprende el uso de dicha célula hospedadora, de al menos uno de los polipéptidos de la invención o de la composición enzimática de la invención.
ES201730113A 2017-02-01 2017-02-01 Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables Expired - Fee Related ES2680196B1 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES201730113A ES2680196B1 (es) 2017-02-01 2017-02-01 Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables
US16/481,815 US20200165580A1 (en) 2017-02-01 2018-01-12 Polypeptides with polysaccharide monooxygenase activity and use thereof for the production of fermentable sugars
EP18748611.3A EP3578648A4 (en) 2017-02-01 2018-01-12 POLYPEPTIDES WITH POLYSACCHARIDE MONOOXYGENASE ACTIVITY AND USE THEREOF FOR THE PRODUCTION OF FERMENTABLE SUGARS
PCT/ES2018/070023 WO2018142002A1 (es) 2017-02-01 2018-01-12 Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201730113A ES2680196B1 (es) 2017-02-01 2017-02-01 Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables

Publications (2)

Publication Number Publication Date
ES2680196A1 true ES2680196A1 (es) 2018-09-04
ES2680196B1 ES2680196B1 (es) 2019-07-30

Family

ID=63039372

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201730113A Expired - Fee Related ES2680196B1 (es) 2017-02-01 2017-02-01 Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables

Country Status (4)

Country Link
US (1) US20200165580A1 (es)
EP (1) EP3578648A4 (es)
ES (1) ES2680196B1 (es)
WO (1) WO2018142002A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013002377B1 (pt) * 2010-08-06 2020-12-29 Novozymes A/S método para degradar ou hidrolisar um polissacarídeo, para produzir sacarídeos solúveis e para produzir uma substância orgânica, e, processo para produzir um produto de fermentação
EP3339442B1 (en) * 2011-03-09 2022-05-11 Novozymes A/S Methods of increasing the cellulolytic enhancing activity of a polypeptide
EP2898068A2 (en) * 2012-09-19 2015-07-29 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
ES2727390T3 (es) * 2013-05-20 2019-10-15 Abengoa Bioenergia Nuevas Tecnologias Sa Expresión de enzimas beta-xilosidasas recombinantes
JP2016533741A (ja) * 2013-07-29 2016-11-04 ダニスコ・ユーエス・インク 酵素の変異体
EP3315609A1 (en) * 2015-06-23 2018-05-02 Abengoa Bioenergía Nuevas Tecnologías, S. A. Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity

Also Published As

Publication number Publication date
EP3578648A1 (en) 2019-12-11
WO2018142002A1 (es) 2018-08-09
ES2680196B1 (es) 2019-07-30
EP3578648A4 (en) 2020-12-09
US20200165580A1 (en) 2020-05-28

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