ES2628301T3 - Recombinación de virus de gripe - Google Patents
Recombinación de virus de gripe Download PDFInfo
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- ES2628301T3 ES2628301T3 ES13712156.2T ES13712156T ES2628301T3 ES 2628301 T3 ES2628301 T3 ES 2628301T3 ES 13712156 T ES13712156 T ES 13712156T ES 2628301 T3 ES2628301 T3 ES 2628301T3
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- C—CHEMISTRY; METALLURGY
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- C—CHEMISTRY; METALLURGY
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Landscapes
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| WO2013032942A1 (en) | 2011-08-26 | 2013-03-07 | Yoshihiro Kawaoka | Influenza viruses with mutant pb2 gene segment as live attenuated vaccines |
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| CA2898961A1 (en) * | 2013-01-23 | 2014-07-31 | Novartis Ag | Influenza virus reassortment |
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| WO2015196150A2 (en) | 2014-06-20 | 2015-12-23 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| RU2605317C1 (ru) * | 2015-06-04 | 2016-12-20 | Федеральное государственное автономное образовательное учреждение высшего образования "Новосибирский национальный исследовательский государственный университет" (Новосибирский государственный университет, НГУ) | АДАПТИРОВАННЫЕ ПАНДЕМИЧЕСКИЕ ШТАММЫ ВИРУСА ГРИППА A/Tomsk/273/2010-MA1(H1N1pdm09), A/Tomsk/273/2010-MA2(H1N1pdm09) И A/Tomsk/273/2010-MA3(H1N1pdm09) ДЛЯ ОЦЕНКИ ДЕЙСТВИЯ ПРОТИВОВИРУСНЫХ ПРЕПАРАТОВ (ВАРИАНТЫ) |
| HK1254344A1 (zh) | 2015-06-26 | 2019-07-19 | Seqirus UK Limited | 抗原匹配的流感疫苗 |
| JP6830070B2 (ja) | 2015-07-07 | 2021-02-17 | セキラス ユーケー リミテッドＳｅｑｉｒｕｓ ＵＫ Ｌｉｍｉｔｅｄ | インフルエンザ効力アッセイ |
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| CN111032861B (zh) * | 2017-08-28 | 2023-09-12 | 一般财团法人阪大微生物病研究会 | 重配流感病毒的阶段性制备方法 |
| JP2021500891A (ja) | 2017-10-25 | 2021-01-14 | ウィスコンシン アルムニ リサーチ ファンデイション | 卵における複製のための安定化されたｈａを有する組換えインフルエンザウイルス |
| CN111511800B (zh) | 2017-10-30 | 2023-11-28 | 武田药品工业株式会社 | 灭活脂包膜病毒的环境相容性去污剂 |
| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
| JP7655849B2 (ja) | 2018-08-20 | 2025-04-02 | ウィスコンシン アルムニ リサーチ ファンデイション | ヘマグルチニン（ｈａ）タンパク質内の非ドミナントエピトープに対する免疫応答を誘起するためのベクター |
| EP3921413A1 (en) | 2019-02-08 | 2021-12-15 | Wisconsin Alumni Research Foundation (WARF) | Humanized cell line |
| US11390649B2 (en) | 2019-05-01 | 2022-07-19 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| WO2021041624A2 (en) | 2019-08-27 | 2021-03-04 | Yoshihiro Kawaoka | Recombinant influenza viruses with stabilized ha for replication in eggs |
| JP2023511444A (ja) | 2020-01-24 | 2023-03-17 | ウィスコンシン アルムニ リサーチ ファンデイション | 安定化されたｎａを有する組換えインフルエンザウイルス |
| US12290562B2 (en) | 2020-03-25 | 2025-05-06 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
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| US11694876B2 (en) | 2021-12-08 | 2023-07-04 | Applied Materials, Inc. | Apparatus and method for delivering a plurality of waveform signals during plasma processing |
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| HU212924B (en) | 1989-05-25 | 1996-12-30 | Chiron Corp | Adjuvant formulation comprising a submicron oil droplet emulsion |
| WO1995011700A1 (en) | 1993-10-29 | 1995-05-04 | Pharmos Corp. | Submicron emulsions as vaccine adjuvants |
| DE19612966B4 (de) | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| DE19612967A1 (de) | 1996-04-01 | 1997-10-02 | Behringwerke Ag | Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren |
| TW570803B (en) | 1997-04-09 | 2004-01-11 | Duphar Int Res | Influenza vaccine |
| US6080725A (en) | 1997-05-20 | 2000-06-27 | Galenica Pharmaceuticals, Inc. | Immunostimulating and vaccine compositions employing saponin analog adjuvants and uses thereof |
| DK1098961T3 (da) | 1998-06-12 | 2008-05-26 | Andrej Y Dr Egorov | Gensplejsede svækkede vira, der inducerer interferon |
| DE60036952T2 (de) | 1999-09-24 | 2008-08-07 | Glaxosmithkline Biologicals S.A. | Influenzavirus-impfstoffzusammensetzung zur nasalen anwendung |
| AU2001236042B9 (en) | 2000-03-03 | 2006-06-29 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Cell usable in serum-free culture and suspension culture and process for producing virus for vaccine by using the cell |
| GB0024089D0 (en) | 2000-10-02 | 2000-11-15 | Smithkline Beecham Biolog | Novel compounds |
| US20040071734A1 (en) | 2001-02-23 | 2004-04-15 | Nathalie Garcon | Novel vaccine |
| EP1361889A1 (en) | 2001-02-23 | 2003-11-19 | GlaxoSmithKline Biologicals S.A. | Novel vaccine |
| MY134424A (en) | 2001-05-30 | 2007-12-31 | Saechsisches Serumwerk | Stable influenza virus preparations with low or no amount of thiomersal |
| KR100908757B1 (ko) * | 2003-05-28 | 2009-07-22 | 위스콘신 얼럼나이 리서어치 화운데이션 | 백신 및 유전자 요법을 위한 고 역가 재조합 인플루엔자바이러스 |
| KR20110132416A (ko) | 2004-04-05 | 2011-12-07 | 화이자 프로덕츠 인코포레이티드 | 미세유체화된 수중유 유화액 및 백신 조성물 |
| WO2005113756A1 (en) | 2004-05-14 | 2005-12-01 | Glaxosmithkline Biologicals S.A. | Method |
| WO2005113758A1 (en) | 2004-05-20 | 2005-12-01 | Id Biomedical Corporation | Process for the production of an influenza vaccine |
| US7670837B2 (en) | 2004-12-23 | 2010-03-02 | Medimmune, Llc | Non-tumorigenic MDCK cell line for propagating viruses |
| US7691368B2 (en) | 2005-04-15 | 2010-04-06 | Merial Limited | Vaccine formulations |
| AU2006262380A1 (en) | 2005-06-21 | 2007-01-04 | Medimmune, Llc | Methods and compositions for expressing a heterologous protease |
| US8703095B2 (en) | 2005-07-07 | 2014-04-22 | Sanofi Pasteur S.A. | Immuno-adjuvant emulsion |
| DE602006019629D1 (de) | 2005-11-01 | 2011-02-24 | Novartis Vaccines & Diagnostic | Von zellen stammende virale impfstoffe mit geringen mengen an rest-zell-dna |
| DE202006021242U1 (de) | 2005-11-04 | 2014-01-29 | Novartis Vaccines And Diagnostics S.R.L. | Emulsionen mit freiem wässrigen Phasen Tensid als Adjuvans für Spalt-Grippeimpfstoffe |
| FR2896162B1 (fr) | 2006-01-13 | 2008-02-15 | Sanofi Pasteur Sa | Emulsion huile dans eau thermoreversible |
| EP2010557B1 (en) * | 2006-03-31 | 2014-02-26 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines |
| PT2016194E (pt) | 2006-04-19 | 2015-07-20 | Medimmune Llc | Métodos e composições para expressar arn viral de polaridade negativa em células caninas |
| US20100183667A1 (en) | 2006-10-12 | 2010-07-22 | Glaxo Smithkline Biologicals S.A. | Vaccine Comprising an Oil in Water Emulsion Adjuvant |
| CN101553252A (zh) | 2006-12-06 | 2009-10-07 | 诺华有限公司 | 包含来自于四株流感病毒的抗原的疫苗 |
| EP2045323A1 (en) | 2007-10-05 | 2009-04-08 | Avir Green Hills Biotechnology Research Development Trade Ag | Linear expression constructs for production of influenza virus particles |
| CN107365751B (zh) * | 2008-12-16 | 2021-07-09 | 纳米医疗公司 | 流感疫苗的生产 |
| EP2233152A1 (en) * | 2009-03-24 | 2010-09-29 | Avir Green Hills Biotechnology Research Development Trade Ag | High growth reassortant influenza A virus |
| US9821052B2 (en) | 2009-07-31 | 2017-11-21 | Seqirus UK Limited | Reverse genetics systems |
| US9109013B2 (en) * | 2009-10-26 | 2015-08-18 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses with enhanced replication in vero cells |
| WO2013032942A1 (en) * | 2011-08-26 | 2013-03-07 | Yoshihiro Kawaoka | Influenza viruses with mutant pb2 gene segment as live attenuated vaccines |
-
2013
- 2013-03-02 AU AU2013205478A patent/AU2013205478B9/en active Active
- 2013-03-02 JP JP2013548864A patent/JP6054883B2/ja active Active
- 2013-03-02 EP EP13712156.2A patent/EP2820126B1/en active Active
- 2013-03-02 ES ES13712156.2T patent/ES2628301T3/es active Active
- 2013-03-02 CN CN201380000396.3A patent/CN103608453B/zh active Active
- 2013-03-02 CA CA2813723A patent/CA2813723A1/en not_active Abandoned
- 2013-03-02 WO PCT/EP2013/054227 patent/WO2013087945A2/en not_active Ceased
- 2013-06-03 US US13/909,013 patent/US9422528B2/en active Active
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2015
- 2015-03-19 JP JP2015055989A patent/JP6279503B2/ja active Active
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2016
- 2016-07-14 US US15/210,485 patent/US10245312B2/en active Active
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2019
- 2019-02-11 US US16/272,018 patent/US20200000909A1/en not_active Abandoned
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| Publication number | Publication date |
|---|---|
| WO2013087945A2 (en) | 2013-06-20 |
| EP2820126A2 (en) | 2015-01-07 |
| CN103608453B (zh) | 2018-07-24 |
| US20170095549A1 (en) | 2017-04-06 |
| US10245312B2 (en) | 2019-04-02 |
| JP6054883B2 (ja) | 2016-12-27 |
| WO2013087945A3 (en) | 2013-11-14 |
| EP2820126B1 (en) | 2017-05-17 |
| US20200000909A1 (en) | 2020-01-02 |
| JP2014506133A (ja) | 2014-03-13 |
| US9422528B2 (en) | 2016-08-23 |
| AU2013205478B2 (en) | 2014-06-26 |
| CN103608453A (zh) | 2014-02-26 |
| AU2013205478A1 (en) | 2013-06-06 |
| JP2015119730A (ja) | 2015-07-02 |
| US20140030291A1 (en) | 2014-01-30 |
| AU2013205478B9 (en) | 2014-11-20 |
| CA2813723A1 (en) | 2013-09-02 |
| JP6279503B2 (ja) | 2018-02-14 |
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