ES2626646T3 - Método y aparato para detectar micropartículas en muestras de fluidos - Google Patents
Método y aparato para detectar micropartículas en muestras de fluidos Download PDFInfo
- Publication number
- ES2626646T3 ES2626646T3 ES10011461.0T ES10011461T ES2626646T3 ES 2626646 T3 ES2626646 T3 ES 2626646T3 ES 10011461 T ES10011461 T ES 10011461T ES 2626646 T3 ES2626646 T3 ES 2626646T3
- Authority
- ES
- Spain
- Prior art keywords
- fluorescent substance
- paragraph
- electromagnetic radiation
- microparticles
- capillary tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000011859 microparticle Substances 0.000 title abstract description 28
- 239000012530 fluid Substances 0.000 title abstract description 14
- 238000000034 method Methods 0.000 title description 4
- 239000000126 substance Substances 0.000 abstract description 26
- 230000005670 electromagnetic radiation Effects 0.000 abstract description 11
- 238000001514 detection method Methods 0.000 abstract description 7
- 241000700141 Rotifera Species 0.000 description 19
- 239000011049 pearl Substances 0.000 description 16
- 239000000523 sample Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 10
- FHIVAFMUCKRCQO-UHFFFAOYSA-N diazinon Chemical compound CCOP(=S)(OCC)OC1=CC(C)=NC(C(C)C)=N1 FHIVAFMUCKRCQO-UHFFFAOYSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000013068 control sample Substances 0.000 description 4
- 230000008030 elimination Effects 0.000 description 4
- 238000003379 elimination reaction Methods 0.000 description 4
- 239000011324 bead Substances 0.000 description 3
- 230000037406 food intake Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000004899 motility Effects 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 210000004970 cd4 cell Anatomy 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000700104 Brachionus plicatilis Species 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Farming Of Fish And Shellfish (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4901297P | 1997-06-09 | 1997-06-09 | |
| US49012P | 1997-06-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2626646T3 true ES2626646T3 (es) | 2017-07-25 |
Family
ID=21957617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10011461.0T Expired - Lifetime ES2626646T3 (es) | 1997-06-09 | 1998-06-09 | Método y aparato para detectar micropartículas en muestras de fluidos |
Country Status (6)
| Country | Link |
|---|---|
| EP (2) | EP2302368B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP4754661B2 (cg-RX-API-DMAC7.html) |
| AU (1) | AU7831798A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2293426C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2626646T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO1998057152A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2264428B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| US20020028434A1 (en) | 2000-09-06 | 2002-03-07 | Guava Technologies, Inc. | Particle or cell analyzer and method |
| JP4685229B2 (ja) * | 2000-10-31 | 2011-05-18 | オリンパス株式会社 | レーザ顕微鏡 |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| DE60126775T2 (de) * | 2001-10-10 | 2007-10-31 | Borealis Technology Oy | Teilchendatenanalyse |
| NZ538462A (en) | 2002-08-01 | 2008-06-30 | Xy Inc | Low pressure sperm cell separation system |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| JP2005535346A (ja) | 2002-08-15 | 2005-11-24 | エックスワイ，インコーポレイテッド | 高分解能フローサイトメーター |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| ES2930062T3 (es) | 2003-03-28 | 2022-12-05 | Inguran Llc | Aparato para detectar el punto de rotura de un sistema de generación de gotitas |
| EP1625203B1 (en) | 2003-05-15 | 2015-04-08 | Xy, Llc | Efficient haploid cell sorting for flow cytometer systems |
| AU2005228893B2 (en) | 2004-03-29 | 2010-08-19 | Inguran, Llc | Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations |
| EP1769067A2 (en) | 2004-07-22 | 2007-04-04 | Monsanto Technology LLC | Process for enriching a population of sperm cells |
| EP2085762B1 (en) * | 2008-01-30 | 2018-07-04 | Palo Alto Research Center Incorporated | Transmitting/reflecting emanating light with time variation |
| CN101672841B (zh) * | 2008-09-09 | 2013-05-08 | 北京万德高科技发展有限公司 | 用于生物样品的检测仪器和检测方法 |
| EP2633284B1 (en) * | 2010-10-25 | 2021-08-25 | Accuri Cytometers, Inc. | Systems and user interface for collecting a data set in a flow cytometer |
| BR112014001238A2 (pt) | 2011-07-19 | 2017-06-13 | Ovizio Imaging Systems N V | método para a detecção de células cancerosas e/ou classificação de células numa amostra de células de líquido; sistema para a detecção de células e/ou a classificação de células cancerosas numa amostra de células; método de atualização e/ou melhoria de uma base de dados que compreende os limiares ligados á informação holográfica; e base de dados de objetos |
| EP2594334A1 (en) | 2011-11-21 | 2013-05-22 | Drive O2 | Sample vial for digital holographic analysis of a liquid cell sample |
| EP2626686A1 (en) | 2012-02-13 | 2013-08-14 | Ovizio Imaging Systems NV/SA | Flow cytometer with digital holographic microscope |
| US9904248B2 (en) | 2012-09-20 | 2018-02-27 | Ovizio Imaging Systems NV/SA | Digital holographic microscope with fluid systems |
| EP3196631A1 (en) | 2016-01-19 | 2017-07-26 | Ovizio Imaging Systems NV/SA | Digital holographic microscope with electro-fluidic system, said electro-fluidic system and methods of use |
| CN109270032B (zh) * | 2018-08-20 | 2021-08-20 | 杭州腾海科技有限公司 | 一种水体藻类在线监测装置 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2732272C2 (de) | 1977-07-16 | 1979-07-05 | Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts, 6900 Heidelberg | Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefärbten Partikeln, insbesondere biologischen Zellen |
| US4172227A (en) | 1978-07-21 | 1979-10-23 | Becton, Dickinson And Company | Flow microfluorometer |
| EP0068404B1 (en) * | 1981-06-24 | 1985-10-02 | Becton Dickinson and Company | Analyzer for simultaneously determining volume and light emission characteristics of particles |
| DE3574617D1 (de) * | 1984-09-11 | 1990-01-11 | Partec Ag | Verfahren und vorrichtung zur sortierung von mikroskopischen partikeln. |
| FI844027A7 (fi) | 1984-10-12 | 1986-04-13 | Labsystems Oy | Immunologiskt bestaemningsfoerfarande. |
| US4756884A (en) * | 1985-08-05 | 1988-07-12 | Biotrack, Inc. | Capillary flow device |
| US4668868A (en) | 1986-02-20 | 1987-05-26 | Noller Hans T | Apparatus for performing fluoroimmunoassays of biological specimens |
| JPS63231244A (ja) * | 1987-03-20 | 1988-09-27 | Hitachi Ltd | 細胞分析装置 |
| DE3715114A1 (de) | 1987-05-06 | 1988-11-17 | Krause Hans | Verfahren und einrichtung zum toxizitaetsnachweis in oberflaechengewaessern sowie in trink- und brauchwasser |
| DE3718407A1 (de) * | 1987-06-02 | 1988-12-22 | Hund Helmut Gmbh | Anordnung fuer die optische analyse von partikelpopulationen in gasen und fluessigkeiten |
| US4838688A (en) * | 1987-10-16 | 1989-06-13 | Robert Rhoads | High sensitivity chromatography detector |
| JPH02145941A (ja) * | 1988-11-28 | 1990-06-05 | Hitachi Ltd | 細胞分析装置 |
| US4979824A (en) * | 1989-05-26 | 1990-12-25 | Board Of Trustees Of The Leland Stanford Junior University | High sensitivity fluorescent single particle and single molecule detection apparatus and method |
| DK111990D0 (da) * | 1990-05-04 | 1990-05-04 | Biometic Aps | Apparat og fremgangsmaade til analyse af en vaeskesuspension |
| JPH04102062A (ja) * | 1990-08-22 | 1992-04-03 | Hitachi Ltd | 粒子免疫側定法 |
| JP3102938B2 (ja) * | 1991-12-30 | 2000-10-23 | シスメックス株式会社 | 粒子画像分析装置 |
| JP2756397B2 (ja) * | 1992-02-20 | 1998-05-25 | キヤノン株式会社 | 粒子操作方法及び装置、並びにこれを用いた測定装置 |
| JP3076144B2 (ja) * | 1992-05-01 | 2000-08-14 | キヤノン株式会社 | 生体微量成分検査装置 |
| US5430541A (en) * | 1993-01-12 | 1995-07-04 | Applied Biosystems Inc. | High efficiency fluorescence flow cell for capillary liquid chromatography or capillary electrophoresis |
| US5585246A (en) | 1993-02-17 | 1996-12-17 | Biometric Imaging, Inc. | Method for preparing a sample in a scan capillary for immunofluorescent interrogation |
| NO932088L (no) * | 1993-06-08 | 1995-01-05 | Oddbjoern Gjelsnes | Anordning for anvendelse ved væskeströmscytometri |
| JPH07286953A (ja) * | 1994-04-19 | 1995-10-31 | Toa Medical Electronics Co Ltd | イメージングフローサイトメータ |
| JPH08163996A (ja) * | 1994-12-13 | 1996-06-25 | Sumitomo Bakelite Co Ltd | 動物細胞毒性試験方法 |
| FI98765C (fi) * | 1995-01-16 | 1997-08-11 | Erkki Soini | Virtaussytometrinen menetelmä ja laite |
| DE19520298A1 (de) * | 1995-06-02 | 1996-12-05 | Bayer Ag | Sortiervorrichtung für biologische Zellen oder Viren |
| US5726751A (en) * | 1995-09-27 | 1998-03-10 | University Of Washington | Silicon microchannel optical flow cytometer |
-
1998
- 1998-06-09 EP EP10011461.0A patent/EP2302368B1/en not_active Expired - Lifetime
- 1998-06-09 ES ES10011461.0T patent/ES2626646T3/es not_active Expired - Lifetime
- 1998-06-09 CA CA002293426A patent/CA2293426C/en not_active Expired - Lifetime
- 1998-06-09 EP EP98926490.8A patent/EP0988523B1/en not_active Expired - Lifetime
- 1998-06-09 JP JP50311999A patent/JP4754661B2/ja not_active Expired - Lifetime
- 1998-06-09 AU AU78317/98A patent/AU7831798A/en not_active Abandoned
- 1998-06-09 WO PCT/US1998/011958 patent/WO1998057152A1/en not_active Ceased
-
2009
- 2009-09-14 JP JP2009211291A patent/JP4999900B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0988523B1 (en) | 2013-08-14 |
| CA2293426A1 (en) | 1998-12-17 |
| CA2293426C (en) | 2007-05-15 |
| JP2010017188A (ja) | 2010-01-28 |
| EP2302368B1 (en) | 2017-04-05 |
| JP2002506522A (ja) | 2002-02-26 |
| JP4754661B2 (ja) | 2011-08-24 |
| EP2302368A1 (en) | 2011-03-30 |
| AU7831798A (en) | 1998-12-30 |
| WO1998057152A1 (en) | 1998-12-17 |
| EP0988523A1 (en) | 2000-03-29 |
| JP4999900B2 (ja) | 2012-08-15 |
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