ES2623780T3 - Sistemas de expresión de baculovirus mejorados - Google Patents
Sistemas de expresión de baculovirus mejorados Download PDFInfo
- Publication number
- ES2623780T3 ES2623780T3 ES12745446.0T ES12745446T ES2623780T3 ES 2623780 T3 ES2623780 T3 ES 2623780T3 ES 12745446 T ES12745446 T ES 12745446T ES 2623780 T3 ES2623780 T3 ES 2623780T3
- Authority
- ES
- Spain
- Prior art keywords
- baculovirus
- raav
- baculovirus expression
- gene
- expression systems
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000701447 unidentified baculovirus Species 0.000 title abstract description 18
- 101710181863 Structural DNA-binding protein p10 Proteins 0.000 abstract description 13
- 101710086987 X protein Proteins 0.000 abstract description 13
- 102000005600 Cathepsins Human genes 0.000 abstract description 6
- 108010084457 Cathepsins Proteins 0.000 abstract description 6
- 108010022172 Chitinases Proteins 0.000 abstract description 5
- 102000012286 Chitinases Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 102100038982 Exosome complex component RRP40 Human genes 0.000 description 12
- 230000000694 effects Effects 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 230000008034 disappearance Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 210000000663 muscle cell Anatomy 0.000 description 2
- 239000013608 rAAV vector Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 239000013607 AAV vector Substances 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 102100032106 Transmembrane emp24 domain-containing protein 3 Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14033—Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14051—Methods of production or purification of viral material
- C12N2710/14052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14144—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161512142P | 2011-07-27 | 2011-07-27 | |
| US201161512142P | 2011-07-27 | ||
| PCT/EP2012/064843 WO2013014294A2 (en) | 2011-07-27 | 2012-07-27 | Improved baculovirus expression systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2623780T3 true ES2623780T3 (es) | 2017-07-12 |
Family
ID=46640007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12745446.0T Active ES2623780T3 (es) | 2011-07-27 | 2012-07-27 | Sistemas de expresión de baculovirus mejorados |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10480010B2 (enExample) |
| EP (1) | EP2737071B1 (enExample) |
| JP (1) | JP6122430B2 (enExample) |
| CN (1) | CN103748229A (enExample) |
| BR (1) | BR112014001863A2 (enExample) |
| CA (1) | CA2842392C (enExample) |
| DK (1) | DK2737071T3 (enExample) |
| ES (1) | ES2623780T3 (enExample) |
| IN (1) | IN2014CN00688A (enExample) |
| WO (1) | WO2013014294A2 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10577627B2 (en) | 2014-06-09 | 2020-03-03 | Voyager Therapeutics, Inc. | Chimeric capsids |
| CN107106689A (zh) | 2014-11-05 | 2017-08-29 | 沃雅戈治疗公司 | 用于治疗帕金森病的aadc多核苷酸 |
| IL292999A (en) | 2014-11-14 | 2022-07-01 | Voyager Therapeutics Inc | Compositions and methods of treating amyotrophic lateral sclerosis (als) |
| CN112410338A (zh) | 2014-11-14 | 2021-02-26 | 沃雅戈治疗公司 | 调节性多核苷酸 |
| WO2016094783A1 (en) * | 2014-12-12 | 2016-06-16 | Voyager Therapeutics, Inc. | Compositions and methods for the production of scaav |
| WO2017189964A2 (en) | 2016-04-29 | 2017-11-02 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
| US11326182B2 (en) | 2016-04-29 | 2022-05-10 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
| SG11201809699XA (en) | 2016-05-18 | 2018-12-28 | Voyager Therapeutics Inc | Modulatory polynucleotides |
| KR20220108216A (ko) | 2016-05-18 | 2022-08-02 | 보이저 테라퓨틱스, 인크. | 헌팅톤 질환을 치료하기 위한 조성물 및 방법 |
| CN110650673B (zh) | 2016-08-30 | 2024-04-09 | 加利福尼亚大学董事会 | 用于生物医学靶向和递送的方法以及用于实践该方法的装置和系统 |
| SG11201909870SA (en) | 2017-05-05 | 2019-11-28 | Voyager Therapeutics Inc | Compositions and methods of treating amyotrophic lateral sclerosis (als) |
| JP2020518259A (ja) | 2017-05-05 | 2020-06-25 | ボイジャー セラピューティクス インコーポレイテッドＶｏｙａｇｅｒ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | ハンチントン病治療組成物および方法 |
| JOP20190269A1 (ar) | 2017-06-15 | 2019-11-20 | Voyager Therapeutics Inc | بولي نوكليوتيدات aadc لعلاج مرض باركنسون |
| JP7229989B2 (ja) | 2017-07-17 | 2023-02-28 | ボイジャー セラピューティクス インコーポレイテッド | 軌道アレイガイドシステム |
| TWI832036B (zh) | 2017-08-03 | 2024-02-11 | 美商航海家醫療公司 | 用於aav之遞送之組合物及方法 |
| MX2020003042A (es) | 2017-09-29 | 2020-11-18 | Voyager Therapeutics Inc | Rescate del fenotipo neurológico central y periférico de la ataxia de friedreich mediante administración intravenosa. |
| US11434502B2 (en) | 2017-10-16 | 2022-09-06 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis (ALS) |
| EP4454654A3 (en) | 2017-10-16 | 2025-02-19 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis (als) |
| EP3794126A1 (en) | 2018-05-15 | 2021-03-24 | Voyager Therapeutics, Inc. | Compositions and methods for the treatment of parkinson's disease |
| CN113383010B (zh) | 2018-09-28 | 2025-09-09 | 沃雅戈治疗公司 | 具有经工程化改造的启动子的共济蛋白表达构建体及其使用方法 |
| JP2022512621A (ja) * | 2018-10-05 | 2022-02-07 | ボイジャー セラピューティクス インコーポレイテッド | Ａａｖ産生タンパク質をコードする操作された核酸コンストラクト |
| EP3867389A1 (en) * | 2018-10-15 | 2021-08-25 | Voyager Therapeutics, Inc. | Expression vectors for large-scale production of raav in the baculovirus/sf9 system |
| CN109609552B (zh) * | 2018-12-28 | 2020-04-14 | 中国科学院武汉物理与数学研究所 | 重组腺相关病毒的制备方法、系统及重组杆粒 |
| TW202045529A (zh) | 2019-02-15 | 2020-12-16 | 美商聖加莫治療股份有限公司 | 用於生產重組aav之組合物及方法 |
| KR102272651B1 (ko) * | 2019-03-28 | 2021-07-02 | 충북대학교 산학협력단 | 외래단백질의 발현량과 발현시간이 증대된 배큘로바이러스 |
| JP7805169B2 (ja) * | 2019-06-26 | 2026-01-23 | ビロベク，インコーポレイテッド | バキュロウイルス発現系 |
| EP4229204A1 (en) | 2020-10-15 | 2023-08-23 | F. Hoffmann-La Roche AG | Nucleic acid constructs for simultaneous gene activation |
| CN114317608B (zh) * | 2020-12-28 | 2023-08-22 | 陕西杆粒生物科技有限公司 | 一种基因敲除型杆状病毒表达载体 |
| CN117716044A (zh) * | 2021-07-28 | 2024-03-15 | 生物马林药物股份有限公司 | 大规模腺相关病毒生产系统 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE78293T1 (de) | 1983-05-27 | 1992-08-15 | Texas A & M Univ Sys | Verfahren zur herstellung eines rekombinanten baculovirus-expressionsvektors. |
| US4745051A (en) | 1983-05-27 | 1988-05-17 | The Texas A&M University System | Method for producing a recombinant baculovirus expression vector |
| DK518384A (da) | 1984-01-31 | 1985-07-01 | Idaho Res Found | Vektor til fremstilling af et gen-produkt i insektceller, fremgangsmaade til dens fremstilling samt dens anvendelse |
| FR2664905B1 (fr) * | 1990-07-18 | 1994-08-12 | Agronomique Inst Nat Rech | Baculovirus modifie, son procede d'obtention, et vecteurs d'expression obtenus a partir dudit baculovirus. |
| CN1205741A (zh) * | 1995-12-22 | 1999-01-20 | 纳幕尔杜邦公司 | 重组杆状病毒的生产 |
-
2012
- 2012-07-27 IN IN688CHN2014 patent/IN2014CN00688A/en unknown
- 2012-07-27 BR BR112014001863A patent/BR112014001863A2/pt active Search and Examination
- 2012-07-27 EP EP12745446.0A patent/EP2737071B1/en not_active Not-in-force
- 2012-07-27 JP JP2014522116A patent/JP6122430B2/ja active Active
- 2012-07-27 CN CN201280037489.9A patent/CN103748229A/zh active Pending
- 2012-07-27 WO PCT/EP2012/064843 patent/WO2013014294A2/en not_active Ceased
- 2012-07-27 ES ES12745446.0T patent/ES2623780T3/es active Active
- 2012-07-27 CA CA2842392A patent/CA2842392C/en not_active Expired - Fee Related
- 2012-07-27 US US14/234,949 patent/US10480010B2/en not_active Expired - Fee Related
- 2012-07-27 DK DK12745446.0T patent/DK2737071T3/en active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2842392C (en) | 2020-09-15 |
| EP2737071A2 (en) | 2014-06-04 |
| US20140356904A1 (en) | 2014-12-04 |
| IN2014CN00688A (enExample) | 2015-04-03 |
| EP2737071B1 (en) | 2017-03-15 |
| JP2014522658A (ja) | 2014-09-08 |
| CA2842392A1 (en) | 2013-01-31 |
| US10480010B2 (en) | 2019-11-19 |
| BR112014001863A2 (pt) | 2017-02-21 |
| DK2737071T3 (en) | 2017-05-08 |
| WO2013014294A3 (en) | 2013-04-04 |
| JP6122430B2 (ja) | 2017-04-26 |
| WO2013014294A2 (en) | 2013-01-31 |
| CN103748229A (zh) | 2014-04-23 |
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