ES2578102T3 - Dispositivo y método para análisis de diagnóstico - Google Patents

Dispositivo y método para análisis de diagnóstico Download PDF

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Publication number
ES2578102T3
ES2578102T3 ES10732412.1T ES10732412T ES2578102T3 ES 2578102 T3 ES2578102 T3 ES 2578102T3 ES 10732412 T ES10732412 T ES 10732412T ES 2578102 T3 ES2578102 T3 ES 2578102T3
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Spain
Prior art keywords
biological samples
bacteria
containment
medium
wells
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ES10732412.1T
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English (en)
Inventor
Paolo Galiano
Gian Piero Spezzotti
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Alifax SRL
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Alifax Holding SpA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
    • G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Testing Or Measuring Of Semiconductors Or The Like (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

Abstract

Dispositivo integrado para análisis de diagnóstico usado para verificar la presencia de bacterias en al menos una muestra biológica mezclada con un medio de cultivo eugónico en forma líquida, para clasificar al menos el tipo de bacterias y para ensayar una serie de antibióticos, seleccionados de un grupo de antibióticos característico al menos para dicho tipo de bacterias identificado, identificando los que son eficaces para determinar la terapia antibiótica, caracterizado por que comprende, dentro de una estructura integrada (11): - primer medio de contención (12) provisto de elementos de contención (13) en los que se dispensan las muestras biológicas para analizar; - segundo medio de contención (14) que comprende recipientes (15) o microplacas (66) termostatizadas con pocillos (67) que contienen un medio de cultivo eugónico en forma líquida en el que se dispone una primera fracción de las muestras biológicas para analizar, y un primer recipiente (15a) y segundos recipientes (15b) o placas (66a, 66b) con un primer pocillo relativo (67a) y segundos pocillos (67b), en los que se dispone una fracción adicional de las muestras biológicas que dieron resultado positivo al análisis; - primer medio de examen (40, 49) capaz de verificar la presencia de bacterias en dichas muestras biológicas contenidas en dicho primer medio de contención (12) y dispensados en los recipientes (15) o microplacas (66) con pocillos (67) para detectar muestras biológicas positivas correspondientes y clasificar o identificar al menos el tipo de bacterias presente en dichas muestras biológicas positivas para seleccionar un grupo de antibióticos apropiados para estas; - segundo medio de examen (49, 40) capaz de verificar, en dichos primer recipiente (15a) o primer pocillo (67a) y en dichos segundos recipientes (15b) o segundos pocillos (67b), la respuesta sensible o resistente de cada muestra biológica positiva a una serie de antibióticos del grupo de antibióticos seleccionado por dicho primer medio de examen (40, 49); - tercer medio de contención (16) que comprende placas (116a, 116b) que contienen medios de cultivo sólido en los que se siembra una segunda fracción de la muestra biológica correspondiente a una muestra hallada positiva por dicho primer medio de examen (40, 49) para obtener el aislamiento y/o la identificación de dichas bacterias; - una unidad de movimiento y selección (20) controlada por una unidad de control (18) para realizar automáticamente al menos la toma de las muestras biológicas positivas y su dispensación en dicho medio de contención (14) y en dicho tercer medio de contención (16); en donde dicho primer medio de examen (40, 49) comprende un sistema de lectura provisto de medios capaces de detectar la obtención de un nivel dado de turbidez igual a 0,5 en la escala de McFarland de la suspensión bacteriana presente en dichos recipientes (15) o microplacas (66) con pocillos (67) obtenidos durante la etapa de crecimiento bacteriano exponencial.

Description

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Claims (1)

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ES10732412.1T 2009-02-25 2010-02-24 Dispositivo y método para análisis de diagnóstico Active ES2578102T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ITUD20090046 2009-02-25
ITUD2009A000046A IT1397120B1 (it) 2009-02-25 2009-02-25 Dispositivo integrato per analisi diagnostiche, e relativo procedimento
PCT/IB2010/000364 WO2010097683A2 (en) 2009-02-25 2010-02-24 Integrated device for diagnostic analyses, and relative method

Publications (1)

Publication Number Publication Date
ES2578102T3 true ES2578102T3 (es) 2016-07-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES10732412.1T Active ES2578102T3 (es) 2009-02-25 2010-02-24 Dispositivo y método para análisis de diagnóstico

Country Status (9)

Country Link
US (1) US8927258B2 (es)
EP (1) EP2401356B1 (es)
JP (1) JP5702738B2 (es)
KR (2) KR101914599B1 (es)
CN (1) CN102361968A (es)
CA (1) CA2753159C (es)
ES (1) ES2578102T3 (es)
IT (1) IT1397120B1 (es)
WO (1) WO2010097683A2 (es)

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EP3748010A1 (en) 2013-07-15 2020-12-09 President and Fellows of Harvard College Assays for antimicrobial activity and applications thereof
EP3071965A1 (en) 2013-11-21 2016-09-28 Avails Medical, Inc. Electrical biosensor for detecting a substance in a bodily fluid, and method and system for same
FR3015039B1 (fr) * 2013-12-12 2017-02-24 Stago Diagnostica Procede de determination de la position d'au moins un pion de cartographie
CN103923827B (zh) * 2014-04-25 2016-01-20 山东省食品药品检验研究院 全自动定量微生物制备仪
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US10254245B2 (en) 2016-01-25 2019-04-09 Avails Medical, Inc. Devices, systems and methods for detecting viable infectious agents in a fluid sample using an electrolyte-insulator-semiconductor sensor
CA3021760A1 (en) * 2016-04-25 2017-11-02 Aperture Bio, LLC Systems, devices and methods for sequential analysis of complex matrix samples for high confidence bacterial detection and drug susceptibility prediction using a flow cytometer
US10174356B2 (en) 2016-05-31 2019-01-08 Avails Medical, Inc. Devices, systems and methods to detect viable infectious agents in a fluid sample and susceptibility of infectious agents to anti-infectives
JP6829950B2 (ja) * 2016-05-31 2021-02-17 シスメックス株式会社 分析方法、分析装置および分析システム
IT201600069410A1 (it) * 2016-07-04 2018-01-04 Alifax Srl Apparato integrato per analisi diagnostiche
WO2018056584A1 (ko) 2016-09-21 2018-03-29 삼성전자 주식회사 피부 상태 측정 방법 및 이를 위한 전자 장치
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Also Published As

Publication number Publication date
EP2401356A2 (en) 2012-01-04
EP2401356B1 (en) 2016-04-20
WO2010097683A3 (en) 2010-11-04
WO2010097683A2 (en) 2010-09-02
KR20170080707A (ko) 2017-07-10
US20110306032A1 (en) 2011-12-15
CN102361968A (zh) 2012-02-22
KR101914599B1 (ko) 2018-11-02
ITUD20090046A1 (it) 2010-08-26
CA2753159A1 (en) 2010-09-02
IT1397120B1 (it) 2012-12-28
JP5702738B2 (ja) 2015-04-15
KR101753764B1 (ko) 2017-07-06
US8927258B2 (en) 2015-01-06
KR20110131228A (ko) 2011-12-06
JP2012518427A (ja) 2012-08-16
CA2753159C (en) 2020-03-24

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