ES2577293T3 - Utilización de un polisacárido excretado por la especie Vibrio diabolicus para ingeniería de tejidos conectivos no mineralizados - Google Patents
Utilización de un polisacárido excretado por la especie Vibrio diabolicus para ingeniería de tejidos conectivos no mineralizados Download PDFInfo
- Publication number
- ES2577293T3 ES2577293T3 ES06841875.5T ES06841875T ES2577293T3 ES 2577293 T3 ES2577293 T3 ES 2577293T3 ES 06841875 T ES06841875 T ES 06841875T ES 2577293 T3 ES2577293 T3 ES 2577293T3
- Authority
- ES
- Spain
- Prior art keywords
- connective tissues
- polysaccharide
- engineering non
- species vibrio
- mineralized connective
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 150000004676 glycans Chemical class 0.000 title abstract 3
- 229920001282 polysaccharide Polymers 0.000 title abstract 3
- 239000005017 polysaccharide Substances 0.000 title abstract 3
- 210000002808 connective tissue Anatomy 0.000 title description 2
- 241000501799 Vibrio diabolicus Species 0.000 title 1
- 102000008186 Collagen Human genes 0.000 abstract description 9
- 108010035532 Collagen Proteins 0.000 abstract description 9
- 229920001436 collagen Polymers 0.000 abstract description 9
- 239000011159 matrix material Substances 0.000 abstract description 3
- 230000003252 repetitive effect Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 229920002444 Exopolysaccharide Polymers 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 206010061592 cardiac fibrillation Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000002600 fibrillogenic effect Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
Landscapes
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Artificial Filaments (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Matriz de colágeno que comprende un polisacárido o una sal de dicho polisacárido de masa molar promedio en peso comprendida entre 500.000 y 2.000.000 g/mol, preferentemente comprendida entre 700.000 y 900.000 g/mol, caracterizada por 5 una secuencia osídica repetitiva lineal que incluye los siguientes 4 residuos osídicos: [(-3)-DGlcNacß(1-4)DGIcAß(1-4)DGIcAß(1-4)DGaINacα(1-)]
Description
La tinción con rojo sirio (tinción de Junquera) permite teñir de manera específica los colágenos, en la piel por ejemplo sus colágenos que aparecen como una estructura filamentosa suelta, teñida en rojo.
5 Las tinciones con rojo sirio de las secciones histológicas tras la observación con luz transmitida y luz polarizada muestran que la adición de HE800 durante la formación del entramado permite la formación de una matriz mucho más densa y en plazos mucho más breves que en los entramados de control.
10 Por ejemplo: la densidad de la matriz de colágeno del control tras 40 días de cultivo es equivalente a la observada en las matrices de colágeno formadas en presencia de HE800 en 11 días de cultivo. Este efecto sobre la densidad es mucho mayor en las dosis más bajas (10 %, 5 %).
15 La microscopía electrónica se realizó en tejidos conectivos equivalentes cultivados durante 11 días. Se observó un buen estado ultraestructural de las células, ya sea en el control o en los entramados formados en presencia de diferentes concentraciones de HE800.
20 No se ha podido observar fibra alguna de colágeno en los entramados de control, los entramados que comprenden el 20 % de HE800 permiten observar algunos elementos fibrilares recogidos en un gel que consiste en exopolisacáridos. Los entramados que comprenden el 10 % y el 5 % de los exopolisacáridos son muy diferentes, en efecto numerosas fibras de colágeno están presentes, se distribuyen por todo el entramado y presentan, en algunas, una estriación periódica. Estas fibras o fibrillas de colágeno se capturan en el gel formado por ΗE800.
25
Conclusión: HE800 acelera la fibrilación de colágeno y favorece la formación de una matriz extracelular.
10
Claims (1)
-
imagen1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0512413 | 2005-12-07 | ||
| FR0512413A FR2894146B1 (fr) | 2005-12-07 | 2005-12-07 | Utilisation d'un polysaccharide excrete par l'espece vibrio diabolicus a des fins d'ingenierie des tissus conjonctifs non mineralises |
| PCT/FR2006/002668 WO2007066009A1 (fr) | 2005-12-07 | 2006-12-06 | Utilisation d' un polysaccharide excrete par l'espece vibrio diabolicus a des fins d'ingenierie des tissus conjonctifs non mineralises |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2577293T3 true ES2577293T3 (es) | 2016-07-14 |
Family
ID=37026981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06841875.5T Active ES2577293T3 (es) | 2005-12-07 | 2006-12-06 | Utilización de un polisacárido excretado por la especie Vibrio diabolicus para ingeniería de tejidos conectivos no mineralizados |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20080317860A1 (es) |
| EP (1) | EP1960011B1 (es) |
| JP (1) | JP5154434B2 (es) |
| AU (1) | AU2006323841B2 (es) |
| ES (1) | ES2577293T3 (es) |
| FR (1) | FR2894146B1 (es) |
| WO (1) | WO2007066009A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103622764A (zh) * | 2012-08-23 | 2014-03-12 | 上海国睿生命科技有限公司 | 一种组织工程软骨的构建方法 |
| WO2014147255A1 (en) * | 2013-03-22 | 2014-09-25 | Lipotec S.A. | Exopolysaccharide for the treatment and/or care of the skin, mucous membranes and/or nails |
| JP7097433B2 (ja) | 2017-08-17 | 2022-07-07 | ジャスト－エヴォテック バイオロジックス、インコーポレイテッド | 宿主細胞ガレクチンおよび他の夾雑物からグリコシル化タンパク質を精製する方法 |
| US20230233598A1 (en) | 2020-07-02 | 2023-07-27 | Institut Francais De Recherche Pour L'exploitation De La Mer (Ifremer) | Marine Bacterial Exopolysaccharide Derivatives and Uses Thereof in the Treatment of Mucopolysaccharidoses |
| EP4112060A1 (en) | 2021-07-01 | 2023-01-04 | Institut Francais de Recherche pour l'Exploitation de la Mer (IFREMER) | Low-molecular-weight he800 exopolysaccharide derivatives with anti-cancer properties and uses thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4280954A (en) * | 1975-07-15 | 1981-07-28 | Massachusetts Institute Of Technology | Crosslinked collagen-mucopolysaccharide composite materials |
| US6179872B1 (en) * | 1998-03-17 | 2001-01-30 | Tissue Engineering | Biopolymer matt for use in tissue repair and reconstruction |
| DE19855890A1 (de) * | 1998-12-03 | 2000-06-08 | Nerlich Michael | Poröse Kompositmatrix, deren Herstellung und Verwendung |
| FR2811229B1 (fr) * | 2000-07-04 | 2003-08-01 | Ifremer | Utilisation d'un polysaccharide excrete par l'espece vibrio diabolicus en cicatrisation osseuse |
| JP4187917B2 (ja) * | 2000-09-08 | 2008-11-26 | 独立行政法人科学技術振興機構 | 組織再生マトリックス用グリコサミノグリカン−コラーゲン複合体の製造方法 |
| CA2442315A1 (en) * | 2001-04-02 | 2002-10-17 | Netech Inc. | Glycosaminoglycan/collagen complexes and use thereof |
| US20050142161A1 (en) * | 2003-12-30 | 2005-06-30 | Freeman Lynetta J. | Collagen matrix for soft tissue augmentation |
-
2005
- 2005-12-07 FR FR0512413A patent/FR2894146B1/fr active Active
-
2006
- 2006-12-06 ES ES06841875.5T patent/ES2577293T3/es active Active
- 2006-12-06 JP JP2008543868A patent/JP5154434B2/ja not_active Expired - Fee Related
- 2006-12-06 AU AU2006323841A patent/AU2006323841B2/en not_active Ceased
- 2006-12-06 EP EP06841875.5A patent/EP1960011B1/fr active Active
- 2006-12-06 US US12/096,596 patent/US20080317860A1/en not_active Abandoned
- 2006-12-06 WO PCT/FR2006/002668 patent/WO2007066009A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| US20080317860A1 (en) | 2008-12-25 |
| AU2006323841A1 (en) | 2007-06-14 |
| JP2009518091A (ja) | 2009-05-07 |
| AU2006323841B2 (en) | 2012-11-15 |
| FR2894146A1 (fr) | 2007-06-08 |
| JP5154434B2 (ja) | 2013-02-27 |
| EP1960011A1 (fr) | 2008-08-27 |
| EP1960011B1 (fr) | 2016-04-06 |
| WO2007066009A1 (fr) | 2007-06-14 |
| FR2894146B1 (fr) | 2008-06-06 |
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