ES2566501T3 - Detección del consumo de cannabis - Google Patents

Detección del consumo de cannabis Download PDF

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Publication number
ES2566501T3
ES2566501T3 ES09761850.8T ES09761850T ES2566501T3 ES 2566501 T3 ES2566501 T3 ES 2566501T3 ES 09761850 T ES09761850 T ES 09761850T ES 2566501 T3 ES2566501 T3 ES 2566501T3
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thc
fab
regions
detection
seq
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ES09761850.8T
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Kristiina Takkinen
Hans SÖDERLUND
Timo Pulli
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Valtion Teknillinen Tutkimuskeskus
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Valtion Teknillinen Tutkimuskeskus
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/948—Sedatives, e.g. cannabinoids, barbiturates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Anesthesiology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Geophysics And Detection Of Objects (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un fragmento de anticuerpo que reconoce específicamente un complejo inmunitario entre el tetrahidrocannabinol (THC) y el anti-THC, y que comprende las regiones determinantes de complementariedad (CDR) de una cadena ligera y de una cadena pesada de anticuerpo, en donde dichas regiones de la cadena ligera tienen las secuencias aminoacídicas de los aminoácidos 23 a 35, 51 a 57 y 93 a 100 de la SEQ ID n.º 3 y dichas regiones de la cadena pesada tienen las secuencias aminoacídicas de los aminoácidos 29 a 35, 50 a 60 y 99 a 109 de la SEQ ID n.º 4.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
inmovilizados (IMAC). El Fab 104 se purificó mediante Sepharose quelante con Ni2+ (Amersham Pharmacia Biotech). La pureza del Fab 104 se comprobó por SDS-PAGE.
Ejemplo 3
Un inmunoensayo homogéneo para el THC por transferencia de energía por resonancia de fluorescencia resuelta en 5 el tiempo (TR-FRET, por su nombre en inglés)
El fragmento Fab T3 anti-THC se marcó con europio mediante el kit LANCE Eu-W1024 ITC chelate (Wallac). El tampón del T3 marcado se cambió a Tris a 50 mM, pH 7,8, y NaCl al 0,9%. El fragmento Fab 104 contra el complejo inmunitario T3+THC se marcó con Alexa Fluor 647 con el kit de marcación de proteínas con Alexa Fluor (Molecular Probes, Inc.).
10 A los pocillos de microtitulación con 60 µl de saliva se les añadieron 1 µg del Fab T3 anti-THC marcado con Eu y 1,5 µg del Fab 104 contra el complejo inmunitario marcado con Alexa Fluor 647. A los pocillos se les añadieron muestras de 40 µl de saliva enriquecida con 0, 3,75, 7,5, 15, 30, 60 y 120 ng/ml de las siguientes drogas: D9-THC, 11-OH-D9-THC, 11-nor-D9-THC-COOH, heroína y anfetamina. Después de una mezcla breve, se midió la transferencia de energía por resonancia de fluorescencia resuelta en el tiempo (TR-FRET) con un fluorímetro Victor
15 V (Perkin Elmer Wallac). Los resultados se muestran en la figura 3.
La muestra de saliva enriquecida con THC da un valor fluorescente alto, mientras que los metabolitos del THC dan valores de fluorescencia significativamente más bajos, y la heroína y la morfina dan valores de fluorescencia cercanos a la fluorescencia de fondo detectada en el control (fragmentos T3 y 104 marcados a lo que no se ha añadido ninguna droga). El Fab 104 se fija al complejo inmunitario formado entre el T3 y el THC con una
20 especificidad y sensibilidad extremadamente altas. La prueba es capaz de detectar el THC muy por debajo del nivel de umbral (20 ng/ml en la saliva) de las pruebas rápidas que actualmente están disponibles en el mercado.
7

Claims (1)

  1. imagen1
ES09761850.8T 2008-06-12 2009-06-05 Detección del consumo de cannabis Active ES2566501T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20085579 2008-06-12
FI20085579A FI20085579A0 (fi) 2008-06-12 2008-06-12 Kannabiskäytön toteaminen
PCT/FI2009/050481 WO2009150295A1 (en) 2008-06-12 2009-06-05 Detection of cannabis use

Publications (1)

Publication Number Publication Date
ES2566501T3 true ES2566501T3 (es) 2016-04-13

Family

ID=39589343

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09761850.8T Active ES2566501T3 (es) 2008-06-12 2009-06-05 Detección del consumo de cannabis

Country Status (20)

Country Link
US (1) US8518653B2 (es)
EP (1) EP2297585B1 (es)
JP (1) JP5675597B2 (es)
CN (1) CN102057277B (es)
AU (1) AU2009256482B2 (es)
BR (1) BRPI0915086B8 (es)
CA (1) CA2724660C (es)
CY (1) CY1117631T1 (es)
DK (1) DK2297585T3 (es)
EA (1) EA019664B1 (es)
ES (1) ES2566501T3 (es)
FI (1) FI20085579A0 (es)
HR (1) HRP20160237T1 (es)
HU (1) HUE028677T2 (es)
MX (1) MX2010013692A (es)
NZ (1) NZ589300A (es)
PL (1) PL2297585T3 (es)
SI (1) SI2297585T1 (es)
WO (1) WO2009150295A1 (es)
ZA (1) ZA201008491B (es)

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JP2014511174A (ja) 2011-02-07 2014-05-15 ネオトープ バイオサイエンシーズ リミテッド Ａｐｏｅ免疫療法
JP6156144B2 (ja) * 2011-09-21 2017-07-05 富士レビオ株式会社 親和性複合体に対する抗体
US9970950B1 (en) 2014-03-09 2018-05-15 Hound Labs, Inc. Method and apparatus for detecting acute use of target substance(s)
WO2015142574A1 (en) 2014-03-21 2015-09-24 St & T International, Inc. Cannabis extraction method and compositions
US10308712B2 (en) 2014-03-27 2019-06-04 Bird Rock Bio, Inc. Antibodies that bind human cannabinoid 1 (CB1) receptor
US10319475B1 (en) 2014-06-13 2019-06-11 Enigami Systems, Inc. Method and apparatus for determining relationships between medications and symptoms
US9726684B1 (en) 2015-01-18 2017-08-08 Hound Labs, Inc. Compositions for target substance detection and measurement
US9585867B2 (en) 2015-08-06 2017-03-07 Charles Everett Ankner Cannabinod formulation for the sedation of a human or animal
EP3356833A4 (en) 2015-09-30 2019-04-10 Bird Rock Bio, Inc. ANTIBODIES FOR BINDING HUMAN CANNABINOID RECEPTOR 1 (CB1)
US9823259B1 (en) 2016-04-08 2017-11-21 Walter Nichols Detection device for cannabinoid use
US9933445B1 (en) 2016-05-16 2018-04-03 Hound Labs, Inc. System and method for target substance identification
US9921234B1 (en) 2016-06-17 2018-03-20 Hound Labs, Inc. Compositions and methods for detection of target constituent in exhaled breath
US11026596B1 (en) 2017-05-19 2021-06-08 Hound Labs, Inc. Detection and measurement of target substance in exhaled breath
US11187711B1 (en) 2017-09-11 2021-11-30 Hound Labs, Inc. Analyte detection from breath samples
US11860174B2 (en) 2017-12-15 2024-01-02 Bomi LLC Anti-cannabidiol antibody and uses thereof
US20200035118A1 (en) 2018-07-27 2020-01-30 Joseph Pandolfino Methods and products to facilitate smokers switching to a tobacco heating product or e-cigarettes
US10897925B2 (en) 2018-07-27 2021-01-26 Joseph Pandolfino Articles and formulations for smoking products and vaporizers
US11426097B1 (en) 2018-10-17 2022-08-30 Hound Labs, Inc. Rotary valve assemblies and methods of use for breath sample cartridge systems
US20200245898A1 (en) 2019-01-31 2020-08-06 Hound Labs, Inc. Single-use Microfluidic Cartridge for Detection of Target Chemical Presence in Human Breath
US11977086B2 (en) 2019-03-21 2024-05-07 Hound Labs, Inc. Biomarker detection from breath samples
US11933731B1 (en) 2020-05-13 2024-03-19 Hound Labs, Inc. Systems and methods using Surface-Enhanced Raman Spectroscopy for detecting tetrahydrocannabinol
US11806711B1 (en) 2021-01-12 2023-11-07 Hound Labs, Inc. Systems, devices, and methods for fluidic processing of biological or chemical samples using flexible fluidic circuits

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DE3433652A1 (de) * 1984-09-13 1986-03-20 Boehringer Mannheim Gmbh, 6800 Mannheim Immunchemisches messverfahren fuer haptene und proteine
WO1987007147A1 (en) * 1986-05-28 1987-12-03 Murex Corporation Method for producing monoclonal antibodies to an immunogenic antibody-antigen conjunction
US5223441A (en) 1986-10-09 1993-06-29 Syntex (U.S.A.) Inc. Receptors for immune complexes
JPH05504841A (ja) * 1990-09-14 1993-07-22 バイオサイト・ダイアグノスティックス・インコーポレイテッド リガンドレセプター及びリガンドのコンプレックスに対する抗体及びリガンド―レセプターアッセーでのそれらの用途
FI20022048A0 (fi) 2002-11-18 2002-11-18 Valtion Teknillinen Ei-kompetetiivinen immunomääritys pienille analyyteille
JP5586952B2 (ja) * 2006-06-06 2014-09-10 クルセル ホランド ベー ヴェー 腸球菌に対する殺活性を有するヒトの結合分子及びその使用方法

Also Published As

Publication number Publication date
AU2009256482A1 (en) 2009-12-17
JP5675597B2 (ja) 2015-02-25
CN102057277A (zh) 2011-05-11
US8518653B2 (en) 2013-08-27
EA201071435A1 (ru) 2011-08-30
CN102057277B (zh) 2014-12-24
EP2297585A1 (en) 2011-03-23
EA019664B1 (ru) 2014-05-30
HUE028677T2 (en) 2016-12-28
NZ589300A (en) 2011-11-25
PL2297585T3 (pl) 2016-09-30
DK2297585T3 (en) 2016-03-29
BRPI0915086B8 (pt) 2021-07-27
FI20085579A0 (fi) 2008-06-12
US20110086364A1 (en) 2011-04-14
SI2297585T1 (sl) 2016-06-30
AU2009256482B2 (en) 2014-11-13
JP2011524013A (ja) 2011-08-25
CY1117631T1 (el) 2017-04-26
ZA201008491B (en) 2011-08-31
CA2724660C (en) 2019-07-09
EP2297585B1 (en) 2016-03-02
CA2724660A1 (en) 2009-12-17
BRPI0915086A2 (pt) 2016-07-26
MX2010013692A (es) 2011-03-04
EP2297585A4 (en) 2012-05-02
BRPI0915086B1 (pt) 2020-05-12
WO2009150295A1 (en) 2009-12-17
HRP20160237T1 (hr) 2016-04-22

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