ES2552356T3 - Procedimiento para la caracterización de la actividad biológica de huevos de Trichuris - Google Patents
Procedimiento para la caracterización de la actividad biológica de huevos de Trichuris Download PDFInfo
- Publication number
- ES2552356T3 ES2552356T3 ES09769081.2T ES09769081T ES2552356T3 ES 2552356 T3 ES2552356 T3 ES 2552356T3 ES 09769081 T ES09769081 T ES 09769081T ES 2552356 T3 ES2552356 T3 ES 2552356T3
- Authority
- ES
- Spain
- Prior art keywords
- eggs
- determination
- embryonated
- trichuris
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000013601 eggs Nutrition 0.000 title abstract description 58
- 238000000034 method Methods 0.000 title abstract description 19
- 230000004071 biological effect Effects 0.000 title abstract description 11
- 241001489151 Trichuris Species 0.000 title abstract description 6
- 238000012512 characterization method Methods 0.000 title 1
- 230000004899 motility Effects 0.000 abstract description 22
- 230000012447 hatching Effects 0.000 abstract description 17
- 244000000013 helminth Species 0.000 abstract description 10
- 108010034145 Helminth Proteins Proteins 0.000 abstract description 9
- 238000010171 animal model Methods 0.000 abstract description 8
- 230000013020 embryo development Effects 0.000 abstract description 8
- 210000003736 gastrointestinal content Anatomy 0.000 abstract description 7
- 230000002503 metabolic effect Effects 0.000 abstract description 5
- 230000014509 gene expression Effects 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000011534 incubation Methods 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000003753 real-time PCR Methods 0.000 abstract description 3
- 102000012286 Chitinases Human genes 0.000 abstract 1
- 108010022172 Chitinases Proteins 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 230000001418 larval effect Effects 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000000825 pharmaceutical preparation Substances 0.000 abstract 1
- 229940127557 pharmaceutical product Drugs 0.000 abstract 1
- 125000003831 tetrazolyl group Chemical group 0.000 abstract 1
- 238000012795 verification Methods 0.000 abstract 1
- 241000960389 Trichuris suis Species 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 238000000338 in vitro Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 230000004913 activation Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 239000007850 fluorescent dye Substances 0.000 description 6
- 210000000936 intestine Anatomy 0.000 description 6
- 210000003278 egg shell Anatomy 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 230000033001 locomotion Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 210000001082 somatic cell Anatomy 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 241000361919 Metaphire sieboldi Species 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000007653 larval development Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43526—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08009344A EP2123774A1 (de) | 2008-05-21 | 2008-05-21 | Verfahren zur Charakterisierung der biologischen Aktivität von Helminth-Eiern |
| EP08009344 | 2008-05-21 | ||
| PCT/EP2009/056106 WO2009156232A1 (de) | 2008-05-21 | 2009-05-20 | Verfahren zur charakterisierung der biologischen aktivität von helminth-eiern, nämlich von trichuris eiern |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2552356T3 true ES2552356T3 (es) | 2015-11-27 |
Family
ID=39768691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09769081.2T Active ES2552356T3 (es) | 2008-05-21 | 2009-05-20 | Procedimiento para la caracterización de la actividad biológica de huevos de Trichuris |
Country Status (17)
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2492637C2 (ru) * | 2011-07-26 | 2013-09-20 | Закрытое акционерное общество "АЛЬФА-ТЭК" | Способ предынкубационной обработки яиц препаратом галосепт |
| US9224200B2 (en) | 2012-04-27 | 2015-12-29 | Parasite Technologies A/S | Computer vision based method for extracting features relating to the developmental stages of Trichuris spp. eggs |
| RU2609145C2 (ru) * | 2015-06-08 | 2017-01-30 | ФАНО России Федеральное государственное бюджетное научное учреждение Всероссийский научно-исследовательский институт фундаментальной и прикладной паразитологии животных и растений им. К.И. Скрябина (ФГБНУ "ВНИИП им. К.И. Скрябина") | Способ сбора максимального количества зрелых (=оплодотворённых) яиц (in vitro) от самок возбудителя паразитарного зооноза trichuris (=trichocephalus) suis |
| US9603875B1 (en) | 2016-01-07 | 2017-03-28 | NeuOva, LLC | Method of making a consumable product with purified embryonated Trichuris suis ova |
| JP6231709B1 (ja) * | 2016-05-31 | 2017-11-15 | シスメックス株式会社 | 蛍光画像分析装置および分析方法 |
| CN106689063B (zh) * | 2016-12-07 | 2020-03-17 | 中国科学院动物研究所 | 一种稻螟赤眼蜂的人工繁育生产方法 |
| WO2025259635A1 (en) * | 2024-06-10 | 2025-12-18 | Parasight System Inc. | Methods for analyzing fecal matter using fluorochromes that covalently bond to parasitic material |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU740776B2 (en) | 1997-12-31 | 2001-11-15 | University Of Iowa Research Foundation, The | Use of parasitic biological agents for prevention and control of autoimmune diseases |
| US7083911B2 (en) * | 2001-02-16 | 2006-08-01 | Promega Corporation | Method for detection of ATP |
| PL1968618T3 (pl) * | 2005-12-30 | 2017-10-31 | Parasite Tech A/S | Kompozycja zawierająca jaja pasożytów oraz sposoby izolowania i przechowywania jaj pasożytów |
| DE102006023906A1 (de) * | 2006-05-19 | 2007-11-22 | Ovamed Gmbh | Verfahren zum Nachweis der Viabilität |
-
2008
- 2008-05-21 EP EP08009344A patent/EP2123774A1/de not_active Withdrawn
-
2009
- 2009-05-20 WO PCT/EP2009/056106 patent/WO2009156232A1/de not_active Ceased
- 2009-05-20 AU AU2009262381A patent/AU2009262381B2/en not_active Ceased
- 2009-05-20 PT PT97690812T patent/PT2279264E/pt unknown
- 2009-05-20 CA CA2723402A patent/CA2723402C/en not_active Expired - Fee Related
- 2009-05-20 HU HUE09769081A patent/HUE026151T2/en unknown
- 2009-05-20 US US12/993,517 patent/US8993332B2/en not_active Expired - Fee Related
- 2009-05-20 RU RU2010152016/10A patent/RU2540145C2/ru not_active IP Right Cessation
- 2009-05-20 JP JP2011509973A patent/JP5753778B2/ja not_active Expired - Fee Related
- 2009-05-20 HR HRP20151173TT patent/HRP20151173T1/hr unknown
- 2009-05-20 UA UAA201015255A patent/UA103769C2/ru unknown
- 2009-05-20 DK DK09769081.2T patent/DK2279264T3/en active
- 2009-05-20 EP EP09769081.2A patent/EP2279264B1/de active Active
- 2009-05-20 CN CN200980118522.9A patent/CN102037141B/zh not_active Expired - Fee Related
- 2009-05-20 SI SI200931292T patent/SI2279264T1/sl unknown
- 2009-05-20 ES ES09769081.2T patent/ES2552356T3/es active Active
- 2009-05-20 PL PL09769081T patent/PL2279264T3/pl unknown
-
2015
- 2015-11-13 CY CY20151101026T patent/CY1116916T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HRP20151173T1 (hr) | 2015-12-04 |
| EP2123774A1 (de) | 2009-11-25 |
| JP2011523847A (ja) | 2011-08-25 |
| WO2009156232A9 (de) | 2010-02-18 |
| US20120060229A9 (en) | 2012-03-08 |
| CN102037141B (zh) | 2014-02-26 |
| CA2723402C (en) | 2018-06-19 |
| UA103769C2 (ru) | 2013-11-25 |
| US8993332B2 (en) | 2015-03-31 |
| AU2009262381A1 (en) | 2009-12-30 |
| HK1147113A1 (en) | 2011-07-29 |
| CY1116916T1 (el) | 2017-04-05 |
| DK2279264T3 (en) | 2015-11-23 |
| EP2279264B1 (de) | 2015-08-19 |
| PL2279264T3 (pl) | 2016-01-29 |
| RU2010152016A (ru) | 2012-06-27 |
| CA2723402A1 (en) | 2009-12-30 |
| EP2279264A1 (de) | 2011-02-02 |
| HUE026151T2 (en) | 2016-05-30 |
| PT2279264E (pt) | 2015-11-20 |
| RU2540145C2 (ru) | 2015-02-10 |
| SI2279264T1 (sl) | 2015-11-30 |
| WO2009156232A1 (de) | 2009-12-30 |
| CN102037141A (zh) | 2011-04-27 |
| US20110191869A1 (en) | 2011-08-04 |
| JP5753778B2 (ja) | 2015-07-22 |
| AU2009262381B2 (en) | 2015-03-26 |
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