ES2498823T3 - Variante de lipasa - Google Patents
Variante de lipasa Download PDFInfo
- Publication number
- ES2498823T3 ES2498823T3 ES10180769.1T ES10180769T ES2498823T3 ES 2498823 T3 ES2498823 T3 ES 2498823T3 ES 10180769 T ES10180769 T ES 10180769T ES 2498823 T3 ES2498823 T3 ES 2498823T3
- Authority
- ES
- Spain
- Prior art keywords
- wild type
- amino acid
- type lipase
- lipase
- negative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Lipasa que es un polipéptido con una secuencia de aminoácidos que: a) tiene al menos un 90 % de identidad con la lipasa tipo salvaje derivada de la cepa de Humicola lanuginosa DSM 4109; b) en comparación con dicha lipasa tipo salvaje, comprende una sustitución de T231R; y i) comprende un aminoácido negativo en la posición E210 de dicha lipasa tipo salvaje; ii) comprende un aminoácido cargado negativamente en la región correspondiente a las posiciones 90-101 de dicha lipasa de tipo salvaje; y iii) comprende un aminoácido neutro o negativo en una posición correspondiente a N94 de dicha lipasa tipo salvaje y/o tiene una carga eléctrica neta neutra o negativa en la región correspondiente a las posiciones 90-101 de dicha lipasa tipo salvaje
Description
comprender la sustitución G91A.
[0026] La lipasa puede opcionalmente comprender sustituciones de uno o más aminoácidos adicionales. Tales sustituciones pueden, por ejemplo, ser hechas según principios conocidos en la técnica, por ejemplo sustituciones 5 descritas en WO92/05249, WO94/25577, WO95/22615, WO97/04079 y WO97/07202.
[0027] Una variante de lipasa con buen rendimiento de primer lavado se puede obtener modificando la Lipolasa de la 10 siguiente manera. Las sustituciones entre paréntesis son opcionales.
- T231R+ N233R
- N94K+ D96L+ T231R+ N233R+ Q249R+ 270P+ 271G+ 272L
- D96L+ T231R+ N233R
- G91A+ E99K+ T231R+ N233R +Q249R
- (N33Q) +D96L +T231R +N233R +Q249R +270PGL
- R209P +T231R +N233R
- (N33Q) +E99N +N101S +T231R +N233R +Q249R +270 PGL
- K24C +(N33Q) +D96S +T231R +N233R +Q249R +270 PCL
- (N33Q) +G91A +E99K +T231R +N233R +Q249R +270 PGL
- E1A +(N33Q) +G91A +E99K +T231R +N233R +Q249R +270 PGL
- (N33Q) +G91A +E99K +G255R +T231R +N233R +Q249R +270 PGL
- (N33Q) +G91A +E99K +T231R +N233R +T244R +Q249R +270 PGL
- G91A +E99K +T231R +N233R +Q249R
- E87K +G91D +D96L +G225P +T231 R +N233R +Q249R +N251D
- G91A +E99K +T231R +N233R +Q249R +270AGVF
- G91A +E99K +T189G +T231R +N233R +Q249R
- D102G +T231R +N233R +Q249R
- T231R +N233R +Q249R +270AGVF
- R209P +T231 R +N233R
- N33Q +N94K +D96L +T231R +N233R +Q249R +270PGLPFKRV
- N33Q +N94K +D96L +T231R +N233R +Q249R
- N33Q +D96S +T231R +N233R +Q249R
- N33Q +D96S +V2281 + +T231R +N233R +Q249R
- E1A +N33Q +G91A +E99K +T231R +N233R +Q249R +270PGLPFKRV
- N33Q +S83T +E87K +G91A + E99K +T231R +N233R +Q249R +270PGLPFKRV
- N33Q +G91A + E99K +T231R +N233R +Q249R +270PGLPFKRV
- T231R +N233R +270CP
- T231R +N233R +270RE
- N33Q +E99N +N101S +T231R +N233R +Q249R +270PGLPFKRV
- D62A +S83T + G91A +E99K +T231R +N233R +Q249R
- E99N +N101S +T231R +N233R +Q249R
- R84W +G91A +E99K +T231 R +N233R +Q249R
- G91A +E99K +T231R +N233R +Q249R +270SPG
- G91A +E99K +T231R +N233R +Q249R +270VVVP
- G91A +E99K +T231R +N233R +Q249R +270LLASSGRGGHR
- G91A +E99K +T231R +N233R +Q249R +270VTT
- G91A +E99K +T231R +N233R +Q249R +270VLQ
- G91A +E99K +T231R +N233R +Q249R +270TST
- G91A +E99K +T231R +N233R +Q249R +270LRI
- V60G +D62E +G91A +E99K +T231R +N233R +Q249R
- G91A +D96W +E99K +T231R +N233R +G263Q +L264A +I265T +G266S +T267A +L269N +270AGGFS
15 [0028] La nomenclatura usada aquí para definir mutaciones es esencialmente como se describe en WO92/05249. Así, T231R indica una sustitución de T en la posición 231 con R. PGL o 270P+271 G+272L indica la adición peptídica PGL fijada al C-terminal (L269).
20 [0029] En esta especificación, los aminoácidos se clasifican como cargados negativamente, cargados positivamente o eléctricamente neutros según su carga eléctrica a pH 10, que es típico del detergente de la invención. Así, los aminoácidos negativos son E, D, C (cisteína) e Y, particularmente E y D. Los aminoácidos positivos son R, K y H,
4
Claims (1)
-
imagen1 imagen2
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK44199 | 1999-03-31 | ||
DKPA199900441 | 1999-03-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2498823T3 true ES2498823T3 (es) | 2014-09-25 |
Family
ID=8093585
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES03024829.8T Expired - Lifetime ES2445330T3 (es) | 1999-03-31 | 2000-03-30 | Variante de lipasa |
ES10180769.1T Expired - Lifetime ES2498823T3 (es) | 1999-03-31 | 2000-03-30 | Variante de lipasa |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES03024829.8T Expired - Lifetime ES2445330T3 (es) | 1999-03-31 | 2000-03-30 | Variante de lipasa |
Country Status (6)
Country | Link |
---|---|
EP (3) | EP1428874B1 (es) |
CN (1) | CN100455663C (es) |
BR (1) | BRPI0009396B1 (es) |
CA (1) | CA2366843A1 (es) |
DK (2) | DK2277999T3 (es) |
ES (2) | ES2445330T3 (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1851311A2 (en) * | 2005-02-10 | 2007-11-07 | Novozymes A/S | Enzymatic enantioselective ester or amide hydrolysis or synthesis |
US20090047266A1 (en) * | 2005-06-24 | 2009-02-19 | Novozymes A/S | Lipases for Pharmaceutical Use |
CN105296445B (zh) * | 2006-01-23 | 2022-05-10 | 诺维信公司 | 脂肪酶变体 |
CA3081308C (en) | 2006-12-21 | 2024-02-20 | Novozymes A/S | Lipase variants for pharmaceutical use |
AU2013200626B2 (en) * | 2006-12-21 | 2015-03-05 | Novozymes A/S | Lipase variants for pharmaceutical use |
US20090217464A1 (en) * | 2008-02-29 | 2009-09-03 | Philip Frank Souter | Detergent composition comprising lipase |
DK3382003T3 (da) * | 2011-12-29 | 2021-09-06 | Novozymes As | Detergentsammensætninger med lipasevarianter |
WO2013113622A1 (en) * | 2012-02-03 | 2013-08-08 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
CN104736700B (zh) * | 2012-10-12 | 2019-02-01 | 丹尼斯科美国公司 | 包含脂解酶变体的组合物和方法 |
CA2966292C (en) * | 2014-12-22 | 2022-10-04 | Novozymes A/S | Detergent compositions, lipase variants and polynucleotides encoding same |
CN107969136B (zh) * | 2015-07-06 | 2021-12-21 | 诺维信公司 | 脂肪酶变体以及编码它们的多核苷酸 |
US11214777B2 (en) * | 2017-05-12 | 2022-01-04 | Basf Se | Method for using lipase enzymes for cleaning |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3750450T2 (de) | 1986-08-29 | 1995-01-05 | Novo Industri As | Enzymhaltiger Reinigungsmittelzusatz. |
DE3854249T2 (de) | 1987-08-28 | 1996-02-29 | Novonordisk As | Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen. |
WO1992005249A1 (en) | 1990-09-13 | 1992-04-02 | Novo Nordisk A/S | Lipase variants |
US5869438A (en) | 1990-09-13 | 1999-02-09 | Novo Nordisk A/S | Lipase variants |
DK46693D0 (es) | 1993-04-23 | 1993-04-23 | Novo Nordisk As | |
JP3715328B2 (ja) * | 1992-09-21 | 2005-11-09 | 株式会社東芝 | データ処理装置 |
EP0746618B1 (en) | 1994-02-22 | 2002-08-21 | Novozymes A/S | A method of preparing a variant of a lipolytic enzyme |
BR9509729A (pt) * | 1994-11-18 | 1997-09-30 | Procter & Gamble | Composições detergentes contendo lipase e protease |
EP0839186B1 (en) | 1995-07-14 | 2004-11-10 | Novozymes A/S | A modified enzyme with lipolytic activity |
EP0851913B1 (en) | 1995-08-11 | 2004-05-19 | Novozymes A/S | Novel lipolytic enzymes |
EP0897423B1 (en) | 1996-04-25 | 2004-10-13 | Novozymes A/S | Alkaline lipolytic enzyme |
WO1997043375A1 (en) | 1996-05-15 | 1997-11-20 | The Procter & Gamble Company | Detergent compositions comprising specific lipolytic enzyme and a specific surfactant system |
AU3938697A (en) | 1996-08-27 | 1998-03-19 | Novo Nordisk A/S | Novel lipolytic enzymes |
AU3247699A (en) * | 1998-02-17 | 1999-09-06 | Novo Nordisk A/S | Lipase variant |
DK1131416T3 (da) * | 1998-11-27 | 2009-10-26 | Novozymes As | Lipolytiske enzymvarianter |
CA2467415C (en) * | 2002-01-16 | 2014-03-11 | Novozymes A/S | Lipolytic enzyme variants and method for their production |
-
2000
- 2000-03-30 CA CA002366843A patent/CA2366843A1/en not_active Abandoned
- 2000-03-30 DK DK10180769.1T patent/DK2277999T3/da active
- 2000-03-30 BR BRPI0009396-3A patent/BRPI0009396B1/pt active IP Right Grant
- 2000-03-30 EP EP03024829.8A patent/EP1428874B1/en not_active Expired - Lifetime
- 2000-03-30 ES ES03024829.8T patent/ES2445330T3/es not_active Expired - Lifetime
- 2000-03-30 EP EP10180782A patent/EP2278000A1/en not_active Withdrawn
- 2000-03-30 CN CNB008059500A patent/CN100455663C/zh not_active Expired - Lifetime
- 2000-03-30 EP EP10180769.1A patent/EP2277999B1/en not_active Expired - Lifetime
- 2000-03-30 DK DK03024829.8T patent/DK1428874T3/en active
- 2000-03-30 ES ES10180769.1T patent/ES2498823T3/es not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP1428874B1 (en) | 2013-12-18 |
ES2445330T3 (es) | 2014-03-03 |
EP1428874A3 (en) | 2004-07-14 |
DK2277999T3 (da) | 2014-09-22 |
EP1428874A2 (en) | 2004-06-16 |
EP2277999B1 (en) | 2014-07-02 |
EP2278000A1 (en) | 2011-01-26 |
BR0009396A (pt) | 2002-01-08 |
DK1428874T3 (en) | 2014-03-10 |
CN100455663C (zh) | 2009-01-28 |
CA2366843A1 (en) | 2000-10-12 |
BRPI0009396B1 (pt) | 2021-01-05 |
CN1409760A (zh) | 2003-04-09 |
EP2277999A1 (en) | 2011-01-26 |
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