ES2370947B1 - Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440 - Google Patents

Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440 Download PDF

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Publication number
ES2370947B1
ES2370947B1 ES200931258A ES200931258A ES2370947B1 ES 2370947 B1 ES2370947 B1 ES 2370947B1 ES 200931258 A ES200931258 A ES 200931258A ES 200931258 A ES200931258 A ES 200931258A ES 2370947 B1 ES2370947 B1 ES 2370947B1
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Prior art keywords
strain
pseudomonas putida
lytic
pha
application
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200931258A
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English (en)
Spanish (es)
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ES2370947A1 (es
Inventor
Virginia Martinez Lopez
Eduardo Diaz Fernandez
Pedro Garcia Gonzalez
Jose Luis Garcia Lopez
Estrella Duque Martin De Oliva
Juan Luis Ramos Martin
Maria Auxiliadora Prieto Jimenez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
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Consejo Superior de Investigaciones Cientificas CSIC
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Priority to ES200931258A priority Critical patent/ES2370947B1/es
Priority to PCT/ES2010/070858 priority patent/WO2011086211A1/fr
Publication of ES2370947A1 publication Critical patent/ES2370947A1/es
Application granted granted Critical
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Expired - Fee Related legal-status Critical Current
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462Lysozyme (3.2.1.17)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/62Carboxylic acid esters
    • C12P7/625Polyesters of hydroxy carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES200931258A 2009-12-23 2009-12-23 Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440 Expired - Fee Related ES2370947B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES200931258A ES2370947B1 (es) 2009-12-23 2009-12-23 Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440
PCT/ES2010/070858 WO2011086211A1 (fr) 2009-12-23 2010-12-21 Système d'autolyse cellulaire pour le traitement de la biomasse bactérienne dans la production de polyhydroxyalkanoates chez pseudomonas putida kt2440

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200931258A ES2370947B1 (es) 2009-12-23 2009-12-23 Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440

Publications (2)

Publication Number Publication Date
ES2370947A1 ES2370947A1 (es) 2011-12-26
ES2370947B1 true ES2370947B1 (es) 2012-11-02

Family

ID=43983233

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200931258A Expired - Fee Related ES2370947B1 (es) 2009-12-23 2009-12-23 Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440

Country Status (2)

Country Link
ES (1) ES2370947B1 (fr)
WO (1) WO2011086211A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2458465B1 (es) * 2012-10-01 2015-02-11 Consejo Superior De Investigaciones Cientificas (Csic) Procedimiento de fermentación para la producción de polihidroxialcanoatos y derivados que comprende la utilizacion de predadores bacterianos
WO2014096276A1 (fr) 2012-12-21 2014-06-26 Carbios Préparation de poly(hydroxyacides gras) à longue chaîne
EP3819380A1 (fr) 2019-11-05 2021-05-12 Consejo Superior De Investigaciones Científicas (CSIC) Souches de pseudomonas putida recombinant pour la production de polyhydroxyalcanoate

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2318070T3 (es) * 1998-10-28 2009-05-01 Genentech, Inc. Procedimiento para la recuperacion facilitada de proteinas heterologas a partir de celulas bacterianas.
WO2009155357A1 (fr) * 2008-06-17 2009-12-23 Arizona Board Of Regents Acides nucléiques, bactéries, et procédés pour dégrader la couche de peptidoglycane d'une paroi cellulaire

Also Published As

Publication number Publication date
ES2370947A1 (es) 2011-12-26
WO2011086211A1 (fr) 2011-07-21

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