ES2338856B8 - Conjunto de cebadores, sondas, procedimiento y kit para el genotipadodel polimorfismo genetico - 1131t/c del gen apo a5. - Google Patents
Conjunto de cebadores, sondas, procedimiento y kit para el genotipadodel polimorfismo genetico - 1131t/c del gen apo a5. Download PDFInfo
- Publication number
- ES2338856B8 ES2338856B8 ES201000004A ES201000004A ES2338856B8 ES 2338856 B8 ES2338856 B8 ES 2338856B8 ES 201000004 A ES201000004 A ES 201000004A ES 201000004 A ES201000004 A ES 201000004A ES 2338856 B8 ES2338856 B8 ES 2338856B8
- Authority
- ES
- Spain
- Prior art keywords
- genotyping
- primers
- apo
- procedure
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000523 sample Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- RDEIXVOBVLKYNT-VQBXQJRRSA-N (2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(1-aminoethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(aminomethyl)oxan-2-yl]o Chemical compound OS(O)(=O)=O.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@@H](CN)O2)N)[C@@H](N)C[C@H]1N.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@H](O2)C(C)N)N)[C@@H](N)C[C@H]1N.O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N RDEIXVOBVLKYNT-VQBXQJRRSA-N 0.000 title 1
- 238000003205 genotyping method Methods 0.000 abstract 6
- 206010071602 Genetic polymorphism Diseases 0.000 abstract 4
- 238000001514 detection method Methods 0.000 abstract 2
- 101150056589 A5 gene Proteins 0.000 abstract 1
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/775—Apolipopeptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Conjunto de cebadores, sondas, procedimiento y
kit para el genotipado del polimorfismo genético -1131T/C del gen
APO A5.
La presente invención se refiere a un conjunto
de cebadores, sonda, procedimiento y kit para genotipado de
polimorfismos genéticos, más concretamente para el genotipado del
polimorfismo genético -1131 T/C del gen APO A5 mediante la
amplificación mediante PCR de una muestra de ADN usando el conjunto
de cebadores reivindicado, y la detección mediante fluorescencia y
genotipado del polimorfismo genético usando el conjunto de sondas
reivindicado. Las principales ventajas de la presente invención son:
gran rapidez que permite el genotipado a gran escala ya que la
reacción de PCR y la detección de la señal fluorescente son
simultáneas, asignación de genotipos automatizada y obtenible de
forma inmediata al finalizar la reacción, gran sensibilidad que
permite genotipar muestras empleando concentraciones muy bajas de
ADN, y menor riesgo de contaminación al tratarse de unensayo
homogéneo.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201000004A ES2338856B8 (es) | 2009-12-29 | 2009-12-29 | Conjunto de cebadores, sondas, procedimiento y kit para el genotipadodel polimorfismo genetico - 1131t/c del gen apo a5. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201000004A ES2338856B8 (es) | 2009-12-29 | 2009-12-29 | Conjunto de cebadores, sondas, procedimiento y kit para el genotipadodel polimorfismo genetico - 1131t/c del gen apo a5. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ES2338856A1 ES2338856A1 (es) | 2010-05-12 |
| ES2338856B2 ES2338856B2 (es) | 2011-01-27 |
| ES2338856B8 true ES2338856B8 (es) | 2011-07-20 |
Family
ID=42121368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES201000004A Active ES2338856B8 (es) | 2009-12-29 | 2009-12-29 | Conjunto de cebadores, sondas, procedimiento y kit para el genotipadodel polimorfismo genetico - 1131t/c del gen apo a5. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2338856B8 (es) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1566449A1 (en) * | 2004-02-18 | 2005-08-24 | Max-Delbrück-Centrum Für Molekulare Medizin | Use of haplotypes and SNPs in lipid-relevant genes for the analyses and diagnosis of cardiovascular diseases |
| WO2008144940A1 (en) * | 2007-05-31 | 2008-12-04 | The University Of Western Ontario | Biomarker for hypertriglyceridemia |
-
2009
- 2009-12-29 ES ES201000004A patent/ES2338856B8/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2338856B2 (es) | 2011-01-27 |
| ES2338856A1 (es) | 2010-05-12 |
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