ES2180543T3 - Seleccion basada en reacciones para la expresion de, y la concentracion de, restos cataliticos. - Google Patents

Seleccion basada en reacciones para la expresion de, y la concentracion de, restos cataliticos.

Info

Publication number
ES2180543T3
ES2180543T3 ES93907001T ES93907001T ES2180543T3 ES 2180543 T3 ES2180543 T3 ES 2180543T3 ES 93907001 T ES93907001 T ES 93907001T ES 93907001 T ES93907001 T ES 93907001T ES 2180543 T3 ES2180543 T3 ES 2180543T3
Authority
ES
Spain
Prior art keywords
population
catalytic
expression
moiety
recombinant virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93907001T
Other languages
English (en)
Inventor
Mark T Martin
Rodger G Smith
Michael J Darsley
David M Simpson
Gary F Blackburn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IGEN International Inc
Original Assignee
IGEN International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IGEN International Inc filed Critical IGEN International Inc
Application granted granted Critical
Publication of ES2180543T3 publication Critical patent/ES2180543T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses

Abstract

SE PRESENTAN Y REIVINDICAN LOS METODO PARA SELECCIONAR UN VIRUS RECOMBINANTE, FAGO O CELULA QUE EXPRESAN UN ANTICUERPO CATALITICO O UNA PORCION CATALITICA DEL MISMO O PARA SELECCIONAR LA ACTIVIDAD CATALITICA MEDIANTE UNA UNIDAD. EN EL METODO SE LLEVA A CABO UNA SELECCION DE LA ACTIVIDAD CATALITICA BASADA EN UNA REACCION. EL METODO SE PUEDE UTILIZAR TAMBIEN PARA CONCENTRAR (AUMENTAR LA PROPORCION DE UNIDADES CATALITICAS RESPECTO A LAS NO CATALITICAS) UNA MUESTRA QUE CONTENGA UNA UNIDAD CATALITICA O VIRUS, FAGOS O CELULAS QUE EXPRESAN UNA UNIDAD CATALITICA. LA SELECCION O CONCENTRACION SE PUEDEN LLEVAR A CABO UTILIZANDO UN INHIBIDOR BASADO EN UN MECANISMO, UN MOVIMIENTO ACELERADO POR UNA CATALISIS, UN ENLACE SUPERFICIAL, CAMBIOS EN EL COMPONENTE ENTALPICO DE ENLACE COMO UNA FUNCION DE LA TEMPERATURA, O CAMBIOS EN EL ENLACE MEDIANTE UNA COMPETICION, O COMBINACIONES DE LOS MISMOS. LA INVENCION TAMBIEN SE REFIERE A UN METODO PARA LA PRODUCCION DE UN VIRUS RECOMBINANTE O LINEA CELULAR QUE EXPRESEUNA UNIDAD CATALITICA TAL COMO UN ANTICUERPO CATALITICO O UNA PORCION CATALITICA DEL MISMO; Y ESTE METODO PUEDE CONSISTIR EN INFECTAR UN HUESPED ADECUADO CON VIRUS QUE SE SELECCIONAN PARA LA EXPRESION. ADEMAS SE PRESENTAN Y REIVINDICAN TAMBIEN LOS VIRUS RECOMBINANTES Y LAS LINEAS CELULARES QUE EXPRESAN DE ESTE MODO UNA UNIDAD CATALITICA TAL COMO UN ANTICUERPO CATALITICO O UNA PORCION CATALITICA DEL MISMO.
ES93907001T 1992-02-24 1993-02-24 Seleccion basada en reacciones para la expresion de, y la concentracion de, restos cataliticos. Expired - Lifetime ES2180543T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US84164892A 1992-02-24 1992-02-24

Publications (1)

Publication Number Publication Date
ES2180543T3 true ES2180543T3 (es) 2003-02-16

Family

ID=25285377

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93907001T Expired - Lifetime ES2180543T3 (es) 1992-02-24 1993-02-24 Seleccion basada en reacciones para la expresion de, y la concentracion de, restos cataliticos.

Country Status (12)

Country Link
US (4) US5631137A (es)
EP (2) EP1203824A3 (es)
JP (1) JP4125365B2 (es)
KR (1) KR100233950B1 (es)
AT (1) ATE221578T1 (es)
AU (1) AU675783B2 (es)
CA (1) CA2125405A1 (es)
DE (1) DE69332168T2 (es)
ES (1) ES2180543T3 (es)
IL (1) IL104829A (es)
WO (1) WO1993017124A1 (es)
ZA (1) ZA931263B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030069173A1 (en) * 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
ES2283005T3 (es) * 1995-07-21 2007-10-16 University Of Nebraska Board Of Regents Composiciones y procedimientos para catalizar la hidrolisis de gp 120 de hiv.
DE69628026T2 (de) 1995-09-07 2004-04-08 Novozymes A/S Nachweis der aktivität von enzymen für detergenzien durch phagen.
EP0935612A4 (en) * 1996-03-26 2001-12-12 Amrad Operations Pty Ltd Precursors of catalytic antibodies
AUPO930697A0 (en) * 1997-09-19 1997-10-09 Walter And Eliza Hall Institute Of Medical Research, The Catalytic antibodies and a method of producing same
WO2000011212A1 (en) * 1998-08-19 2000-03-02 Novozymes A/S Enzyme activity screen with direct substrate reloading
EP1950305A1 (en) 2001-05-09 2008-07-30 Monsanto Technology, LLC Tyr a genes and uses thereof
EP1411761B1 (en) 2001-05-09 2010-01-20 Monsanto Technology LLC Metabolite transporters
FR2831836B1 (fr) * 2001-11-08 2004-04-23 Inst Nat Polytech Grenoble Procede de formation de sites catalytiques sur la surface d'un support
WO2003072830A1 (en) * 2002-02-22 2003-09-04 Purdue Research Foundation Magnetic nanomaterials and methods for detection of biological materials
EP1532274A4 (en) * 2002-06-28 2006-09-27 Purdue Research Foundation MAGNETIC NANOMATERIALS AND METHODS FOR DETECTING BIOLOGICAL MATERIALS

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3745285A (en) * 1983-11-29 1985-06-13 Igen, Inc. Method of catalyzing chemical reactions
BE1006312A3 (fr) * 1991-11-29 1994-07-19 Univ Catholique Louvain Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique.

Also Published As

Publication number Publication date
US5631137A (en) 1997-05-20
IL104829A0 (en) 1993-06-10
AU3776193A (en) 1993-09-13
ATE221578T1 (de) 2002-08-15
CA2125405A1 (en) 1993-09-02
KR950700424A (ko) 1995-01-16
DE69332168D1 (de) 2002-09-05
JPH07504090A (ja) 1995-05-11
US6177270B1 (en) 2001-01-23
KR100233950B1 (ko) 1999-12-15
JP4125365B2 (ja) 2008-07-30
AU675783B2 (en) 1997-02-20
IL104829A (en) 1999-04-11
EP0631633A4 (en) 1998-02-25
EP0631633B1 (en) 2002-07-31
EP1203824A2 (en) 2002-05-08
ZA931263B (en) 1993-11-02
EP0631633A1 (en) 1995-01-04
US5891648A (en) 1999-04-06
WO1993017124A1 (en) 1993-09-02
EP1203824A3 (en) 2005-02-23
DE69332168T2 (de) 2003-03-20
US6121007A (en) 2000-09-19

Similar Documents

Publication Publication Date Title
AU5984790A (en) Method and apparatus for heterogeneous chemiluminescence assay
AU3882689A (en) Carrier matrix with dissolvably impregnated reagent
ES2180543T3 (es) Seleccion basada en reacciones para la expresion de, y la concentracion de, restos cataliticos.
SE8702511L (sv) Biospecifikt multianalytanalysfoerfarande
ATE257943T1 (de) Elektrochemische und photochemische elektroden und deren verwendung
ATE10208T1 (de) Stabilisierte, waessrige loesung von co-enzymen zur verwendung in klinischen analysen; verfahren zur stabilisierung eines labilen co-enzyms in einer waessrigen loesung fuer die klinische analyse und regenzsatz fuer diese klinische analyse.
DK0726322T4 (da) Assay for homocystein
ES8802258A1 (es) Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos
DE59203610D1 (de) Reaktor zur Durchführung biologischer Reaktionen mittels Biokatalysatoren.
CA2137655A1 (en) Sensor for optical assay
ATE103711T1 (de) Immuntestsatz und -verfahren fuer ganze zellen.
BR8603245A (pt) Processo de preparacao de esteres metacrilicos por transesterificacao
DE69310667T2 (de) Immunoassayverfahren unter Anwendung photothermischer Strahlablenkungsspektroskopie
AU2462388A (en) Method for self-performing enzyme kinetics
ATE76082T1 (de) Reinigungsmethode fuer antikoerper.
DE3863398D1 (de) Thermokatalytisches umwandlungsverfahren einer schweren kohlenwasserstoffbeschichtung.
EP0311388A3 (en) A rapid method for identifying a specific nucleic acid sequence
EP0184701A3 (en) A method for determining a ligand
JPS6422759A (en) Accommodating method with supporting clip
AU5287486A (en) Teststrip for multiple analytes using immunoassay or nucleic acid hybridization
ES2010940A6 (es) Procedimiento de preparacion de *-quimotripsina inmovilizada sobre tierra de diatomeas.
MY102245A (en) Purification of antibodies