ES2179818T3 - Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos. - Google Patents

Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos.

Info

Publication number
ES2179818T3
ES2179818T3 ES91920565T ES91920565T ES2179818T3 ES 2179818 T3 ES2179818 T3 ES 2179818T3 ES 91920565 T ES91920565 T ES 91920565T ES 91920565 T ES91920565 T ES 91920565T ES 2179818 T3 ES2179818 T3 ES 2179818T3
Authority
ES
Spain
Prior art keywords
antibiotic
dna
modified
libraries
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91920565T
Other languages
English (en)
Inventor
David Botstein
Timothy Palzkill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ES2179818T3 publication Critical patent/ES2179818T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A METODOS Y ENLACES MUTAGENOS PARA MODIFICAR DNA, A METODOS PARA REALIZAR BIBLIOTECAS QUE CONTIENEN UNA GRAN DIVERSIDAD DE DNA MODIFICADOS Y A METODOS PARA UTILIZAR TALES BIBLIOTECAS PARA PROTEGER PROTEINAS MODIFICADAS CODIFICADAS POR ESTOS DNA. EL DNA ELEGIDO PARA LA MODIFICACION NORMALMENTE CODIFICA UN POLIPEPTIDO TAL COMO UNA ENZIMA. LAS BIBLIOTECAS SE UTILIZAN PARA DETERMINAR LOS EFECTOS DE ESTA MODIFICACION O DE LA INTERACCION ENTRE LOS POLIPEPTIDOS MODIFICADOS Y UN OBJETIVO. UNA VARIANTE PREFERIBLE DE LA INVENCION SE REFIERE A METODOS PARA REALIZAR Y UTILIZAR BIBLIOTECAS DE DNA QUE CODIFICAN HIDROLASAS ANTIBIOTICAS MODIFICADAS PARA PROTEGER ANTIBIOTICOS CONTRA UNA O MAS DE LAS HIDROLASAS ANTIBIOTICAS MODIFICADAS QUE SON PRODUCIDOS POR TALES BIBLIOTECAS. LA SUSCEPTIBILIDAD O LA FALTA DE ELLA A LA NEUTRALIZACION DE UN ANTIBIOTICO INDICA SI HIDROLASAS ANTIBIOTICAS TIPO SILVESTRE TIENDEN A MUTAR PARA CONCEDER RESISTENCIA AL ANTIBIOTICO.
ES91920565T 1990-10-22 1991-10-21 Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos. Expired - Lifetime ES2179818T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US60215890A 1990-10-22 1990-10-22

Publications (1)

Publication Number Publication Date
ES2179818T3 true ES2179818T3 (es) 2003-02-01

Family

ID=24410215

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91920565T Expired - Lifetime ES2179818T3 (es) 1990-10-22 1991-10-21 Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos.

Country Status (8)

Country Link
US (1) US5677153A (es)
EP (1) EP0555357B1 (es)
AT (1) ATE220113T1 (es)
CA (1) CA2094495C (es)
DE (1) DE69133054T2 (es)
DK (1) DK0555357T3 (es)
ES (1) ES2179818T3 (es)
WO (1) WO1992007090A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406848B1 (en) 1997-05-23 2002-06-18 Lynx Therapeutics, Inc. Planar arrays of microparticle-bound polynucleotides
US6500660B1 (en) 1996-11-27 2002-12-31 Université Catholique de Louvain Chimeric target molecules having a regulatable activity
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
KR100391884B1 (en) * 2002-07-04 2003-09-06 Amicogen Co Ltd Random codon based mutagenesis using transposon
US20040191873A1 (en) * 2003-03-27 2004-09-30 Won-Bin Young Method for high throughput elucidation of transcriptional profiles and genome annotation
US7323303B2 (en) * 2003-03-31 2008-01-29 Hong Kong Polytechnic University Modified β-lactamases and uses thereof
WO2005118877A2 (en) 2004-06-02 2005-12-15 Vicus Bioscience, Llc Producing, cataloging and classifying sequence tags
WO2006069099A2 (en) * 2004-12-21 2006-06-29 Genecopoeia, Inc. Method and compositions for rapidly modifying clones
CN101805747B (zh) * 2010-04-06 2012-05-09 东华大学 一种重复模块基因拼接方法
US20150337290A1 (en) * 2012-09-07 2015-11-26 Alexander Gubin Methods and products for mutating nucleotide sequences

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
US4643969A (en) * 1983-07-25 1987-02-17 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4935357A (en) * 1986-02-05 1990-06-19 New England Biolabs, Inc. Universal restriction endonuclease
GB8818150D0 (en) * 1988-07-29 1988-09-01 Crc Technology Gene modification
ATE152174T1 (de) * 1989-09-20 1997-05-15 Abbott Lab Verfahren zur herstellung von fusionsproteinen

Also Published As

Publication number Publication date
ATE220113T1 (de) 2002-07-15
CA2094495A1 (en) 1992-04-23
DE69133054T2 (de) 2002-11-14
US5677153A (en) 1997-10-14
DE69133054D1 (de) 2002-08-08
WO1992007090A1 (en) 1992-04-30
DK0555357T3 (da) 2002-10-28
EP0555357A4 (en) 1996-10-09
EP0555357A1 (en) 1993-08-18
EP0555357B1 (en) 2002-07-03
CA2094495C (en) 2007-04-03

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